Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation

Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation

The possibility that cultured keratinocytes produce endothelins were investigated. The results showed that cultured keratinocytes derived from normal human skin produce endothelin-1. Moreover, keratinocyte endothelin-1 production was completely inhibited by the presence of actinomycin D in the mediu...

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Main Authors: Hajime Inoue, Nagaoki Wakisaka, Nobuhiro Tane, Kazumasa Ando, Emiko Isono, Miwa Yamanaka, Masaki Aihara, Hirotomo Ishida
Format: Article
Language:English
Published: Hindawi Limited 1994-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/S096293519400061X
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spelling doaj-e090fd0bd13646449455bac3494cfa892020-11-24T21:52:08ZengHindawi LimitedMediators of Inflammation0962-93511466-18611994-01-013643343710.1155/S096293519400061XEndothelin-1 Production by Normal Human Cultured Keratinocytes and its RegulationHajime Inoue0Nagaoki Wakisaka1Nobuhiro Tane2Kazumasa Ando3Emiko Isono4Miwa Yamanaka5Masaki Aihara6Hirotomo Ishida7Department of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216, JapanThe possibility that cultured keratinocytes produce endothelins were investigated. The results showed that cultured keratinocytes derived from normal human skin produce endothelin-1. Moreover, keratinocyte endothelin-1 production was completely inhibited by the presence of actinomycin D in the medium. As in the case of endothelial cells, recombinant interleukin-1β was capable of promoting endothelin-1 production in keratinocytes, whereas herapin inhibited it. Thrombin also inhibited endothelin-1 production. These results indicate that the mechanism of endothelin-1 production in keratinocytes is slightly different from the mechanism in vascular endothelial cells.http://dx.doi.org/10.1155/S096293519400061X
collection DOAJ
language English
format Article
sources DOAJ
author Hajime Inoue
Nagaoki Wakisaka
Nobuhiro Tane
Kazumasa Ando
Emiko Isono
Miwa Yamanaka
Masaki Aihara
Hirotomo Ishida
spellingShingle Hajime Inoue
Nagaoki Wakisaka
Nobuhiro Tane
Kazumasa Ando
Emiko Isono
Miwa Yamanaka
Masaki Aihara
Hirotomo Ishida
Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation
Mediators of Inflammation
author_facet Hajime Inoue
Nagaoki Wakisaka
Nobuhiro Tane
Kazumasa Ando
Emiko Isono
Miwa Yamanaka
Masaki Aihara
Hirotomo Ishida
author_sort Hajime Inoue
title Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation
title_short Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation
title_full Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation
title_fullStr Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation
title_full_unstemmed Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation
title_sort endothelin-1 production by normal human cultured keratinocytes and its regulation
publisher Hindawi Limited
series Mediators of Inflammation
issn 0962-9351
1466-1861
publishDate 1994-01-01
description The possibility that cultured keratinocytes produce endothelins were investigated. The results showed that cultured keratinocytes derived from normal human skin produce endothelin-1. Moreover, keratinocyte endothelin-1 production was completely inhibited by the presence of actinomycin D in the medium. As in the case of endothelial cells, recombinant interleukin-1β was capable of promoting endothelin-1 production in keratinocytes, whereas herapin inhibited it. Thrombin also inhibited endothelin-1 production. These results indicate that the mechanism of endothelin-1 production in keratinocytes is slightly different from the mechanism in vascular endothelial cells.
url http://dx.doi.org/10.1155/S096293519400061X
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AT nagaokiwakisaka endothelin1productionbynormalhumanculturedkeratinocytesanditsregulation
AT nobuhirotane endothelin1productionbynormalhumanculturedkeratinocytesanditsregulation
AT kazumasaando endothelin1productionbynormalhumanculturedkeratinocytesanditsregulation
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AT miwayamanaka endothelin1productionbynormalhumanculturedkeratinocytesanditsregulation
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