Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.)
Study of genes involved in flower formation could help to increase our understanding of floral organ identity mechanisms. In this research, the expression pattern of four MADS- box gene family including <em>RaA2, RaB17, RaG24</em> and <em>RaK17</em> isolated from the dioeciou...
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Shahid Bahonar University of Kerman
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doaj-dff791e344b646f0b7e3b0e2e157b82a2020-11-25T03:20:08ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002009-06-011111810.22103/jab.2009.11471147Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.)Ali Mohammad Shakib0Charls Ainsworth1پژوهشکده بیوتکنولوژی کشاورزی ایران- کرجآزمایشگاه بیولوژی ملکولی گیاهی، ایمپریال کالج، انگلستانStudy of genes involved in flower formation could help to increase our understanding of floral organ identity mechanisms. In this research, the expression pattern of four MADS- box gene family including <em>RaA2, RaB17, RaG24</em> and <em>RaK17</em> isolated from the dioecious plant <em>Rumex acetosa</em> was studied. RNA extracted from different tissues (inflorescence, stem, leaf and root) of male and female plants, was used for Northern blot analysis. <em>RaA2</em> and <em>RaB17</em> genes are expressed in reproductive organs but <em>RaG24</em> and <em>Ra K17</em> genes were shown to be expressed in both vegetative and reproductive organs. Alignment of their deduced proteins with other known MADS box proteins showed that they are belongs to distinct subfamily groups. The proteins of <em>RaA2</em> and <em>RaB17</em> genes showed the highest similarity to MADS proteins from <em>Malus domestica</em> and CMB1 from <em>Diantus caryophyllus</em>, respectively. The proteins of <em>RaG24</em> and <em>Ra K17</em> genes have the highest similarity to TDR3 protein from <em>L</em><em>ycopersicon esculentum</em> and MADS5 protein from <em>Betula pendula</em> and POTM1 protein from <em>Solanum tuberosum</em> which are expressed in both vegetative and reproductive phases. In Southern blot analysis, there is a difference in hybridisation pattern between male and female. The simple pattern of hybridisation indicated that there is a single copy and small gene family of each gene in the genome.https://jab.uk.ac.ir/article_1147_cfd719a2801ceb3e76fa6b13206cf9e1.pdfmads- boxrumex acetosa ldioecious plantgene expression |
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DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Ali Mohammad Shakib Charls Ainsworth |
spellingShingle |
Ali Mohammad Shakib Charls Ainsworth Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.) مجله بیوتکنولوژی کشاورزی mads- box rumex acetosa l dioecious plant gene expression |
author_facet |
Ali Mohammad Shakib Charls Ainsworth |
author_sort |
Ali Mohammad Shakib |
title |
Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.) |
title_short |
Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.) |
title_full |
Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.) |
title_fullStr |
Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.) |
title_full_unstemmed |
Expression study of four gene clones belonging to MADS- box family in the dioecious plant sorrel (Rumex acetosa L.) |
title_sort |
expression study of four gene clones belonging to mads- box family in the dioecious plant sorrel (rumex acetosa l.) |
publisher |
Shahid Bahonar University of Kerman |
series |
مجله بیوتکنولوژی کشاورزی |
issn |
2228-6705 2228-6500 |
publishDate |
2009-06-01 |
description |
Study of genes involved in flower formation could help to increase our understanding of floral organ identity mechanisms. In this research, the expression pattern of four MADS- box gene family including <em>RaA2, RaB17, RaG24</em> and <em>RaK17</em> isolated from the dioecious plant <em>Rumex acetosa</em> was studied. RNA extracted from different tissues (inflorescence, stem, leaf and root) of male and female plants, was used for Northern blot analysis. <em>RaA2</em> and <em>RaB17</em> genes are expressed in reproductive organs but <em>RaG24</em> and <em>Ra K17</em> genes were shown to be expressed in both vegetative and reproductive organs. Alignment of their deduced proteins with other known MADS box proteins showed that they are belongs to distinct subfamily groups. The proteins of <em>RaA2</em> and <em>RaB17</em> genes showed the highest similarity to MADS proteins from <em>Malus domestica</em> and CMB1 from <em>Diantus caryophyllus</em>, respectively. The proteins of <em>RaG24</em> and <em>Ra K17</em> genes have the highest similarity to TDR3 protein from <em>L</em><em>ycopersicon esculentum</em> and MADS5 protein from <em>Betula pendula</em> and POTM1 protein from <em>Solanum tuberosum</em> which are expressed in both vegetative and reproductive phases. In Southern blot analysis, there is a difference in hybridisation pattern between male and female. The simple pattern of hybridisation indicated that there is a single copy and small gene family of each gene in the genome. |
topic |
mads- box rumex acetosa l dioecious plant gene expression |
url |
https://jab.uk.ac.ir/article_1147_cfd719a2801ceb3e76fa6b13206cf9e1.pdf |
work_keys_str_mv |
AT alimohammadshakib expressionstudyoffourgeneclonesbelongingtomadsboxfamilyinthedioeciousplantsorrelrumexacetosal AT charlsainsworth expressionstudyoffourgeneclonesbelongingtomadsboxfamilyinthedioeciousplantsorrelrumexacetosal |
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