Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected
Abstract Background The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red...
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doaj-e02bf7d25f914740939c8340dd1ce18a2020-11-25T03:04:13ZengBMCBMC Veterinary Research1746-61482020-06-0116111510.1186/s12917-020-02418-zAdministration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affectedCristian Porcu0Francesca D. Sotgiu1Valeria Pasciu2Maria Grazia Cappai3Alicia Barbero-Fernández4Antonio Gonzalez-Bulnes5Maria Dattena6Marilia Gallus7Giovanni Molle8Fiammetta Berlinguer9Department of Veterinary Medicine, University of SassariDepartment of Veterinary Medicine, University of SassariDepartment of Veterinary Medicine, University of SassariDepartment of Veterinary Medicine, University of SassariUniversidad Alfonso X el Sabio, Campus de Villanueva de la CañadaComparative Physiology Group, SGIT-INIAAGRIS SardegnaAGRIS SardegnaAGRIS SardegnaDepartment of Veterinary Medicine, University of SassariAbstract Background The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe’s ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG). Results Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v). Treatments were formulated to provide 100, 75, 50 and 25% of the amount of energy supplied in previous experiments. Obtained results showed that the formulations G75 and M75 (22.5 and 18.2% on DM basis, respectively) induce metabolic changes comparable to those induced by M100. The latter dose has been already evaluated for its effects on sheep metabolism and reproductive performance. However, with these high doses, plasma osmolality increased significantly, and RBC indices showed significant alterations. The low dose groups (G25 and M25, 8.6 and 6.9% on DM basis, respectively) did not show any alterations in plasma osmolality and RBC indices, but the metabolic milieu differed markedly from that of M100. Between the medium dose groups, M50 (12.9% on DM basis) showed a more comparable milieu to M100 than G50 (15.9% on DM basis) and no RBC alterations. Therefore, M75, G75 and M50 doses were tested for their effect on ovarian functions and proved to be equally effective as eCG. Conclusion The results of the present study evidenced an alteration of RBC indices, and possibly of their functions, as a side effect of glycerol administration at high doses in the diet of ewes. Therefore, protocols foreseeing the administration of glycerol should be tested for their effects on RBC indices and functions. In general terms, the medium dose of the glucogenic mixture (12.9% of dietary DM on offer) should be preferred.http://link.springer.com/article/10.1186/s12917-020-02418-zPropylene glycolOvaryInsulinGlucoseNEFAUrea |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristian Porcu Francesca D. Sotgiu Valeria Pasciu Maria Grazia Cappai Alicia Barbero-Fernández Antonio Gonzalez-Bulnes Maria Dattena Marilia Gallus Giovanni Molle Fiammetta Berlinguer |
spellingShingle |
Cristian Porcu Francesca D. Sotgiu Valeria Pasciu Maria Grazia Cappai Alicia Barbero-Fernández Antonio Gonzalez-Bulnes Maria Dattena Marilia Gallus Giovanni Molle Fiammetta Berlinguer Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected BMC Veterinary Research Propylene glycol Ovary Insulin Glucose NEFA Urea |
author_facet |
Cristian Porcu Francesca D. Sotgiu Valeria Pasciu Maria Grazia Cappai Alicia Barbero-Fernández Antonio Gonzalez-Bulnes Maria Dattena Marilia Gallus Giovanni Molle Fiammetta Berlinguer |
author_sort |
Cristian Porcu |
title |
Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected |
title_short |
Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected |
title_full |
Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected |
title_fullStr |
Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected |
title_full_unstemmed |
Administration of glycerol-based formulations in sheep results in similar ovulation rate to eCG but red blood cell indices may be affected |
title_sort |
administration of glycerol-based formulations in sheep results in similar ovulation rate to ecg but red blood cell indices may be affected |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-06-01 |
description |
Abstract Background The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe’s ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG). Results Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v). Treatments were formulated to provide 100, 75, 50 and 25% of the amount of energy supplied in previous experiments. Obtained results showed that the formulations G75 and M75 (22.5 and 18.2% on DM basis, respectively) induce metabolic changes comparable to those induced by M100. The latter dose has been already evaluated for its effects on sheep metabolism and reproductive performance. However, with these high doses, plasma osmolality increased significantly, and RBC indices showed significant alterations. The low dose groups (G25 and M25, 8.6 and 6.9% on DM basis, respectively) did not show any alterations in plasma osmolality and RBC indices, but the metabolic milieu differed markedly from that of M100. Between the medium dose groups, M50 (12.9% on DM basis) showed a more comparable milieu to M100 than G50 (15.9% on DM basis) and no RBC alterations. Therefore, M75, G75 and M50 doses were tested for their effect on ovarian functions and proved to be equally effective as eCG. Conclusion The results of the present study evidenced an alteration of RBC indices, and possibly of their functions, as a side effect of glycerol administration at high doses in the diet of ewes. Therefore, protocols foreseeing the administration of glycerol should be tested for their effects on RBC indices and functions. In general terms, the medium dose of the glucogenic mixture (12.9% of dietary DM on offer) should be preferred. |
topic |
Propylene glycol Ovary Insulin Glucose NEFA Urea |
url |
http://link.springer.com/article/10.1186/s12917-020-02418-z |
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