Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches
In the last decade the role of non coding (nc)RNAs in neurogenesis and in the onset of neurological diseases has been assessed by a multitude of studies. In this scenario, approximately 30 small RNA polymerase (pol) III–dependent ncRNAs were recently identified by computational tools and proposed as...
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doaj-e09858f5388a448b932bd61e96dce46b2020-11-24T21:13:46ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022013-11-01710.3389/fncel.2013.0020360451Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approachesPaola eGavazzo0Massimo eVassalli1Delfina eCosta2Aldo ePagano3Aldo ePagano4National Research CouncilNational Research CouncilUniversity of GenoaUniversity of GenoaIRCCS-AOU San Martino-ISTIn the last decade the role of non coding (nc)RNAs in neurogenesis and in the onset of neurological diseases has been assessed by a multitude of studies. In this scenario, approximately 30 small RNA polymerase (pol) III–dependent ncRNAs were recently identified by computational tools and proposed as regulatory elements. The function of several of these transcripts was elucidated in vitro and in vivo confirming their involvement in cancer and in metabolic and neurodegenerative disorders. Emerging biophysical technologies together with the introduction of a physical perspective have been advantageous in regulatory RNA investigation providing original results on: a) the differentiation of neuroblastoma (NB) cells towards a neuron-like phenotype triggered by NDM29 ncRNA b) the modulation of A-type K+ current in neurons induced by the small ncRNA 38A and c) the synthesis driven by 17A ncRNA of a GABAB2 receptor isoform unable to trigger intracellular signalling. Moreover, the application of Single Cell Force Spectroscopy to these studies suggests a correlation between the malignancy stage of NB and the micro-adhesive properties of the cells, allowing to investigate the molecular basis of such a correlation.http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00203/fullNeuroblastomaRNA Polymerase IIInon-coding RNApatch clampSingle Cell Force Spectroscopy (SCFS) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paola eGavazzo Massimo eVassalli Delfina eCosta Aldo ePagano Aldo ePagano |
spellingShingle |
Paola eGavazzo Massimo eVassalli Delfina eCosta Aldo ePagano Aldo ePagano Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches Frontiers in Cellular Neuroscience Neuroblastoma RNA Polymerase III non-coding RNA patch clamp Single Cell Force Spectroscopy (SCFS) |
author_facet |
Paola eGavazzo Massimo eVassalli Delfina eCosta Aldo ePagano Aldo ePagano |
author_sort |
Paola eGavazzo |
title |
Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches |
title_short |
Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches |
title_full |
Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches |
title_fullStr |
Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches |
title_full_unstemmed |
Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches |
title_sort |
novel ncrnas transcribed by pol iii and elucidation of their functional relevance by biophysical approaches |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2013-11-01 |
description |
In the last decade the role of non coding (nc)RNAs in neurogenesis and in the onset of neurological diseases has been assessed by a multitude of studies. In this scenario, approximately 30 small RNA polymerase (pol) III–dependent ncRNAs were recently identified by computational tools and proposed as regulatory elements. The function of several of these transcripts was elucidated in vitro and in vivo confirming their involvement in cancer and in metabolic and neurodegenerative disorders. Emerging biophysical technologies together with the introduction of a physical perspective have been advantageous in regulatory RNA investigation providing original results on: a) the differentiation of neuroblastoma (NB) cells towards a neuron-like phenotype triggered by NDM29 ncRNA b) the modulation of A-type K+ current in neurons induced by the small ncRNA 38A and c) the synthesis driven by 17A ncRNA of a GABAB2 receptor isoform unable to trigger intracellular signalling. Moreover, the application of Single Cell Force Spectroscopy to these studies suggests a correlation between the malignancy stage of NB and the micro-adhesive properties of the cells, allowing to investigate the molecular basis of such a correlation. |
topic |
Neuroblastoma RNA Polymerase III non-coding RNA patch clamp Single Cell Force Spectroscopy (SCFS) |
url |
http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00203/full |
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