Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts
Background: The roots of <i>Codonopsis pilosula</i> (Franch.) Nannf. have been used in traditional Chinese medicine for treating cardiovascular disease. In the current study, we aimed to discover herbal extracts from <i>C. pilosula</i> that are capable of improving cardiac fu...
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doaj-dff1bf20656a49bf988786335eb0b17f2021-05-31T23:15:34ZengMDPI AGLife2075-17292021-05-011142242210.3390/life11050422Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat HeartsJieh-Neng Wang0Chung-Dann Kan1Lain-Tze Lee2Lynn L. H. Huang3Ya-Li Hsiao4Allen H. Chang5Wanchun Liu6Cheng Lin7Chou-Wen Lin8Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70401, TaiwanDepartment of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70401, TaiwanHerbal Medical Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu 31057, TaiwanDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 70401, TaiwanDepartment of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70401, TaiwanDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 70401, TaiwanHerbal Medical Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu 31057, TaiwanHerbal Medical Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu 31057, TaiwanHerbal Medical Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu 31057, TaiwanBackground: The roots of <i>Codonopsis pilosula</i> (Franch.) Nannf. have been used in traditional Chinese medicine for treating cardiovascular disease. In the current study, we aimed to discover herbal extracts from <i>C. pilosula</i> that are capable of improving cardiac function of infarcted hearts to develop a potential therapeutic approach. Methods: A mouse embryonic stem (ES) cell-based model with an enhanced green fluorescent protein (eGFP) reporter driven by a cardiomyocyte-specific promoter, the α-myosin heavy chain, was constructed to evaluate the cardiogenic activity of herbal extracts. Then, herbal extracts from <i>C. pilosula</i> with cardiogenic activity based on an increase in eGFP expression during ES cell differentiation were further tested in a rat myocardial infarction model with left anterior descending artery (LAD) ligation. Cardiac function assessments were performed using echocardiography, 1, 3, and 6 weeks post LAD ligation. Results: The herbal extract 417W from <i>C. pilosula</i> was capable of enhancing cardiogenic differentiation in mouse ES cells in vitro. Echocardiography results in the LAD-ligated rat model revealed significant improvements in the infarcted hearts at least 6 weeks after 417W treatment that were determined based on left ventricle fractional shortening (FS), fractional area contraction (FAC), and ejection fraction (EF). Conclusions: The herbal extract 417W can enhance the cardiogenic differentiation of ES cells and improve the cardiac function of infarcted hearts.https://www.mdpi.com/2075-1729/11/5/422herbal extractscardiogenesiscardiomyocytesmyocardial infarction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jieh-Neng Wang Chung-Dann Kan Lain-Tze Lee Lynn L. H. Huang Ya-Li Hsiao Allen H. Chang Wanchun Liu Cheng Lin Chou-Wen Lin |
spellingShingle |
Jieh-Neng Wang Chung-Dann Kan Lain-Tze Lee Lynn L. H. Huang Ya-Li Hsiao Allen H. Chang Wanchun Liu Cheng Lin Chou-Wen Lin Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts Life herbal extracts cardiogenesis cardiomyocytes myocardial infarction |
author_facet |
Jieh-Neng Wang Chung-Dann Kan Lain-Tze Lee Lynn L. H. Huang Ya-Li Hsiao Allen H. Chang Wanchun Liu Cheng Lin Chou-Wen Lin |
author_sort |
Jieh-Neng Wang |
title |
Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts |
title_short |
Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts |
title_full |
Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts |
title_fullStr |
Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts |
title_full_unstemmed |
Herbal Extract from <i>Codonopsis pilosula</i> (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts |
title_sort |
herbal extract from <i>codonopsis pilosula</i> (franch.) nannf. enhances cardiogenic differentiation and improves the function of infarcted rat hearts |
publisher |
MDPI AG |
series |
Life |
issn |
2075-1729 |
publishDate |
2021-05-01 |
description |
Background: The roots of <i>Codonopsis pilosula</i> (Franch.) Nannf. have been used in traditional Chinese medicine for treating cardiovascular disease. In the current study, we aimed to discover herbal extracts from <i>C. pilosula</i> that are capable of improving cardiac function of infarcted hearts to develop a potential therapeutic approach. Methods: A mouse embryonic stem (ES) cell-based model with an enhanced green fluorescent protein (eGFP) reporter driven by a cardiomyocyte-specific promoter, the α-myosin heavy chain, was constructed to evaluate the cardiogenic activity of herbal extracts. Then, herbal extracts from <i>C. pilosula</i> with cardiogenic activity based on an increase in eGFP expression during ES cell differentiation were further tested in a rat myocardial infarction model with left anterior descending artery (LAD) ligation. Cardiac function assessments were performed using echocardiography, 1, 3, and 6 weeks post LAD ligation. Results: The herbal extract 417W from <i>C. pilosula</i> was capable of enhancing cardiogenic differentiation in mouse ES cells in vitro. Echocardiography results in the LAD-ligated rat model revealed significant improvements in the infarcted hearts at least 6 weeks after 417W treatment that were determined based on left ventricle fractional shortening (FS), fractional area contraction (FAC), and ejection fraction (EF). Conclusions: The herbal extract 417W can enhance the cardiogenic differentiation of ES cells and improve the cardiac function of infarcted hearts. |
topic |
herbal extracts cardiogenesis cardiomyocytes myocardial infarction |
url |
https://www.mdpi.com/2075-1729/11/5/422 |
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