An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice
Thymosin β4 (Tβ4) treatment was known to show the potential therapeutic effects on diabetic complications. This study was performed to determine if Tβ4 expression is changed in both serum and tissues under diabetic conditions and can be a serum biomarker. Type 1 diabetic mice were induced in C57/BL6...
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doaj-dfea075564c84abf969ed703c053eb5b2020-11-25T02:49:56ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/34215683421568An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic MiceKyung Sook Cho0Dong-Jin Kim1Bomee Shim2Jung Yeon Kim3Jun Mo Kang4Seon Hwa Park5Sang-Ho Lee6Hyung-In Yang7Kyoung Soo Kim8Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, Republic of KoreaDepartment of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Pathology, Inje University Sanggye Paik Hospital, Nowon-gu, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, Republic of KoreaDivision of Rheumatology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Gangdong-gu, Seoul, Republic of KoreaDepartment of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Republic of KoreaThymosin β4 (Tβ4) treatment was known to show the potential therapeutic effects on diabetic complications. This study was performed to determine if Tβ4 expression is changed in both serum and tissues under diabetic conditions and can be a serum biomarker. Type 1 diabetic mice were induced in C57/BL6J mice by intraperitoneal injection of streptozotocin (STZ) at a dose of 50 mg/kg body weight. The mice were sacrificed at 16 weeks after STZ injection. Tissues and plasmas were obtained to determine the expression levels of Tβ4 using ELISA, real time RT-PCR, and immunohistochemistry. The average serum glucose level was increased to approximately 400 mg/dL beginning 2 weeks after the five injections of STZ and lasting for at least 13 weeks until sacrifice. The plasma and tissue levels of Tβ4 in the age-matched control mice were not significantly different from those of the diabetic mice. In conclusion, the Tβ4 expression level in the plasmas and tissues of diabetic mice was not affected by diabetic conditions. It indirectly suggests that the therapeutic effect of Tβ4 on diabetic complications is due to its regenerative effects on damaged tissue but not to the changed expression level of Tβ4 in plasma and tissues of diabetes.http://dx.doi.org/10.1155/2018/3421568 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyung Sook Cho Dong-Jin Kim Bomee Shim Jung Yeon Kim Jun Mo Kang Seon Hwa Park Sang-Ho Lee Hyung-In Yang Kyoung Soo Kim |
spellingShingle |
Kyung Sook Cho Dong-Jin Kim Bomee Shim Jung Yeon Kim Jun Mo Kang Seon Hwa Park Sang-Ho Lee Hyung-In Yang Kyoung Soo Kim An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice BioMed Research International |
author_facet |
Kyung Sook Cho Dong-Jin Kim Bomee Shim Jung Yeon Kim Jun Mo Kang Seon Hwa Park Sang-Ho Lee Hyung-In Yang Kyoung Soo Kim |
author_sort |
Kyung Sook Cho |
title |
An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice |
title_short |
An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice |
title_full |
An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice |
title_fullStr |
An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice |
title_full_unstemmed |
An Investigation on the Therapeutic Effect of Thymosin β4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice |
title_sort |
investigation on the therapeutic effect of thymosin β4 and its expression levels in streptozotocin-induced diabetic mice |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
Thymosin β4 (Tβ4) treatment was known to show the potential therapeutic effects on diabetic complications. This study was performed to determine if Tβ4 expression is changed in both serum and tissues under diabetic conditions and can be a serum biomarker. Type 1 diabetic mice were induced in C57/BL6J mice by intraperitoneal injection of streptozotocin (STZ) at a dose of 50 mg/kg body weight. The mice were sacrificed at 16 weeks after STZ injection. Tissues and plasmas were obtained to determine the expression levels of Tβ4 using ELISA, real time RT-PCR, and immunohistochemistry. The average serum glucose level was increased to approximately 400 mg/dL beginning 2 weeks after the five injections of STZ and lasting for at least 13 weeks until sacrifice. The plasma and tissue levels of Tβ4 in the age-matched control mice were not significantly different from those of the diabetic mice. In conclusion, the Tβ4 expression level in the plasmas and tissues of diabetic mice was not affected by diabetic conditions. It indirectly suggests that the therapeutic effect of Tβ4 on diabetic complications is due to its regenerative effects on damaged tissue but not to the changed expression level of Tβ4 in plasma and tissues of diabetes. |
url |
http://dx.doi.org/10.1155/2018/3421568 |
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