Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients

Background: The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal responses in the upper airways, the site of initial SARS-CoV-2 replication. Methods: The IgG and IgA antibody responses were analysed in plasma and na...

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Main Authors: Bernadette Crescenzo-Chaigne, Sylvie Behillil, Vincent Enouf, Nicolas Escriou, Stephane Petres, Marie Noelle Ungeheuer, Jade Ghosn, Sarah Tubiana, Lila Bouadma, Sylvie van der Werf, Caroline Demeret
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Journal of Clinical Virology Plus
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667038021000338
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spelling doaj-e0b5f124ea41424f8126e93b7c65a09e2021-10-07T04:27:15ZengElsevierJournal of Clinical Virology Plus2667-03802021-12-0114100041Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patientsBernadette Crescenzo-Chaigne0Sylvie Behillil1Vincent Enouf2Nicolas Escriou3Stephane Petres4Marie Noelle Ungeheuer5Jade Ghosn6Sarah Tubiana7Lila Bouadma8Sylvie van der Werf9Caroline Demeret10Molecular Genetics of RNA Viruses Unit, Institut Pasteur, UMR3569, CNRS, Université de Paris, 28 rue du Docteur Roux, 75015 Paris, FranceNational Reference Center for Respiratory Viruses, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceNational Reference Center for Respiratory Viruses, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceInnovation lab: vaccines, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceProduction and Purification of Recombinant Proteins Technological Platform, Center for technological resources and research, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, FranceICAReB platform, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, FranceInfectious and Tropical Diseases Department, Assistance Publique-Hôpitaux de Paris, Bichat-Claude Bernard University Hospital, Université de Paris, INSERM, IAME UMR 1137, Paris, FranceCenter for Clinical Investigation, Assistance Publique-Hôpitaux de Paris, Bichat-Claude Bernard University Hospital, Université de Paris, INSERM, IAME UMR 1137, Paris, Paris, FranceMedical and Infectious Diseases Intensive Care Unit, Assistance Publique-Hôpitaux de Paris, Bichat-Claude Bernard University Hospital, Université de Paris, INSERM, IAME UMR 1137, Paris, FranceMolecular Genetics of RNA Viruses Unit, Institut Pasteur, UMR3569, CNRS, Université de Paris, 28 rue du Docteur Roux, 75015 Paris, France; National Reference Center for Respiratory Viruses, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, France; Corresponding authors.Molecular Genetics of RNA Viruses Unit, Institut Pasteur, UMR3569, CNRS, Université de Paris, 28 rue du Docteur Roux, 75015 Paris, France; Corresponding authors.Background: The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal responses in the upper airways, the site of initial SARS-CoV-2 replication. Methods: The IgG and IgA antibody responses were analysed in plasma and nasopharyngeal swabs from the first four confirmed COVID-19 patients in France. Two were pauci-symptomatic while two developed severe disease. We characterized their antibody profiles by using an in-house ELISA to detect antibodies directed against SARS-CoV-2 Nucleoprotein and Spike. Results: Anti-N IgG and IgA antibodies were detected in the NPS of severe patients only. The levels of antibodies in the plasma markedly differed amongst the patients. The most distinctive features are a strong anti-N IgG response in the severe patient who recovered, and a high anti-N IgA response specifically detected in the fatal case of COVID-19. Conclusions: Anti-N IgG and IgA antibodies are detected in NPS only for severe patients, with levels related to serological antibodies. The severe patients showed different antibody profiles in the plasma, notably regarding the IgA and IgG response to the N antigen, that may reflect different disease outcome. By contrast, pauci-symptomatic patients did not exhibit any mucosal antibodies in NSP, which is associated with a low or absent serological response against both N and S.http://www.sciencedirect.com/science/article/pii/S2667038021000338SARS CoVMucosal ImmunitySerology
collection DOAJ
language English
format Article
sources DOAJ
author Bernadette Crescenzo-Chaigne
Sylvie Behillil
Vincent Enouf
Nicolas Escriou
Stephane Petres
Marie Noelle Ungeheuer
Jade Ghosn
Sarah Tubiana
Lila Bouadma
Sylvie van der Werf
Caroline Demeret
spellingShingle Bernadette Crescenzo-Chaigne
Sylvie Behillil
Vincent Enouf
Nicolas Escriou
Stephane Petres
Marie Noelle Ungeheuer
Jade Ghosn
Sarah Tubiana
Lila Bouadma
Sylvie van der Werf
Caroline Demeret
Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
Journal of Clinical Virology Plus
SARS CoV
Mucosal Immunity
Serology
author_facet Bernadette Crescenzo-Chaigne
Sylvie Behillil
Vincent Enouf
Nicolas Escriou
Stephane Petres
Marie Noelle Ungeheuer
Jade Ghosn
Sarah Tubiana
Lila Bouadma
Sylvie van der Werf
Caroline Demeret
author_sort Bernadette Crescenzo-Chaigne
title Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
title_short Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
title_full Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
title_fullStr Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
title_full_unstemmed Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients
title_sort nasopharyngeal and serological anti sars-cov-2 igg/iga responses in covid-19 patients
publisher Elsevier
series Journal of Clinical Virology Plus
issn 2667-0380
publishDate 2021-12-01
description Background: The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, less is known about the mucosal responses in the upper airways, the site of initial SARS-CoV-2 replication. Methods: The IgG and IgA antibody responses were analysed in plasma and nasopharyngeal swabs from the first four confirmed COVID-19 patients in France. Two were pauci-symptomatic while two developed severe disease. We characterized their antibody profiles by using an in-house ELISA to detect antibodies directed against SARS-CoV-2 Nucleoprotein and Spike. Results: Anti-N IgG and IgA antibodies were detected in the NPS of severe patients only. The levels of antibodies in the plasma markedly differed amongst the patients. The most distinctive features are a strong anti-N IgG response in the severe patient who recovered, and a high anti-N IgA response specifically detected in the fatal case of COVID-19. Conclusions: Anti-N IgG and IgA antibodies are detected in NPS only for severe patients, with levels related to serological antibodies. The severe patients showed different antibody profiles in the plasma, notably regarding the IgA and IgG response to the N antigen, that may reflect different disease outcome. By contrast, pauci-symptomatic patients did not exhibit any mucosal antibodies in NSP, which is associated with a low or absent serological response against both N and S.
topic SARS CoV
Mucosal Immunity
Serology
url http://www.sciencedirect.com/science/article/pii/S2667038021000338
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