Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation
ABSTRACT Lignocellulose-derived inhibitors have negative effects on the ethanol fermentation capacity of Saccharomyces cerevisiae. In this study, the effects of eight typical inhibitors, including weak acids, furans, and phenols, on glucose and xylose co-fermentation of the recombinant xylose-fermen...
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doaj-e0db5cda370b47319f966dc63cadd44f2020-11-24T23:18:00ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-440548479180010.1016/j.bjm.2016.11.011S1517-83822017000400791Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentationYun-Cheng LiZi-Xi GouYing ZhangZi-Yuan XiaYue-Qin TangKenji KidaABSTRACT Lignocellulose-derived inhibitors have negative effects on the ethanol fermentation capacity of Saccharomyces cerevisiae. In this study, the effects of eight typical inhibitors, including weak acids, furans, and phenols, on glucose and xylose co-fermentation of the recombinant xylose-fermenting flocculating industrial S. cerevisiae strain NAPX37 were evaluated by batch fermentation. Inhibition on glucose fermentation, not that on xylose fermentation, correlated with delayed cell growth. The weak acids and the phenols showed additive effects. The effect of inhibitors on glucose fermentation was as follows (from strongest to weakest): vanillin > phenol > syringaldehyde > 5-HMF > furfural > levulinic acid > acetic acid > formic acid. The effect of inhibitors on xylose fermentation was as follows (from strongest to weakest): phenol > vanillin > syringaldehyde > furfural > 5-HMF > formic acid > levulinic acid > acetic acid. The NAPX37 strain showed substantial tolerance to typical inhibitors and showed good fermentation characteristics, when a medium with inhibitor cocktail or rape straw hydrolysate was used. This research provides important clues for inhibitors tolerance of recombinant industrial xylose-fermenting S. cerevisiae.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400791&lng=en&tlng=enIndustrial Saccharomyces cerevisiaeBioethanolGlucose and xylose co-fermentationInhibitor tolerance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun-Cheng Li Zi-Xi Gou Ying Zhang Zi-Yuan Xia Yue-Qin Tang Kenji Kida |
spellingShingle |
Yun-Cheng Li Zi-Xi Gou Ying Zhang Zi-Yuan Xia Yue-Qin Tang Kenji Kida Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation Brazilian Journal of Microbiology Industrial Saccharomyces cerevisiae Bioethanol Glucose and xylose co-fermentation Inhibitor tolerance |
author_facet |
Yun-Cheng Li Zi-Xi Gou Ying Zhang Zi-Yuan Xia Yue-Qin Tang Kenji Kida |
author_sort |
Yun-Cheng Li |
title |
Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation |
title_short |
Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation |
title_full |
Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation |
title_fullStr |
Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation |
title_full_unstemmed |
Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation |
title_sort |
inhibitor tolerance of a recombinant flocculating industrial saccharomyces cerevisiae strain during glucose and xylose co-fermentation |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
description |
ABSTRACT Lignocellulose-derived inhibitors have negative effects on the ethanol fermentation capacity of Saccharomyces cerevisiae. In this study, the effects of eight typical inhibitors, including weak acids, furans, and phenols, on glucose and xylose co-fermentation of the recombinant xylose-fermenting flocculating industrial S. cerevisiae strain NAPX37 were evaluated by batch fermentation. Inhibition on glucose fermentation, not that on xylose fermentation, correlated with delayed cell growth. The weak acids and the phenols showed additive effects. The effect of inhibitors on glucose fermentation was as follows (from strongest to weakest): vanillin > phenol > syringaldehyde > 5-HMF > furfural > levulinic acid > acetic acid > formic acid. The effect of inhibitors on xylose fermentation was as follows (from strongest to weakest): phenol > vanillin > syringaldehyde > furfural > 5-HMF > formic acid > levulinic acid > acetic acid. The NAPX37 strain showed substantial tolerance to typical inhibitors and showed good fermentation characteristics, when a medium with inhibitor cocktail or rape straw hydrolysate was used. This research provides important clues for inhibitors tolerance of recombinant industrial xylose-fermenting S. cerevisiae. |
topic |
Industrial Saccharomyces cerevisiae Bioethanol Glucose and xylose co-fermentation Inhibitor tolerance |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400791&lng=en&tlng=en |
work_keys_str_mv |
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1725582271578112000 |