Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo.
Porous calcium phosphate ceramics are used in orthopedic and craniofacial applications to treat bone loss, or in dental applications to replace missing teeth. The implantation of these materials, however, does not induce stem cell differentiation, so suitable additional materials such as porous calc...
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doaj-dfbdfcbf3af543ed934b28d2847d6a982020-11-24T21:45:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8427210.1371/journal.pone.0084272Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo.Hye-Rim LeeHan-Jun KimJi-Seung KoYong-Suk ChoiMyun-Whan AhnSukyoung KimSun Hee DoPorous calcium phosphate ceramics are used in orthopedic and craniofacial applications to treat bone loss, or in dental applications to replace missing teeth. The implantation of these materials, however, does not induce stem cell differentiation, so suitable additional materials such as porous calcium phosphate discs are needed to influence physicochemical responses or structural changes. Rabbit adipose-derived stem cells (ADSC) and mouse osteoblastic cells (MC3T3-E1) were evaluated in vitro by the MTT assay, semi-quantitative RT-PCR, and immunoblotting using cells cultured in medium supplemented with extracts from bioceramics, including calcium metaphosphate (CMP), hydroxyapatite (HA) and collagen-grafted HA (HA-col). In vivo evaluation of the bone forming capacity of these bioceramics in rat models using femur defects and intramuscular implants for 12 weeks was performed. Histological analysis showed that newly formed stromal-rich tissues were observed in all the implanted regions and that the implants showed positive immunoreaction against type I collagen and alkaline phosphatase (ALP). The intramuscular implant region, in particular, showed strong positive immunoreactivity for both type I collagen and ALP, which was further confirmed by mRNA expression and immunoblotting results, indicating that each bioceramic material enhanced osteogenesis stimulation. These results support our hypothesis that smart bioceramics can induce osteoconduction and osteoinduction in vivo, although mature bone formation, including lacunae, osteocytes, and mineralization, was not prominent until 12 weeks after implantation.http://europepmc.org/articles/PMC3877265?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hye-Rim Lee Han-Jun Kim Ji-Seung Ko Yong-Suk Choi Myun-Whan Ahn Sukyoung Kim Sun Hee Do |
spellingShingle |
Hye-Rim Lee Han-Jun Kim Ji-Seung Ko Yong-Suk Choi Myun-Whan Ahn Sukyoung Kim Sun Hee Do Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. PLoS ONE |
author_facet |
Hye-Rim Lee Han-Jun Kim Ji-Seung Ko Yong-Suk Choi Myun-Whan Ahn Sukyoung Kim Sun Hee Do |
author_sort |
Hye-Rim Lee |
title |
Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. |
title_short |
Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. |
title_full |
Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. |
title_fullStr |
Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. |
title_full_unstemmed |
Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. |
title_sort |
comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Porous calcium phosphate ceramics are used in orthopedic and craniofacial applications to treat bone loss, or in dental applications to replace missing teeth. The implantation of these materials, however, does not induce stem cell differentiation, so suitable additional materials such as porous calcium phosphate discs are needed to influence physicochemical responses or structural changes. Rabbit adipose-derived stem cells (ADSC) and mouse osteoblastic cells (MC3T3-E1) were evaluated in vitro by the MTT assay, semi-quantitative RT-PCR, and immunoblotting using cells cultured in medium supplemented with extracts from bioceramics, including calcium metaphosphate (CMP), hydroxyapatite (HA) and collagen-grafted HA (HA-col). In vivo evaluation of the bone forming capacity of these bioceramics in rat models using femur defects and intramuscular implants for 12 weeks was performed. Histological analysis showed that newly formed stromal-rich tissues were observed in all the implanted regions and that the implants showed positive immunoreaction against type I collagen and alkaline phosphatase (ALP). The intramuscular implant region, in particular, showed strong positive immunoreactivity for both type I collagen and ALP, which was further confirmed by mRNA expression and immunoblotting results, indicating that each bioceramic material enhanced osteogenesis stimulation. These results support our hypothesis that smart bioceramics can induce osteoconduction and osteoinduction in vivo, although mature bone formation, including lacunae, osteocytes, and mineralization, was not prominent until 12 weeks after implantation. |
url |
http://europepmc.org/articles/PMC3877265?pdf=render |
work_keys_str_mv |
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