Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
<p>Abstract</p> <p>Background</p> <p>Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, av...
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doaj-e097b368f5d64708bb49924daf672d852021-04-02T18:05:10ZengBMCBMC Medical Genomics1755-87942010-02-0131510.1186/1755-8794-3-5Insight in modulation of inflammation in response to diclofenac intervention: a human intervention studyNewman John WPedersen Theresa LCnubben Nicole HPVerheij Elwinvan Vliet TrinetteRubingh CarinaWopereis Suzanvan Erk Marjan JSmilde Age KGreef JanHendriks Henk FJvan Ommen Ben<p>Abstract</p> <p>Background</p> <p>Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, available markers of inflammatory status inadequately describe the complexity of metabolic responses to mild anti-inflammatory therapy.</p> <p>Methods</p> <p>To address this limitation, we used an integrative omics approach to characterize modulation of inflammation in overweight men during an intervention with the non-steroidal anti-inflammatory drug diclofenac. Measured parameters included 80 plasma proteins, >300 plasma metabolites (lipids, free fatty acids, oxylipids and polar compounds) and an array of peripheral blood mononuclear cells (PBMC) gene expression products. These measures were submitted to multivariate and correlation analysis and were used for construction of biological response networks.</p> <p>Results</p> <p>A panel of genes, proteins and metabolites, including PGE<sub>2 </sub>and TNF-alpha, were identified that describe a diclofenac-response network (68 genes in PBMC, 1 plasma protein and 4 plasma metabolites). Novel candidate markers of inflammatory modulation included PBMC expression of annexin A1 and caspase 8, and the arachidonic acid metabolite 5,6-DHET.</p> <p>Conclusion</p> <p>In this study the integrated analysis of a wide range of parameters allowed the development of a network of markers responding to inflammatory modulation, thereby providing insight into the complex process of inflammation and ways to assess changes in inflammatory status associated with obesity.</p> <p>Trial registration</p> <p>The study is registered as NCT00221052 in clinicaltrials.gov database.</p> http://www.biomedcentral.com/1755-8794/3/5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Newman John W Pedersen Theresa L Cnubben Nicole HP Verheij Elwin van Vliet Trinette Rubingh Carina Wopereis Suzan van Erk Marjan J Smilde Age K Greef Jan Hendriks Henk FJ van Ommen Ben |
spellingShingle |
Newman John W Pedersen Theresa L Cnubben Nicole HP Verheij Elwin van Vliet Trinette Rubingh Carina Wopereis Suzan van Erk Marjan J Smilde Age K Greef Jan Hendriks Henk FJ van Ommen Ben Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study BMC Medical Genomics |
author_facet |
Newman John W Pedersen Theresa L Cnubben Nicole HP Verheij Elwin van Vliet Trinette Rubingh Carina Wopereis Suzan van Erk Marjan J Smilde Age K Greef Jan Hendriks Henk FJ van Ommen Ben |
author_sort |
Newman John W |
title |
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study |
title_short |
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study |
title_full |
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study |
title_fullStr |
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study |
title_full_unstemmed |
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study |
title_sort |
insight in modulation of inflammation in response to diclofenac intervention: a human intervention study |
publisher |
BMC |
series |
BMC Medical Genomics |
issn |
1755-8794 |
publishDate |
2010-02-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, available markers of inflammatory status inadequately describe the complexity of metabolic responses to mild anti-inflammatory therapy.</p> <p>Methods</p> <p>To address this limitation, we used an integrative omics approach to characterize modulation of inflammation in overweight men during an intervention with the non-steroidal anti-inflammatory drug diclofenac. Measured parameters included 80 plasma proteins, >300 plasma metabolites (lipids, free fatty acids, oxylipids and polar compounds) and an array of peripheral blood mononuclear cells (PBMC) gene expression products. These measures were submitted to multivariate and correlation analysis and were used for construction of biological response networks.</p> <p>Results</p> <p>A panel of genes, proteins and metabolites, including PGE<sub>2 </sub>and TNF-alpha, were identified that describe a diclofenac-response network (68 genes in PBMC, 1 plasma protein and 4 plasma metabolites). Novel candidate markers of inflammatory modulation included PBMC expression of annexin A1 and caspase 8, and the arachidonic acid metabolite 5,6-DHET.</p> <p>Conclusion</p> <p>In this study the integrated analysis of a wide range of parameters allowed the development of a network of markers responding to inflammatory modulation, thereby providing insight into the complex process of inflammation and ways to assess changes in inflammatory status associated with obesity.</p> <p>Trial registration</p> <p>The study is registered as NCT00221052 in clinicaltrials.gov database.</p> |
url |
http://www.biomedcentral.com/1755-8794/3/5 |
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