Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
Background: The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal responses in the upper airways, the site of initial SARS-CoV-2 replication. Methods: The IgG and IgA antibody responses were analysed in plasma and na...
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doaj-e0b5f124ea41424f8126e93b7c65a09e2021-10-07T04:27:15ZengElsevierJournal of Clinical Virology Plus2667-03802021-12-0114100041Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patientsBernadette Crescenzo-Chaigne0Sylvie Behillil1Vincent Enouf2Nicolas Escriou3Stephane Petres4Marie Noelle Ungeheuer5Jade Ghosn6Sarah Tubiana7Lila Bouadma8Sylvie van der Werf9Caroline Demeret10Molecular Genetics of RNA Viruses Unit, Institut Pasteur, UMR3569, CNRS, Université de Paris, 28 rue du Docteur Roux, 75015 Paris, FranceNational Reference Center for Respiratory Viruses, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceNational Reference Center for Respiratory Viruses, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceInnovation lab: vaccines, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceProduction and Purification of Recombinant Proteins Technological Platform, Center for technological resources and research, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceICAReB platform, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, FranceInfectious and Tropical Diseases Department, Assistance Publique-Hôpitaux de Paris, Bichat-Claude Bernard University Hospital, Université de Paris, INSERM, IAME UMR 1137, Paris, FranceCenter for Clinical Investigation, Assistance Publique-Hôpitaux de Paris, Bichat-Claude Bernard University Hospital, Université de Paris, INSERM, IAME UMR 1137, Paris, Paris, FranceMedical and Infectious Diseases Intensive Care Unit, Assistance Publique-Hôpitaux de Paris, Bichat-Claude Bernard University Hospital, Université de Paris, INSERM, IAME UMR 1137, Paris, FranceMolecular Genetics of RNA Viruses Unit, Institut Pasteur, UMR3569, CNRS, Université de Paris, 28 rue du Docteur Roux, 75015 Paris, France; National Reference Center for Respiratory Viruses, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, France; Corresponding authors.Molecular Genetics of RNA Viruses Unit, Institut Pasteur, UMR3569, CNRS, Université de Paris, 28 rue du Docteur Roux, 75015 Paris, France; Corresponding authors.Background: The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal responses in the upper airways, the site of initial SARS-CoV-2 replication. Methods: The IgG and IgA antibody responses were analysed in plasma and nasopharyngeal swabs from the first four confirmed COVID-19 patients in France. Two were pauci-symptomatic while two developed severe disease. We characterized their antibody profiles by using an in-house ELISA to detect antibodies directed against SARS-CoV-2 Nucleoprotein and Spike. Results: Anti-N IgG and IgA antibodies were detected in the NPS of severe patients only. The levels of antibodies in the plasma markedly differed amongst the patients. The most distinctive features are a strong anti-N IgG response in the severe patient who recovered, and a high anti-N IgA response specifically detected in the fatal case of COVID-19. Conclusions: Anti-N IgG and IgA antibodies are detected in NPS only for severe patients, with levels related to serological antibodies. The severe patients showed different antibody profiles in the plasma, notably regarding the IgA and IgG response to the N antigen, that may reflect different disease outcome. By contrast, pauci-symptomatic patients did not exhibit any mucosal antibodies in NSP, which is associated with a low or absent serological response against both N and S.http://www.sciencedirect.com/science/article/pii/S2667038021000338SARS CoVMucosal ImmunitySerology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bernadette Crescenzo-Chaigne Sylvie Behillil Vincent Enouf Nicolas Escriou Stephane Petres Marie Noelle Ungeheuer Jade Ghosn Sarah Tubiana Lila Bouadma Sylvie van der Werf Caroline Demeret |
spellingShingle |
Bernadette Crescenzo-Chaigne Sylvie Behillil Vincent Enouf Nicolas Escriou Stephane Petres Marie Noelle Ungeheuer Jade Ghosn Sarah Tubiana Lila Bouadma Sylvie van der Werf Caroline Demeret Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients Journal of Clinical Virology Plus SARS CoV Mucosal Immunity Serology |
author_facet |
Bernadette Crescenzo-Chaigne Sylvie Behillil Vincent Enouf Nicolas Escriou Stephane Petres Marie Noelle Ungeheuer Jade Ghosn Sarah Tubiana Lila Bouadma Sylvie van der Werf Caroline Demeret |
author_sort |
Bernadette Crescenzo-Chaigne |
title |
Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients |
title_short |
Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients |
title_full |
Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients |
title_fullStr |
Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients |
title_full_unstemmed |
Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients |
title_sort |
nasopharyngeal and serological anti sars-cov-2 igg/iga responses in covid-19 patients |
publisher |
Elsevier |
series |
Journal of Clinical Virology Plus |
issn |
2667-0380 |
publishDate |
2021-12-01 |
description |
Background: The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal responses in the upper airways, the site of initial SARS-CoV-2 replication. Methods: The IgG and IgA antibody responses were analysed in plasma and nasopharyngeal swabs from the first four confirmed COVID-19 patients in France. Two were pauci-symptomatic while two developed severe disease. We characterized their antibody profiles by using an in-house ELISA to detect antibodies directed against SARS-CoV-2 Nucleoprotein and Spike. Results: Anti-N IgG and IgA antibodies were detected in the NPS of severe patients only. The levels of antibodies in the plasma markedly differed amongst the patients. The most distinctive features are a strong anti-N IgG response in the severe patient who recovered, and a high anti-N IgA response specifically detected in the fatal case of COVID-19. Conclusions: Anti-N IgG and IgA antibodies are detected in NPS only for severe patients, with levels related to serological antibodies. The severe patients showed different antibody profiles in the plasma, notably regarding the IgA and IgG response to the N antigen, that may reflect different disease outcome. By contrast, pauci-symptomatic patients did not exhibit any mucosal antibodies in NSP, which is associated with a low or absent serological response against both N and S. |
topic |
SARS CoV Mucosal Immunity Serology |
url |
http://www.sciencedirect.com/science/article/pii/S2667038021000338 |
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