Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop
The forage crop Guar (Cyamopsis tetragonoloba (L.) Taub.) has the ability to endure heat, drought, and mild salinity. A complete image on its genic architecture will promote our understanding about gene expression networks and different tolerance mechanisms at the molecular level. Therefore, whole m...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2019-01-01
|
Series: | International Journal of Genomics |
Online Access: | http://dx.doi.org/10.1155/2019/7295859 |
id |
doaj-dfdbc08c5de14d85a193c56d460bc41b |
---|---|
record_format |
Article |
spelling |
doaj-dfdbc08c5de14d85a193c56d460bc41b2020-11-25T02:08:38ZengHindawi LimitedInternational Journal of Genomics2314-436X2314-43782019-01-01201910.1155/2019/72958597295859Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage CropFahad Al-Qurainy0Aref Alshameri1Abdel-Rhman Gaafar2Salim Khan3Mohammad Nadeem4Abdulhafed Abdullah Alameri5Mohamed Tarroum6Muhammad Ashraf7Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaThe forage crop Guar (Cyamopsis tetragonoloba (L.) Taub.) has the ability to endure heat, drought, and mild salinity. A complete image on its genic architecture will promote our understanding about gene expression networks and different tolerance mechanisms at the molecular level. Therefore, whole mRNA sequence approach on the Guar plant was conducted to provide a snapshot of the mRNA information in the cell under salinity, heat, and drought stresses to be integrated with previous transcriptomic studies. RNA-Seq technology was employed to perform a 2×100 paired-end sequencing using an Illumina HiSeq 2500 platform for the transcriptome of leaves of C. tetragonoloba under normal, heat, drought, and salinity conditions. Trinity was used to achieve a de novo assembly followed by gene annotation, functional classification, metabolic pathway analysis, and identification of SSR markers. A total of 218.2 million paired-end raw reads (~44 Gbp) were generated. Of those, 193.5M paired-end reads of high quality were used to reconstruct a total of 161,058 transcripts (~266 Mbp) with N50 of 2552 bp and 61,508 putative genes. There were 6463 proteins having >90% full-length coverage against the Swiss-Prot database and 94% complete orthologs against Embryophyta. Approximately, 62.87% of transcripts were blasted, 50.46% mapped, and 43.50% annotated. A total of 4715 InterProScan families, 3441 domains, 74 repeats, and 490 sites were detected. Biological processes, molecular functions, and cellular components comprised 64.12%, 25.42%, and 10.4%, respectively. The transcriptome was associated with 985 enzymes and 156 KEGG pathways. A total of 27,066 SSRs were gained with an average frequency of one SSR/9.825 kb in the assembled transcripts. This resulting data will be helpful for the advanced analysis of Guar to multi-stress tolerance.http://dx.doi.org/10.1155/2019/7295859 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fahad Al-Qurainy Aref Alshameri Abdel-Rhman Gaafar Salim Khan Mohammad Nadeem Abdulhafed Abdullah Alameri Mohamed Tarroum Muhammad Ashraf |
spellingShingle |
Fahad Al-Qurainy Aref Alshameri Abdel-Rhman Gaafar Salim Khan Mohammad Nadeem Abdulhafed Abdullah Alameri Mohamed Tarroum Muhammad Ashraf Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop International Journal of Genomics |
author_facet |
Fahad Al-Qurainy Aref Alshameri Abdel-Rhman Gaafar Salim Khan Mohammad Nadeem Abdulhafed Abdullah Alameri Mohamed Tarroum Muhammad Ashraf |
author_sort |
Fahad Al-Qurainy |
title |
Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop |
title_short |
Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop |
title_full |
Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop |
title_fullStr |
Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop |
title_full_unstemmed |
Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop |
title_sort |
comprehensive stress-based de novo transcriptome assembly and annotation of guar (cyamopsis tetragonoloba (l.) taub.): an important industrial and forage crop |
publisher |
Hindawi Limited |
series |
International Journal of Genomics |
issn |
2314-436X 2314-4378 |
publishDate |
2019-01-01 |
description |
The forage crop Guar (Cyamopsis tetragonoloba (L.) Taub.) has the ability to endure heat, drought, and mild salinity. A complete image on its genic architecture will promote our understanding about gene expression networks and different tolerance mechanisms at the molecular level. Therefore, whole mRNA sequence approach on the Guar plant was conducted to provide a snapshot of the mRNA information in the cell under salinity, heat, and drought stresses to be integrated with previous transcriptomic studies. RNA-Seq technology was employed to perform a 2×100 paired-end sequencing using an Illumina HiSeq 2500 platform for the transcriptome of leaves of C. tetragonoloba under normal, heat, drought, and salinity conditions. Trinity was used to achieve a de novo assembly followed by gene annotation, functional classification, metabolic pathway analysis, and identification of SSR markers. A total of 218.2 million paired-end raw reads (~44 Gbp) were generated. Of those, 193.5M paired-end reads of high quality were used to reconstruct a total of 161,058 transcripts (~266 Mbp) with N50 of 2552 bp and 61,508 putative genes. There were 6463 proteins having >90% full-length coverage against the Swiss-Prot database and 94% complete orthologs against Embryophyta. Approximately, 62.87% of transcripts were blasted, 50.46% mapped, and 43.50% annotated. A total of 4715 InterProScan families, 3441 domains, 74 repeats, and 490 sites were detected. Biological processes, molecular functions, and cellular components comprised 64.12%, 25.42%, and 10.4%, respectively. The transcriptome was associated with 985 enzymes and 156 KEGG pathways. A total of 27,066 SSRs were gained with an average frequency of one SSR/9.825 kb in the assembled transcripts. This resulting data will be helpful for the advanced analysis of Guar to multi-stress tolerance. |
url |
http://dx.doi.org/10.1155/2019/7295859 |
work_keys_str_mv |
AT fahadalqurainy comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT arefalshameri comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT abdelrhmangaafar comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT salimkhan comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT mohammadnadeem comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT abdulhafedabdullahalameri comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT mohamedtarroum comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop AT muhammadashraf comprehensivestressbaseddenovotranscriptomeassemblyandannotationofguarcyamopsistetragonolobaltaubanimportantindustrialandforagecrop |
_version_ |
1724926485060386816 |