Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes
In vitro follicle growth (IVFG) is an emerging fertility preservation technique, which can obtain fertilizable oocytes from an in vitro culture system in female. This study aimed to compare efficiency of the most widely used two-dimensional follicle culture methods [with or without oil layer (O+ or...
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doaj-e04734125cf54a439e30833843f843772020-11-24T22:28:16ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/79070927907092Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured OocytesJaewang Lee0Eun Jung Kim1Hyun Sun Kong2Hye Won Youm3Seul Ki Kim4Jung Ryeol Lee5Chang Suk Suh6Seok Hyun Kim7Department of Biomedical Laboratory Science, Eulji University, Seongnam, Gyeonggi-do, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of KoreaDepartment of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of KoreaIn vitro follicle growth (IVFG) is an emerging fertility preservation technique, which can obtain fertilizable oocytes from an in vitro culture system in female. This study aimed to compare efficiency of the most widely used two-dimensional follicle culture methods [with or without oil layer (O+ or O− group)]. Preantral follicles were isolated from mice and randomly assigned. Follicles were cultured for 10 days and cumulus-oocyte complexes harvested 16–18 hours after hCG treatment. Follicle and oocyte growth, hormones in spent medium, meiotic spindle localization, expression of reactive oxygen species (ROS), mitochondrial activity, and gene expression were evaluated. In follicle growth, survival, pseudoantral cavity formation, ovulation, and oocyte maturation were also significantly higher in O+ group than O− group. Hormone production was significantly higher in follicles cultured in O+ than O−. There were no significant differences in mRNA expression related to development. On the other hand, the level of ROS was increased while the mitochondrial activity of in vitro grown matured oocyte was less than in vivo matured oocytes. In conclusion, follicle culture with O+ group appears to be superior to the culture in O− group in terms of follicle growth, development, oocyte growth, maturation, and microorganelles in oocyte.http://dx.doi.org/10.1155/2018/7907092 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jaewang Lee Eun Jung Kim Hyun Sun Kong Hye Won Youm Seul Ki Kim Jung Ryeol Lee Chang Suk Suh Seok Hyun Kim |
spellingShingle |
Jaewang Lee Eun Jung Kim Hyun Sun Kong Hye Won Youm Seul Ki Kim Jung Ryeol Lee Chang Suk Suh Seok Hyun Kim Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes BioMed Research International |
author_facet |
Jaewang Lee Eun Jung Kim Hyun Sun Kong Hye Won Youm Seul Ki Kim Jung Ryeol Lee Chang Suk Suh Seok Hyun Kim |
author_sort |
Jaewang Lee |
title |
Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes |
title_short |
Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes |
title_full |
Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes |
title_fullStr |
Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes |
title_full_unstemmed |
Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes |
title_sort |
comparison of the oocyte quality derived from two-dimensional follicle culture methods and developmental competence of in vitro grown and matured oocytes |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
In vitro follicle growth (IVFG) is an emerging fertility preservation technique, which can obtain fertilizable oocytes from an in vitro culture system in female. This study aimed to compare efficiency of the most widely used two-dimensional follicle culture methods [with or without oil layer (O+ or O− group)]. Preantral follicles were isolated from mice and randomly assigned. Follicles were cultured for 10 days and cumulus-oocyte complexes harvested 16–18 hours after hCG treatment. Follicle and oocyte growth, hormones in spent medium, meiotic spindle localization, expression of reactive oxygen species (ROS), mitochondrial activity, and gene expression were evaluated. In follicle growth, survival, pseudoantral cavity formation, ovulation, and oocyte maturation were also significantly higher in O+ group than O− group. Hormone production was significantly higher in follicles cultured in O+ than O−. There were no significant differences in mRNA expression related to development. On the other hand, the level of ROS was increased while the mitochondrial activity of in vitro grown matured oocyte was less than in vivo matured oocytes. In conclusion, follicle culture with O+ group appears to be superior to the culture in O− group in terms of follicle growth, development, oocyte growth, maturation, and microorganelles in oocyte. |
url |
http://dx.doi.org/10.1155/2018/7907092 |
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