Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation
Summary: Separation of highly enriched extracellular vesicles (EVs) fractions from milk is desirable for quantification, cargo analysis, functional characterization, and investigation as delivery vehicles for nutrients and/or therapeutics. However, a rigorous, reproducible protocol is lacking. This...
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2021-12-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672100527X |
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doaj-e007d4af4e194a8a80b548a47bb8b6c12021-09-17T04:37:56ZengElsevierSTAR Protocols2666-16672021-12-0124100821Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugationAnindya Mukhopadhya0Jessie Santoro1Lorraine O’Driscoll2School of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences Institute, Trinity College Dublin and Trinity St. James’s Cancer Institute, Dublin 2, Ireland; Corresponding authorSchool of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences Institute, Trinity College Dublin and Trinity St. James’s Cancer Institute, Dublin 2, IrelandSchool of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences Institute, Trinity College Dublin and Trinity St. James’s Cancer Institute, Dublin 2, IrelandSummary: Separation of highly enriched extracellular vesicles (EVs) fractions from milk is desirable for quantification, cargo analysis, functional characterization, and investigation as delivery vehicles for nutrients and/or therapeutics. However, a rigorous, reproducible protocol is lacking. This protocol considers a crucial aspect typically overlooked, i.e., that caseins are of similar size to, but more abundant than, EVs in milk. Our protocol combines acid pre-treatment and gradient ultracentrifugation, producing EV-enriched fractions suitable for downstream orthogonal characterization approaches.For complete details on the use and execution of this protocol, please refer to Mukhopadhya et al. (2021).http://www.sciencedirect.com/science/article/pii/S266616672100527XHealth SciencesMolecular BiologyProtein BiochemistryProtein expression and purificationBiotechnology and bioengineering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anindya Mukhopadhya Jessie Santoro Lorraine O’Driscoll |
spellingShingle |
Anindya Mukhopadhya Jessie Santoro Lorraine O’Driscoll Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation STAR Protocols Health Sciences Molecular Biology Protein Biochemistry Protein expression and purification Biotechnology and bioengineering |
author_facet |
Anindya Mukhopadhya Jessie Santoro Lorraine O’Driscoll |
author_sort |
Anindya Mukhopadhya |
title |
Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation |
title_short |
Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation |
title_full |
Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation |
title_fullStr |
Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation |
title_full_unstemmed |
Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation |
title_sort |
extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation |
publisher |
Elsevier |
series |
STAR Protocols |
issn |
2666-1667 |
publishDate |
2021-12-01 |
description |
Summary: Separation of highly enriched extracellular vesicles (EVs) fractions from milk is desirable for quantification, cargo analysis, functional characterization, and investigation as delivery vehicles for nutrients and/or therapeutics. However, a rigorous, reproducible protocol is lacking. This protocol considers a crucial aspect typically overlooked, i.e., that caseins are of similar size to, but more abundant than, EVs in milk. Our protocol combines acid pre-treatment and gradient ultracentrifugation, producing EV-enriched fractions suitable for downstream orthogonal characterization approaches.For complete details on the use and execution of this protocol, please refer to Mukhopadhya et al. (2021). |
topic |
Health Sciences Molecular Biology Protein Biochemistry Protein expression and purification Biotechnology and bioengineering |
url |
http://www.sciencedirect.com/science/article/pii/S266616672100527X |
work_keys_str_mv |
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