Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows
Ketosis is a metabolic disorder closely associated with liver lipidosis. Numerous tests have been developed to detect hepatic dysfunction in dairy cows. Bromosulfophthalein (BSP) clearance is established as a sensitive index of hepatic function. The objective of this study was to examine the differe...
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Ss.Cyril and Methodius University, Skopje
2013-09-01
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doaj-dfe7cb9f487b4d2c850e873ed550558b2020-11-25T01:17:16ZengSs.Cyril and Methodius University, SkopjeMacedonian Veterinary Review 1409-76211857-74152013-09-013628589Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cowsDanijela KirovskiŽeljko SladojevićHorea ŠamancKetosis is a metabolic disorder closely associated with liver lipidosis. Numerous tests have been developed to detect hepatic dysfunction in dairy cows. Bromosulfophthalein (BSP) clearance is established as a sensitive index of hepatic function. The objective of this study was to examine the difference of biliary excretion of BSP between ketotic and healthy Holstein cows and to correlate this excretion with other indicators of liver dysfunction. Twenty puerperal Holstein cows divided in two groups (10 cows each) were involved in the study. The first group included healthy and the second group ketotic cows. Blood samples were taken 10 days after parturition. Concentrations of total protein, albumin, total bilirubin, Ca, P, total lipids, urea and glucose were determined. Immediately after blood sampling, BSP test was performed. Blood samples were taken 5 and 45 minutes after injection, and the percentage of retained pigment in the sample obtained at minute 45 was calculated. Blood albumin and glucose concentrations were significantly higher in healthy then ketotic cows. Total bilirubin concentration was significantly higher in ketotic than healthy cows. BSP excretion was significantly higher in ketotic compared to healthy cows. There was a significant positive correlation between BSP values and total bilirubin concentrartions in both healthy and ketotic cows and a significant negative correlation between BSP values and glucose concentrartions in both healthy and ketotic cows. In conclusion, biliary clearance of BSP may be used as a reliable method for the detection of hepatic dysfunction associated with clinical symptoms of ketosis in dairy cows.http://www.macvetrev.mk/2013-2/Mac%20Vet%20Rev%202013;%2036%20%282%29;%2085-89.pdfHolstein cowsBSP testketosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Danijela Kirovski Željko Sladojević Horea Šamanc |
spellingShingle |
Danijela Kirovski Željko Sladojević Horea Šamanc Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows Macedonian Veterinary Review Holstein cows BSP test ketosis |
author_facet |
Danijela Kirovski Željko Sladojević Horea Šamanc |
author_sort |
Danijela Kirovski |
title |
Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows |
title_short |
Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows |
title_full |
Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows |
title_fullStr |
Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows |
title_full_unstemmed |
Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows |
title_sort |
biliary clearance of bromosulfophthalein in healthy and ketotic holstein cows |
publisher |
Ss.Cyril and Methodius University, Skopje |
series |
Macedonian Veterinary Review |
issn |
1409-7621 1857-7415 |
publishDate |
2013-09-01 |
description |
Ketosis is a metabolic disorder closely associated with liver lipidosis. Numerous tests have been developed to detect hepatic dysfunction in dairy cows. Bromosulfophthalein (BSP) clearance is established as a sensitive index of hepatic function. The objective of this study was to examine the difference of biliary excretion of BSP between ketotic and healthy Holstein cows and to correlate this excretion with other indicators of liver dysfunction. Twenty puerperal Holstein cows divided in two groups (10 cows each) were involved in the study. The first group included healthy and the second group ketotic cows. Blood samples were taken 10 days after parturition. Concentrations of total protein, albumin, total bilirubin, Ca, P, total lipids, urea and glucose were determined. Immediately after blood sampling, BSP test was performed. Blood samples were taken 5 and 45 minutes after injection, and the percentage of retained pigment in the sample obtained at minute 45 was calculated. Blood albumin and glucose concentrations were significantly higher in healthy then ketotic cows. Total bilirubin concentration was significantly higher in ketotic than healthy cows. BSP excretion was significantly higher in ketotic compared to healthy cows. There was a significant positive correlation between BSP values and total bilirubin concentrartions in both healthy and ketotic cows and a significant negative correlation between BSP values and glucose concentrartions in both healthy and ketotic cows. In conclusion, biliary clearance of BSP may be used as a reliable method for the detection of hepatic dysfunction associated with clinical symptoms of ketosis in dairy cows. |
topic |
Holstein cows BSP test ketosis |
url |
http://www.macvetrev.mk/2013-2/Mac%20Vet%20Rev%202013;%2036%20%282%29;%2085-89.pdf |
work_keys_str_mv |
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