Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation
Anorexia nervosa (AN) is a life-threatening psychiatric disorder with not well-described pathogenesis. Besides the genetic and sociological factors, autoimmunity is also considered to take part in AN pathogenesis. We evaluated general serological factors showing the physiological state of 59 patient...
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doaj-e0d0a655498146ca8eede887bd2bf11f2021-08-02T04:54:22ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-08-01810.3389/fnut.2021.680870680870Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During RealimentationRadka Roubalova0Petra Prochazkova1Jiri Dvorak2Martin Hill3Hana Papezova4Jakub Kreisinger5Josef Bulant6Josef Bulant7Alena Lambertova8Petra Holanova9Martin Bilej10Helena Tlaskalova-Hogenova11Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaDepartment of Steroids and Proteohormones, Institute of Endocrinology, Prague, CzechiaDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, CzechiaDepartment of Zoology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, CzechiaDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, CzechiaDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, CzechiaDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaAnorexia nervosa (AN) is a life-threatening psychiatric disorder with not well-described pathogenesis. Besides the genetic and sociological factors, autoimmunity is also considered to take part in AN pathogenesis. We evaluated general serological factors showing the physiological state of 59 patients with AN at hospital admission and their discharge. We detected the altered levels of some general biochemical and immunological parameters. We also detected decreased levels of appetite-regulating alpha-melanocyte stimulating hormone (α-MSH) in patients at hospital admission. Moreover, elevated anti-α-MSH IgM levels and decreased anti-α-MSH IgA levels were observed in patients with AN. Therefore, we analyzed the gut microbiota composition with special focus on α-MSH antigen-mimetic containing microbes from the Enterobacteriaceae family. We correlated gut bacterial composition with anti-α-MSH Ig levels and detected decreasing IgG levels with increasing alpha diversity. The upregulation of pro-inflammatory cytokines IL-6, IL-17, and TNF-α were detected in patients with AN both prior and after hospitalization. We also evaluated the treatment outcome and improvement was observed in the majority of patients with AN. We provide new data about various serum biochemical parameters and their changes during the patients' hospitalization, with emphasis on the immune system, and its possible participation in AN pathogenesis.https://www.frontiersin.org/articles/10.3389/fnut.2021.680870/fullanorexia nervosarealimentationalpha-melanocyte stimulating hormoneimmune systemautoantibodyEnterobacteriaceae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Radka Roubalova Petra Prochazkova Jiri Dvorak Martin Hill Hana Papezova Jakub Kreisinger Josef Bulant Josef Bulant Alena Lambertova Petra Holanova Martin Bilej Helena Tlaskalova-Hogenova |
spellingShingle |
Radka Roubalova Petra Prochazkova Jiri Dvorak Martin Hill Hana Papezova Jakub Kreisinger Josef Bulant Josef Bulant Alena Lambertova Petra Holanova Martin Bilej Helena Tlaskalova-Hogenova Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation Frontiers in Nutrition anorexia nervosa realimentation alpha-melanocyte stimulating hormone immune system autoantibody Enterobacteriaceae |
author_facet |
Radka Roubalova Petra Prochazkova Jiri Dvorak Martin Hill Hana Papezova Jakub Kreisinger Josef Bulant Josef Bulant Alena Lambertova Petra Holanova Martin Bilej Helena Tlaskalova-Hogenova |
author_sort |
Radka Roubalova |
title |
Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation |
title_short |
Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation |
title_full |
Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation |
title_fullStr |
Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation |
title_full_unstemmed |
Altered Serum Immunological and Biochemical Parameters and Microbiota Composition in Patients With AN During Realimentation |
title_sort |
altered serum immunological and biochemical parameters and microbiota composition in patients with an during realimentation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Nutrition |
issn |
2296-861X |
publishDate |
2021-08-01 |
description |
Anorexia nervosa (AN) is a life-threatening psychiatric disorder with not well-described pathogenesis. Besides the genetic and sociological factors, autoimmunity is also considered to take part in AN pathogenesis. We evaluated general serological factors showing the physiological state of 59 patients with AN at hospital admission and their discharge. We detected the altered levels of some general biochemical and immunological parameters. We also detected decreased levels of appetite-regulating alpha-melanocyte stimulating hormone (α-MSH) in patients at hospital admission. Moreover, elevated anti-α-MSH IgM levels and decreased anti-α-MSH IgA levels were observed in patients with AN. Therefore, we analyzed the gut microbiota composition with special focus on α-MSH antigen-mimetic containing microbes from the Enterobacteriaceae family. We correlated gut bacterial composition with anti-α-MSH Ig levels and detected decreasing IgG levels with increasing alpha diversity. The upregulation of pro-inflammatory cytokines IL-6, IL-17, and TNF-α were detected in patients with AN both prior and after hospitalization. We also evaluated the treatment outcome and improvement was observed in the majority of patients with AN. We provide new data about various serum biochemical parameters and their changes during the patients' hospitalization, with emphasis on the immune system, and its possible participation in AN pathogenesis. |
topic |
anorexia nervosa realimentation alpha-melanocyte stimulating hormone immune system autoantibody Enterobacteriaceae |
url |
https://www.frontiersin.org/articles/10.3389/fnut.2021.680870/full |
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