Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
Plants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very...
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doaj-e11156af69af42c9bf776bef1459659a2020-11-25T02:03:34ZengMDPI AGPlants2223-77472020-02-019226310.3390/plants9020263plants9020263Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected FieldsManoj Kaushal0George Mahuku1Rony Swennen2International Institute of Tropical Agriculture (IITA), Mikocheni B, Dar es Salaam-34441, TanzaniaInternational Institute of Tropical Agriculture (IITA), Mikocheni B, Dar es Salaam-34441, TanzaniaBioversity International, Willem De Croylaan 42, B-3001 Leuven, BelgiumPlants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very recently, and their potential role in disease development has not been explored so far. Roots from symptomatic and non-symptomatic banana plants were sampled from fields infected by <i>Fusarium oxysporum</i> f.sp. <i>cubense</i> race 1. The goal was to compare the endophytic microbiota between symptomatic and non-symptomatic plants through high throughput sequencing of 16s rDNA and shotgun metagenome sequencing. The results revealed that the endophytic root microbiome in bananas is dominated by Proteobacteria and Bacteroidetes followed to a lesser extent by Actinobacteria. The development of disease greatly impacted the endophytic microbial communities. For example, Flavobacteriales abundance was correlated with symptom development.https://www.mdpi.com/2223-7747/9/2/263bananamicrobial diversityrhizospheremetagenomics<i>fusarium oxysporum</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manoj Kaushal George Mahuku Rony Swennen |
spellingShingle |
Manoj Kaushal George Mahuku Rony Swennen Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields Plants banana microbial diversity rhizosphere metagenomics <i>fusarium oxysporum</i> |
author_facet |
Manoj Kaushal George Mahuku Rony Swennen |
author_sort |
Manoj Kaushal |
title |
Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields |
title_short |
Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields |
title_full |
Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields |
title_fullStr |
Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields |
title_full_unstemmed |
Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields |
title_sort |
metagenomic insights of the root colonizing microbiome associated with symptomatic and non-symptomatic bananas in <i>fusarium</i> wilt infected fields |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-02-01 |
description |
Plants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very recently, and their potential role in disease development has not been explored so far. Roots from symptomatic and non-symptomatic banana plants were sampled from fields infected by <i>Fusarium oxysporum</i> f.sp. <i>cubense</i> race 1. The goal was to compare the endophytic microbiota between symptomatic and non-symptomatic plants through high throughput sequencing of 16s rDNA and shotgun metagenome sequencing. The results revealed that the endophytic root microbiome in bananas is dominated by Proteobacteria and Bacteroidetes followed to a lesser extent by Actinobacteria. The development of disease greatly impacted the endophytic microbial communities. For example, Flavobacteriales abundance was correlated with symptom development. |
topic |
banana microbial diversity rhizosphere metagenomics <i>fusarium oxysporum</i> |
url |
https://www.mdpi.com/2223-7747/9/2/263 |
work_keys_str_mv |
AT manojkaushal metagenomicinsightsoftherootcolonizingmicrobiomeassociatedwithsymptomaticandnonsymptomaticbananasinifusariumiwiltinfectedfields AT georgemahuku metagenomicinsightsoftherootcolonizingmicrobiomeassociatedwithsymptomaticandnonsymptomaticbananasinifusariumiwiltinfectedfields AT ronyswennen metagenomicinsightsoftherootcolonizingmicrobiomeassociatedwithsymptomaticandnonsymptomaticbananasinifusariumiwiltinfectedfields |
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