Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

Abstract Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots. The African cassava mosaic virus isolate from Nigeria (ACMV-[NOg]), which was initially cloned in a binar...

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Bibliographic Details
Main Authors: Ezequiel Matias Lentz, Joel-Elias Kuon, Adrian Alder, Nathalie Mangel, Ima M. Zainuddin, Emily Jane McCallum, Ravi Bodampalli Anjanappa, Wilhelm Gruissem, Hervé Vanderschuren
Format: Article
Language:English
Published: BMC 2018-08-01
Series:Plant Methods
Subjects:
Cassava
VIGS
ACMV
Geminivirus
Agrobacterium tumefaciens
Gene silencing
Online Access:http://link.springer.com/article/10.1186/s13007-018-0340-5

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http://link.springer.com/article/10.1186/s13007-018-0340-5

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