Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields

Plants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very...

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Main Authors: Manoj Kaushal, George Mahuku, Rony Swennen
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Plants
Subjects:
Online Access:https://www.mdpi.com/2223-7747/9/2/263
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spelling doaj-e11156af69af42c9bf776bef1459659a2020-11-25T02:03:34ZengMDPI AGPlants2223-77472020-02-019226310.3390/plants9020263plants9020263Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected FieldsManoj Kaushal0George Mahuku1Rony Swennen2International Institute of Tropical Agriculture (IITA), Mikocheni B, Dar es Salaam-34441, TanzaniaInternational Institute of Tropical Agriculture (IITA), Mikocheni B, Dar es Salaam-34441, TanzaniaBioversity International, Willem De Croylaan 42, B-3001 Leuven, BelgiumPlants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very recently, and their potential role in disease development has not been explored so far. Roots from symptomatic and non-symptomatic banana plants were sampled from fields infected by <i>Fusarium oxysporum</i> f.sp. <i>cubense</i> race 1. The goal was to compare the endophytic microbiota between symptomatic and non-symptomatic plants through high throughput sequencing of 16s rDNA and shotgun metagenome sequencing. The results revealed that the endophytic root microbiome in bananas is dominated by Proteobacteria and Bacteroidetes followed to a lesser extent by Actinobacteria. The development of disease greatly impacted the endophytic microbial communities. For example, Flavobacteriales abundance was correlated with symptom development.https://www.mdpi.com/2223-7747/9/2/263bananamicrobial diversityrhizospheremetagenomics<i>fusarium oxysporum</i>
collection DOAJ
language English
format Article
sources DOAJ
author Manoj Kaushal
George Mahuku
Rony Swennen
spellingShingle Manoj Kaushal
George Mahuku
Rony Swennen
Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
Plants
banana
microbial diversity
rhizosphere
metagenomics
<i>fusarium oxysporum</i>
author_facet Manoj Kaushal
George Mahuku
Rony Swennen
author_sort Manoj Kaushal
title Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
title_short Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
title_full Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
title_fullStr Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
title_full_unstemmed Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in <i>Fusarium</i> Wilt Infected Fields
title_sort metagenomic insights of the root colonizing microbiome associated with symptomatic and non-symptomatic bananas in <i>fusarium</i> wilt infected fields
publisher MDPI AG
series Plants
issn 2223-7747
publishDate 2020-02-01
description Plants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very recently, and their potential role in disease development has not been explored so far. Roots from symptomatic and non-symptomatic banana plants were sampled from fields infected by <i>Fusarium oxysporum</i> f.sp. <i>cubense</i> race 1. The goal was to compare the endophytic microbiota between symptomatic and non-symptomatic plants through high throughput sequencing of 16s rDNA and shotgun metagenome sequencing. The results revealed that the endophytic root microbiome in bananas is dominated by Proteobacteria and Bacteroidetes followed to a lesser extent by Actinobacteria. The development of disease greatly impacted the endophytic microbial communities. For example, Flavobacteriales abundance was correlated with symptom development.
topic banana
microbial diversity
rhizosphere
metagenomics
<i>fusarium oxysporum</i>
url https://www.mdpi.com/2223-7747/9/2/263
work_keys_str_mv AT manojkaushal metagenomicinsightsoftherootcolonizingmicrobiomeassociatedwithsymptomaticandnonsymptomaticbananasinifusariumiwiltinfectedfields
AT georgemahuku metagenomicinsightsoftherootcolonizingmicrobiomeassociatedwithsymptomaticandnonsymptomaticbananasinifusariumiwiltinfectedfields
AT ronyswennen metagenomicinsightsoftherootcolonizingmicrobiomeassociatedwithsymptomaticandnonsymptomaticbananasinifusariumiwiltinfectedfields
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