Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach
Brain inflammation is one of the hallmarks of Alzheimer disease (AD) and a current trend is that inflammatory mediators, particularly cytokines and chemokines, may represent valuable biomarkers for early screening and diagnosis of the disease. Various studies have reported differences in serum level...
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doaj-dfe3867be84242d68cbace0b18becdbb2020-11-24T22:01:08ZengFrontiers Media S.A.Frontiers in Neurology1664-22952015-08-01610.3389/fneur.2015.00181151621Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approachConstance eDELABY0Audrey eGABELLE1Audrey eGABELLE2David eBLUM3Susanna eSCHRAEN MASCHKE4Amandine eMOULINIER5Justine eBOULANGIEN6Dany eSEVERAC7Luc eBUEE8Thierry eREME9Sylvain eLEHMANN10Université Montpellier IUniversité Montpellier ICentre Mémoire Ressource Recherche Languedoc RoussillonINSERM U837INSERM U837Université Montpellier IUniversité Montpellier IInstitut de Génomique FonctionnelleINSERM U837CHU Montpellier, France.Université Montpellier IBrain inflammation is one of the hallmarks of Alzheimer disease (AD) and a current trend is that inflammatory mediators, particularly cytokines and chemokines, may represent valuable biomarkers for early screening and diagnosis of the disease. Various studies have reported differences in serum level of cytokines, chemokines, and growth factors in patients with MCI (Mild Cognitive Impairment) or AD. However, data were often inconsistent and the exact function of inflammation in neurodegeneration is still a matter of debate.In the present work, we measured the expression of 120 biomarkers (corresponding to cytokines, chemokines, growth factors, and related signalling proteins) in the serum of 49 patients with the following diagnosis distribution: 15 controls, 14 AD and 20 MCI. In addition, we performed the same analysis in the cerebrospinal fluid (CSF) of 20 of these patients (10 AD and 10 controls). Among the biomarkers tested, none showed significant changes in the serum, but 13 were significantly modified in the CSF of AD patients. Interestingly, all of these biomarkers were implicated in neurogenesis or neural stem cells migration and differentiation. In a second part of the study, 10 of these putative biomarkers (plus 4 additional) were quantified using quantitative multiplex ELISA methods in the CSF and the serum of an enlarged cohort composed of 31 AD and 24 control patients.Our results confirm the potential diagnosis interest of previously published blood biomarkers, and proposes new ones (such as IL-8 and TNFR-I). Further studies will be needed to validate these biomarkers which could be used alone, combined, or in association with the classical amyloid and tau biomarkers.http://journal.frontiersin.org/Journal/10.3389/fneur.2015.00181/fullAlzheimer DiseaseCerebrospinal FluidInflammationSerumbiomarkers |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Constance eDELABY Audrey eGABELLE Audrey eGABELLE David eBLUM Susanna eSCHRAEN MASCHKE Amandine eMOULINIER Justine eBOULANGIEN Dany eSEVERAC Luc eBUEE Thierry eREME Sylvain eLEHMANN |
spellingShingle |
Constance eDELABY Audrey eGABELLE Audrey eGABELLE David eBLUM Susanna eSCHRAEN MASCHKE Amandine eMOULINIER Justine eBOULANGIEN Dany eSEVERAC Luc eBUEE Thierry eREME Sylvain eLEHMANN Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach Frontiers in Neurology Alzheimer Disease Cerebrospinal Fluid Inflammation Serum biomarkers |
author_facet |
Constance eDELABY Audrey eGABELLE Audrey eGABELLE David eBLUM Susanna eSCHRAEN MASCHKE Amandine eMOULINIER Justine eBOULANGIEN Dany eSEVERAC Luc eBUEE Thierry eREME Sylvain eLEHMANN |
author_sort |
Constance eDELABY |
title |
Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach |
title_short |
Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach |
title_full |
Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach |
title_fullStr |
Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach |
title_full_unstemmed |
Central nervous system and peripheral inflammatory processes in Alzheimer’s disease: biomarker profiling approach |
title_sort |
central nervous system and peripheral inflammatory processes in alzheimer’s disease: biomarker profiling approach |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neurology |
issn |
1664-2295 |
publishDate |
2015-08-01 |
description |
Brain inflammation is one of the hallmarks of Alzheimer disease (AD) and a current trend is that inflammatory mediators, particularly cytokines and chemokines, may represent valuable biomarkers for early screening and diagnosis of the disease. Various studies have reported differences in serum level of cytokines, chemokines, and growth factors in patients with MCI (Mild Cognitive Impairment) or AD. However, data were often inconsistent and the exact function of inflammation in neurodegeneration is still a matter of debate.In the present work, we measured the expression of 120 biomarkers (corresponding to cytokines, chemokines, growth factors, and related signalling proteins) in the serum of 49 patients with the following diagnosis distribution: 15 controls, 14 AD and 20 MCI. In addition, we performed the same analysis in the cerebrospinal fluid (CSF) of 20 of these patients (10 AD and 10 controls). Among the biomarkers tested, none showed significant changes in the serum, but 13 were significantly modified in the CSF of AD patients. Interestingly, all of these biomarkers were implicated in neurogenesis or neural stem cells migration and differentiation. In a second part of the study, 10 of these putative biomarkers (plus 4 additional) were quantified using quantitative multiplex ELISA methods in the CSF and the serum of an enlarged cohort composed of 31 AD and 24 control patients.Our results confirm the potential diagnosis interest of previously published blood biomarkers, and proposes new ones (such as IL-8 and TNFR-I). Further studies will be needed to validate these biomarkers which could be used alone, combined, or in association with the classical amyloid and tau biomarkers. |
topic |
Alzheimer Disease Cerebrospinal Fluid Inflammation Serum biomarkers |
url |
http://journal.frontiersin.org/Journal/10.3389/fneur.2015.00181/full |
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