Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma
Background Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to...
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doaj-e0d0253a103d42fcaf17bf7a55c14e5d2021-06-12T15:05:12ZengPeerJ Inc.PeerJ2167-83592021-06-019e1154810.7717/peerj.11548Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinomaYin-Ling Wong0Anand Ramanathan1Kar Mun Yuen2Wan Mahadzir Wan Mustafa3Mannil Thomas Abraham4Keng Kiong Tay5Zainal Ariff Abdul Rahman6Yeng Chen7Department of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, MalaysiaDepartment of Oral & Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, MalaysiaOral Health Program, Ministry of Health, Federal Government Administrative Centre, Putrajaya, MalaysiaFaculty of Dentistry, MAHSA University, Jenjarum, Selangor, MalaysiaOral Health Program, Ministry of Health, Federal Government Administrative Centre, Putrajaya, MalaysiaOral Health Program, Ministry of Health, Federal Government Administrative Centre, Putrajaya, MalaysiaDepartment of Oral & Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, MalaysiaDepartment of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, MalaysiaBackground Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to identify the differentially expressed serum proteins as potential biomarkers for oral squamous cell carcinoma (OSCC). Methods Comparative proteomics profiling of serum samples from OSCC patients, oral potentially malignant disorder (OPMD) patients, and healthy individuals were performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) (n = 60) and bioinformatics analysis. The enzyme-linked immunosorbent assay (ELISA) (n = 120) and immunohistochemistry (IHC) (n = 70) were used to confirm our findings. Results The 2-DE analysis revealed that 20 differentially expressed proteins were detected in OPMD and OSCC (p < 0.05). Bioinformatics analysis indicated that the activation of classical complement, liver X receptor/retinoid X receptor (LXR/RXR) activation, and acute phase response signaling pathway are associated with the development and progression of OSCC. Most of the detected proteins are acute-phase proteins and were related to inflammation and immune responses, including apolipoprotein A-I (APOA1), complement C3 (C3), clusterin (CLU), and haptoglobin (HP). The expression levels of CLU and HP in ELISA are consistent with the findings from the 2-DE analysis, except for the mean serum level of HP in OPMD, whereby it was slightly higher than that in control. IHC results demonstrated that CLU and HP are significantly decreased in OSCC tissues. Conclusion Decreased expression of CLU and HP could serve as complementary biomarkers of OSCC. These proteins may assist in predicting the outcomes of OSCC patients. However, a larger cohort is needed for further investigation.https://peerj.com/articles/11548.pdfBiomarkerProteomicsOral potentially malignant disorderOral squamous cell carcinoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin-Ling Wong Anand Ramanathan Kar Mun Yuen Wan Mahadzir Wan Mustafa Mannil Thomas Abraham Keng Kiong Tay Zainal Ariff Abdul Rahman Yeng Chen |
spellingShingle |
Yin-Ling Wong Anand Ramanathan Kar Mun Yuen Wan Mahadzir Wan Mustafa Mannil Thomas Abraham Keng Kiong Tay Zainal Ariff Abdul Rahman Yeng Chen Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma PeerJ Biomarker Proteomics Oral potentially malignant disorder Oral squamous cell carcinoma |
author_facet |
Yin-Ling Wong Anand Ramanathan Kar Mun Yuen Wan Mahadzir Wan Mustafa Mannil Thomas Abraham Keng Kiong Tay Zainal Ariff Abdul Rahman Yeng Chen |
author_sort |
Yin-Ling Wong |
title |
Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma |
title_short |
Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma |
title_full |
Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma |
title_fullStr |
Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma |
title_full_unstemmed |
Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma |
title_sort |
comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2021-06-01 |
description |
Background Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to identify the differentially expressed serum proteins as potential biomarkers for oral squamous cell carcinoma (OSCC). Methods Comparative proteomics profiling of serum samples from OSCC patients, oral potentially malignant disorder (OPMD) patients, and healthy individuals were performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) (n = 60) and bioinformatics analysis. The enzyme-linked immunosorbent assay (ELISA) (n = 120) and immunohistochemistry (IHC) (n = 70) were used to confirm our findings. Results The 2-DE analysis revealed that 20 differentially expressed proteins were detected in OPMD and OSCC (p < 0.05). Bioinformatics analysis indicated that the activation of classical complement, liver X receptor/retinoid X receptor (LXR/RXR) activation, and acute phase response signaling pathway are associated with the development and progression of OSCC. Most of the detected proteins are acute-phase proteins and were related to inflammation and immune responses, including apolipoprotein A-I (APOA1), complement C3 (C3), clusterin (CLU), and haptoglobin (HP). The expression levels of CLU and HP in ELISA are consistent with the findings from the 2-DE analysis, except for the mean serum level of HP in OPMD, whereby it was slightly higher than that in control. IHC results demonstrated that CLU and HP are significantly decreased in OSCC tissues. Conclusion Decreased expression of CLU and HP could serve as complementary biomarkers of OSCC. These proteins may assist in predicting the outcomes of OSCC patients. However, a larger cohort is needed for further investigation. |
topic |
Biomarker Proteomics Oral potentially malignant disorder Oral squamous cell carcinoma |
url |
https://peerj.com/articles/11548.pdf |
work_keys_str_mv |
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