Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma
Objective: The objective of this study is to investigate the expression of Vascular cell adhesion molecule-1 (VCAM-1) and very late appearing antigen-4 (VLA-4) cytokines in MM (multiple Myeloma). Method: Forty patients with MM are selected as the experimental group and 30 healthy persons as the cont...
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doaj-e06a7085617d480ea1de4c001c7c52e92020-11-25T03:03:18ZengElsevierSaudi Journal of Biological Sciences1319-562X2020-06-0127616741678Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myelomaPan Hao0Chunli Zhang1Rongfu Wang2Ping Yan3Ruchen Peng4Department of Nuclear Medicine, LuHe Hospital, Capital Medical University, Beijing City 101149, ChinaDepartment of Nuclear Medicine, Peaking University First Hospital, Beijing City 100011, ChinaDepartment of Nuclear Medicine, Peaking University First Hospital, Beijing City 100011, ChinaDepartment of Nuclear Medicine, Peaking University First Hospital, Beijing City 100011, ChinaDepartment of Medical Imaging Center Nuclear Medicine, LuHe Hospital, Capital Medical University, Beijing City 101149, China; Corresponding author at: Department of Medical Imaging Center Nuclear Medicine, LuHe Hospital, Capital Medical University, No.82, Xinhuanan Road, Tongzhou District, Beijing City 101149, China.Objective: The objective of this study is to investigate the expression of Vascular cell adhesion molecule-1 (VCAM-1) and very late appearing antigen-4 (VLA-4) cytokines in MM (multiple Myeloma). Method: Forty patients with MM are selected as the experimental group and 30 healthy persons as the control group. Flow cytometry is used to detect the expression of VCAM-1 (CD106), VLA-4 (CD49d), CD38 and CD138 antigens in experimental group and control group. ELISA (Enzyme Linked Immunosorbent Assay) is used to detect the concentration of VCAM-1 in serum of experimental group and control group. RT-PCR is used to detect the expression of VCAM-1. Results: The positive rate and antigen expression rate of VACM-1 antigen in the experimental group were significantly higher than those in the control group (P < 0.05). There were statistical differences of VLA-4 and VCAM-1 antigens between the initial diagnosis group and the relapse/refractory group, and between the relapse/refractory group and the platform stage group (P < 0.05). There were significant differences between VLA-4 antigen and VACM-1 antigen, phase I and phase II, and between phase I and phase III (P < 0.05). The concentration of VCAM-1 and the expression of VCAM-1 mRNA in the experimental group were significantly higher than (P < 0.01). In the different stages of ISS (International Staging System) and different disease groups in the experimental group, the concentration of VCAM-1 and the expression level of VCAM-1 mRNA are significantly different among the three groups of stage I, II and III (P < 0.01). There is a significant difference between the initial diagnosis group, the relapse/refractory group and the platform group (P < 0.05). Conclusion: There are abnormal expressions of adhesion molecules VCAM-1 and VLA-4 in multiple myeloma patients, which are related to ISS staging.http://www.sciencedirect.com/science/article/pii/S1319562X20301522Multiple myelomaVCAM-1VLA-4Flow cytometryELISA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pan Hao Chunli Zhang Rongfu Wang Ping Yan Ruchen Peng |
spellingShingle |
Pan Hao Chunli Zhang Rongfu Wang Ping Yan Ruchen Peng Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma Saudi Journal of Biological Sciences Multiple myeloma VCAM-1 VLA-4 Flow cytometry ELISA |
author_facet |
Pan Hao Chunli Zhang Rongfu Wang Ping Yan Ruchen Peng |
author_sort |
Pan Hao |
title |
Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma |
title_short |
Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma |
title_full |
Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma |
title_fullStr |
Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma |
title_full_unstemmed |
Expression and pathogenesis of VCAM-1 and VLA-4 cytokines in multiple myeloma |
title_sort |
expression and pathogenesis of vcam-1 and vla-4 cytokines in multiple myeloma |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2020-06-01 |
description |
Objective: The objective of this study is to investigate the expression of Vascular cell adhesion molecule-1 (VCAM-1) and very late appearing antigen-4 (VLA-4) cytokines in MM (multiple Myeloma). Method: Forty patients with MM are selected as the experimental group and 30 healthy persons as the control group. Flow cytometry is used to detect the expression of VCAM-1 (CD106), VLA-4 (CD49d), CD38 and CD138 antigens in experimental group and control group. ELISA (Enzyme Linked Immunosorbent Assay) is used to detect the concentration of VCAM-1 in serum of experimental group and control group. RT-PCR is used to detect the expression of VCAM-1. Results: The positive rate and antigen expression rate of VACM-1 antigen in the experimental group were significantly higher than those in the control group (P < 0.05). There were statistical differences of VLA-4 and VCAM-1 antigens between the initial diagnosis group and the relapse/refractory group, and between the relapse/refractory group and the platform stage group (P < 0.05). There were significant differences between VLA-4 antigen and VACM-1 antigen, phase I and phase II, and between phase I and phase III (P < 0.05). The concentration of VCAM-1 and the expression of VCAM-1 mRNA in the experimental group were significantly higher than (P < 0.01). In the different stages of ISS (International Staging System) and different disease groups in the experimental group, the concentration of VCAM-1 and the expression level of VCAM-1 mRNA are significantly different among the three groups of stage I, II and III (P < 0.01). There is a significant difference between the initial diagnosis group, the relapse/refractory group and the platform group (P < 0.05). Conclusion: There are abnormal expressions of adhesion molecules VCAM-1 and VLA-4 in multiple myeloma patients, which are related to ISS staging. |
topic |
Multiple myeloma VCAM-1 VLA-4 Flow cytometry ELISA |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X20301522 |
work_keys_str_mv |
AT panhao expressionandpathogenesisofvcam1andvla4cytokinesinmultiplemyeloma AT chunlizhang expressionandpathogenesisofvcam1andvla4cytokinesinmultiplemyeloma AT rongfuwang expressionandpathogenesisofvcam1andvla4cytokinesinmultiplemyeloma AT pingyan expressionandpathogenesisofvcam1andvla4cytokinesinmultiplemyeloma AT ruchenpeng expressionandpathogenesisofvcam1andvla4cytokinesinmultiplemyeloma |
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