Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes
In previous work we have isolated and identified a new strain called Enterococcus faecium FL31. The active compound secreted by this strain, “BacFL31”, has been purified and characterized. In the present study, safety aspect, assessed by microbiological and molecular tests, demonstrated that Enteroc...
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doaj-e0d42853af0c4a409ab263ee8ea7e9ea2020-11-24T20:42:50ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/53084645308464Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenesAhlem Chakchouk-Mtibaa0Imen Sellem1Yosra Kamoun2Slim Smaoui3Ines Karray-Rebai4Lotfi Mellouli5Laboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, TunisiaLaboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, TunisiaLaboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, TunisiaLaboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, TunisiaLaboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, TunisiaLaboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, TunisiaIn previous work we have isolated and identified a new strain called Enterococcus faecium FL31. The active compound secreted by this strain, “BacFL31”, has been purified and characterized. In the present study, safety aspect, assessed by microbiological and molecular tests, demonstrated that Enterococcus faecium FL31 was susceptible to relevant antibiotics, free of hemolytic, gelatinase, DNase, and lipase activities. In addition, it did not harbor virulence and antibiotic resistance genes. Combined SYTOX Green dye and UV-absorbing experiments, along with released extracellular potassium and transmembrane electrical potential measurements, showed that pure BacFL31 at a concentration of 1×MIC (50 μg/mL) could damage cytoplasmic membrane of the pathogen Listeria monocytogenes ATCC19117. The same concentration causes the leakage of its intracellular constituents and leads to the destruction of this pathogenic microorganism. In summary, this work reflected characteristics of Enterococcus faecium FL31 strain and its bacteriocin in terms of functional and safety perspectives.http://dx.doi.org/10.1155/2018/5308464 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahlem Chakchouk-Mtibaa Imen Sellem Yosra Kamoun Slim Smaoui Ines Karray-Rebai Lotfi Mellouli |
spellingShingle |
Ahlem Chakchouk-Mtibaa Imen Sellem Yosra Kamoun Slim Smaoui Ines Karray-Rebai Lotfi Mellouli Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes BioMed Research International |
author_facet |
Ahlem Chakchouk-Mtibaa Imen Sellem Yosra Kamoun Slim Smaoui Ines Karray-Rebai Lotfi Mellouli |
author_sort |
Ahlem Chakchouk-Mtibaa |
title |
Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes |
title_short |
Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes |
title_full |
Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes |
title_fullStr |
Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes |
title_full_unstemmed |
Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes |
title_sort |
safety aspect of enterococcus faecium fl31 strain and antibacterial mechanism of its hydroxylated bacteriocin bacfl31 against listeria monocytogenes |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
In previous work we have isolated and identified a new strain called Enterococcus faecium FL31. The active compound secreted by this strain, “BacFL31”, has been purified and characterized. In the present study, safety aspect, assessed by microbiological and molecular tests, demonstrated that Enterococcus faecium FL31 was susceptible to relevant antibiotics, free of hemolytic, gelatinase, DNase, and lipase activities. In addition, it did not harbor virulence and antibiotic resistance genes. Combined SYTOX Green dye and UV-absorbing experiments, along with released extracellular potassium and transmembrane electrical potential measurements, showed that pure BacFL31 at a concentration of 1×MIC (50 μg/mL) could damage cytoplasmic membrane of the pathogen Listeria monocytogenes ATCC19117. The same concentration causes the leakage of its intracellular constituents and leads to the destruction of this pathogenic microorganism. In summary, this work reflected characteristics of Enterococcus faecium FL31 strain and its bacteriocin in terms of functional and safety perspectives. |
url |
http://dx.doi.org/10.1155/2018/5308464 |
work_keys_str_mv |
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