Micromorphology, Ultrastructure and Histochemistry of <i>Commelina benghalensis</i> L. Leaves and Stems

• Main Authors: Kareshma Doolabh, Yougasphree Naidoo, Yaser Hassan Dewir, Nasser Al-Suhaibani
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: Plants
• Subjects: alkaloids; non-glandular trichomes; hooked; microscopy; morphology; multicellular
• Online Access: https://www.mdpi.com/2223-7747/10/3/512

<i>Commelina benghalensis</i> L. is used as a traditional medicine in treating numerous ailments and diseases such as infertility in women, conjunctivitis, gonorrhea, and jaundice. This study used light and electron microscopy coupled with histochemistry to investigate the micromorphology, ultrastructure and histochemical properties of <i>C. benghalensis </i>leaves and stems. Stereo and scanning electron microscopy revealed dense non-glandular trichomes on the leaves and stems and trichome density was greater in emergent leaves than in the young and mature. Three morphologically different non-glandular trichomes were observed including simple multicellular, simple bicellular and simple multicellular hooked. The simple bicellular trichomes were less common than the multicellular and hooked. Transmission electron micrographs showed mitochondria, vesicles and vacuoles in the trichome. The leaf section contained chloroplasts with plastoglobuli and starch grains. Histochemical analysis revealed various pharmacologically important compounds such as phenols, alkaloids, proteins and polysaccharides. The micromorphological and ultrastructural investigations suggest that <i>Commelina benghalensis</i> L. is an economically important medicinal plant due to bioactive compounds present in the leaves and stems.