Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers

• Main Authors: Ikuma Kasuga, Peter D. Paré, Andrew J. Sandford
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Genética 2006-01-01
• Series: Genetics and Molecular Biology
• Subjects: HLA-A1; PCR; allele group-specific primers
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003

We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods.