Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1
Natural transformation and viral-mediated transduction are the main avenues of horizontal gene transfer in Firmicutes. Bacillus subtilis SPP1 is a generalized transducing bacteriophage. Using this lytic phage as a model, we have analyzed how viral replication and recombination systems contribute to...
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doaj-e05bbe27cb404ea2bce12f11156388212020-11-24T22:34:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01816295854Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1Ana Valero-Rello0Ana Valero-Rello1María López-Sanz2Alvaro Quevedo-Olmos3Alexei Sorokin4Silvia Ayora5Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones CientíficasMadrid, SpainMicalis Institute, INRA, AgroParisTech, Universite Paris-SaclayJouy-en-Josas, FranceDepartment of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones CientíficasMadrid, SpainDepartment of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones CientíficasMadrid, SpainMicalis Institute, INRA, AgroParisTech, Universite Paris-SaclayJouy-en-Josas, FranceDepartment of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones CientíficasMadrid, SpainNatural transformation and viral-mediated transduction are the main avenues of horizontal gene transfer in Firmicutes. Bacillus subtilis SPP1 is a generalized transducing bacteriophage. Using this lytic phage as a model, we have analyzed how viral replication and recombination systems contribute to the transfer of plasmid-borne antibiotic resistances. Phage SPP1 DNA replication relies on essential phage-encoded replisome organizer (G38P), helicase loader (G39P), hexameric replicative helicase (G40P), recombinase (G35P) and in less extent on the partially dispensable 5′→3′ exonuclease (G34.1P), the single-stranded DNA binding protein (G36P) and the Holliday junction resolvase (G44P). Correspondingly, the accumulation of linear concatemeric plasmid DNA, and the formation of transducing particles were blocked in the absence of G35P, G38P, G39P, and G40P, greatly reduced in the G34.1P, G36P mutants, and slightly reduced in G44P mutants. In contrast, establishment of injected linear plasmid DNA in the recipient host was independent of viral-encoded functions. DNA homology between SPP1 and the plasmid, rather than a viral packaging signal, enhanced the accumulation of packagable plasmid DNA. The transfer efficiency was also dependent on plasmid copy number, and rolling-circle plasmids were encapsidated at higher frequencies than theta-type replicating plasmids.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01816/fullhorizontal gene transferplasmid transductionSPP1bacteriophagesantibiotic resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Valero-Rello Ana Valero-Rello María López-Sanz Alvaro Quevedo-Olmos Alexei Sorokin Silvia Ayora |
spellingShingle |
Ana Valero-Rello Ana Valero-Rello María López-Sanz Alvaro Quevedo-Olmos Alexei Sorokin Silvia Ayora Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1 Frontiers in Microbiology horizontal gene transfer plasmid transduction SPP1 bacteriophages antibiotic resistance |
author_facet |
Ana Valero-Rello Ana Valero-Rello María López-Sanz Alvaro Quevedo-Olmos Alexei Sorokin Silvia Ayora |
author_sort |
Ana Valero-Rello |
title |
Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1 |
title_short |
Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1 |
title_full |
Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1 |
title_fullStr |
Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1 |
title_full_unstemmed |
Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1 |
title_sort |
molecular mechanisms that contribute to horizontal transfer of plasmids by the bacteriophage spp1 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-09-01 |
description |
Natural transformation and viral-mediated transduction are the main avenues of horizontal gene transfer in Firmicutes. Bacillus subtilis SPP1 is a generalized transducing bacteriophage. Using this lytic phage as a model, we have analyzed how viral replication and recombination systems contribute to the transfer of plasmid-borne antibiotic resistances. Phage SPP1 DNA replication relies on essential phage-encoded replisome organizer (G38P), helicase loader (G39P), hexameric replicative helicase (G40P), recombinase (G35P) and in less extent on the partially dispensable 5′→3′ exonuclease (G34.1P), the single-stranded DNA binding protein (G36P) and the Holliday junction resolvase (G44P). Correspondingly, the accumulation of linear concatemeric plasmid DNA, and the formation of transducing particles were blocked in the absence of G35P, G38P, G39P, and G40P, greatly reduced in the G34.1P, G36P mutants, and slightly reduced in G44P mutants. In contrast, establishment of injected linear plasmid DNA in the recipient host was independent of viral-encoded functions. DNA homology between SPP1 and the plasmid, rather than a viral packaging signal, enhanced the accumulation of packagable plasmid DNA. The transfer efficiency was also dependent on plasmid copy number, and rolling-circle plasmids were encapsidated at higher frequencies than theta-type replicating plasmids. |
topic |
horizontal gene transfer plasmid transduction SPP1 bacteriophages antibiotic resistance |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01816/full |
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