Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins

Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins

Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B. anthracis protective antigen ion channels th...

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Bibliographic Details
Main Authors: Vitalii Silin, John J. Kasianowicz, Ariel Michelman-Ribeiro, Rekha G. Panchal, Sina Bavari, Joseph W. F. Robertson
Format: Article
Language:English
Published: MDPI AG 2016-06-01
Series:Membranes
Subjects:
anthrax
protective antigen
lethal factor
edema factor
therapeutic agents
screening
tethered bilayer membrane
biochip
Online Access:http://www.mdpi.com/2077-0375/6/3/36
Description
Summary:Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B. anthracis protective antigen ion channels that are both the recognition element and electrochemical transducer. We characterize the sensor and its properties with electrochemical impedance spectroscopy and surface plasmon resonance. The sensor shows a sensitivity similar to ELISA and can also be used to rapidly screen for molecules that bind to the toxins and potentially inhibit their lethal effects.
ISSN:2077-0375

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