Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
Abstract The expression of the channels-enzymes TRPM6 and TRPM7 in the human heart remains poorly defined, and TRPM6 is generally considered not to be expressed in cardiomyocytes. We examined their expression at protein and mRNA levels using right atrial samples resected from patients (n = 72) with...
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doaj-e0f6dda8283848018f01361c5601c6622021-08-01T11:23:07ZengNature Publishing GroupScientific Reports2045-23222021-07-0111111410.1038/s41598-021-94856-4Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heartInga Andriulė0Dalia Pangonytė1Mantė Almanaitytė2Vaiva Patamsytė3Milda Kuprytė4Dainius Karčiauskas5Kanigula Mubagwa6Regina Mačianskienė7Institute of Cardiology, Lithuanian University of Health SciencesInstitute of Cardiology, Lithuanian University of Health SciencesInstitute of Cardiology, Lithuanian University of Health SciencesInstitute of Cardiology, Lithuanian University of Health SciencesInstitute of Cardiology, Lithuanian University of Health SciencesDepartment of Cardiac, Thoracic and Vascular Surgery, Hospital of Lithuanian University of Health Sciences Kauno Klinikos, Lithuanian University of Health SciencesDepartment of Cardiovascular Sciences, Faculty of Medicine, KU LeuvenInstitute of Cardiology, Lithuanian University of Health SciencesAbstract The expression of the channels-enzymes TRPM6 and TRPM7 in the human heart remains poorly defined, and TRPM6 is generally considered not to be expressed in cardiomyocytes. We examined their expression at protein and mRNA levels using right atrial samples resected from patients (n = 72) with or without ischemic heart disease (IHD) and samples from all chamber walls of explanted human hearts (n = 9). TRPM6 and TRPM7 proteins were detected using immunofluorescence on isolated cardiomyocytes, ELISA on tissue homogenates, and immunostaining of cardiac tissue, whereas their mRNAs were detected by RT-qPCR. Both TRPM6 and TRPM7 were present in all chamber walls, with TRPM7 being more abundant. TRPM6 was co-expressed with TRPM7. The expression levels were dependent on cell incubation conditions (presence or absence of divalent cations, pH of the extracellular milieu, presence of TRP channel inhibitors 2-aminoethoxydiphenyl-borate and carvacrol). These drugs reduced TRPM7 immunofluorescence but increased that of TRPM6. TRPM6 and TRPM7 expression was increased in tissues from IHD patients. This is the first demonstration of the presence and co-expression of TRPM6 and TRPM7 in cardiomyocytes from all chamber walls of the human heart. The increased TRPM6 and TRPM7 expression in IHD suggests that the chanzymes are involved in the pathophysiology of the disease.https://doi.org/10.1038/s41598-021-94856-4 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Inga Andriulė Dalia Pangonytė Mantė Almanaitytė Vaiva Patamsytė Milda Kuprytė Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė |
spellingShingle |
Inga Andriulė Dalia Pangonytė Mantė Almanaitytė Vaiva Patamsytė Milda Kuprytė Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart Scientific Reports |
author_facet |
Inga Andriulė Dalia Pangonytė Mantė Almanaitytė Vaiva Patamsytė Milda Kuprytė Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė |
author_sort |
Inga Andriulė |
title |
Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart |
title_short |
Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart |
title_full |
Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart |
title_fullStr |
Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart |
title_full_unstemmed |
Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart |
title_sort |
evidence for the expression of trpm6 and trpm7 in cardiomyocytes from all four chamber walls of the human heart |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-07-01 |
description |
Abstract The expression of the channels-enzymes TRPM6 and TRPM7 in the human heart remains poorly defined, and TRPM6 is generally considered not to be expressed in cardiomyocytes. We examined their expression at protein and mRNA levels using right atrial samples resected from patients (n = 72) with or without ischemic heart disease (IHD) and samples from all chamber walls of explanted human hearts (n = 9). TRPM6 and TRPM7 proteins were detected using immunofluorescence on isolated cardiomyocytes, ELISA on tissue homogenates, and immunostaining of cardiac tissue, whereas their mRNAs were detected by RT-qPCR. Both TRPM6 and TRPM7 were present in all chamber walls, with TRPM7 being more abundant. TRPM6 was co-expressed with TRPM7. The expression levels were dependent on cell incubation conditions (presence or absence of divalent cations, pH of the extracellular milieu, presence of TRP channel inhibitors 2-aminoethoxydiphenyl-borate and carvacrol). These drugs reduced TRPM7 immunofluorescence but increased that of TRPM6. TRPM6 and TRPM7 expression was increased in tissues from IHD patients. This is the first demonstration of the presence and co-expression of TRPM6 and TRPM7 in cardiomyocytes from all chamber walls of the human heart. The increased TRPM6 and TRPM7 expression in IHD suggests that the chanzymes are involved in the pathophysiology of the disease. |
url |
https://doi.org/10.1038/s41598-021-94856-4 |
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