Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i>
The present study describes the production of the value-added chemical 1,3-propanediol (1,3-PD) from crude glycerol, a waste by-product formed during biodiesel production. The efficiency, robustness, and stability of the process were improved by immobilization of the anaerobic bacterium <i>Clo...
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doaj-e1199da47d29486cbf3de2375743391a2020-11-24T21:52:47ZengMDPI AGCatalysts2073-43442019-03-019431710.3390/catal9040317catal9040317Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i>Igor Dolejš0Monika Líšková1Vladimír Krasňan2Kristína Markošová3Michal Rosenberg4Fabio Lorenzini5Andrew C. Marr6Martin Rebroš7Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaSchool of Chemistry and Chemical Engineering, Queen’s University Belfast, Belfast BT9 5AG, UKSchool of Chemistry and Chemical Engineering, Queen’s University Belfast, Belfast BT9 5AG, UKInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaThe present study describes the production of the value-added chemical 1,3-propanediol (1,3-PD) from crude glycerol, a waste by-product formed during biodiesel production. The efficiency, robustness, and stability of the process were improved by immobilization of the anaerobic bacterium <i>Clostridium butyricum</i> into a polyvinyl alcohol (PVA) hydrogel. The highest average productivity, 6.8 ± 0.2 g/(L·h), was achieved in 10 consecutive, repeated batch fermentations, with an initial concentration of pure glycerol 45.5 ± 0.7 g/L, after 2.5 h. The highest final concentration and yield of 1,3-PD, 28.3 ± 0.6 g/L, and 0.42 ± 0.01 g/g, respectively, were achieved in eleven repeated batch fermentations, after increasing the initial pure glycerol concentration to 70.4 ± 1.9 g/L. Two different types of crude glycerol, produced from used cooking oil (UCO) and rapeseed oil (RO), were tested in repeated batch fermentations, with an average productivity achieved of 2.3 ± 0.1 and 3.5 ± 0.3 g/(L·h), respectively. The highest final concentration and yield of 1,3-PD, 12.6 ± 0.9 g/L, and 0.35 ± 0.02 g/g, respectively, were observed in fifteen repeated batch fermentations with RO crude glycerol. An excellent stability of the immobilized anaerobic bacteria and increase of productivity in fermentation of crude glycerol was demonstrated.https://www.mdpi.com/2073-4344/9/4/317<i>Clostridium butyricum</i>immobilization1,3-propanediolcrude glycerol |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Igor Dolejš Monika Líšková Vladimír Krasňan Kristína Markošová Michal Rosenberg Fabio Lorenzini Andrew C. Marr Martin Rebroš |
spellingShingle |
Igor Dolejš Monika Líšková Vladimír Krasňan Kristína Markošová Michal Rosenberg Fabio Lorenzini Andrew C. Marr Martin Rebroš Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i> Catalysts <i>Clostridium butyricum</i> immobilization 1,3-propanediol crude glycerol |
author_facet |
Igor Dolejš Monika Líšková Vladimír Krasňan Kristína Markošová Michal Rosenberg Fabio Lorenzini Andrew C. Marr Martin Rebroš |
author_sort |
Igor Dolejš |
title |
Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i> |
title_short |
Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i> |
title_full |
Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i> |
title_fullStr |
Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i> |
title_full_unstemmed |
Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized <i>Clostridium butyricum</i> |
title_sort |
production of 1,3-propanediol from pure and crude glycerol using immobilized <i>clostridium butyricum</i> |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2019-03-01 |
description |
The present study describes the production of the value-added chemical 1,3-propanediol (1,3-PD) from crude glycerol, a waste by-product formed during biodiesel production. The efficiency, robustness, and stability of the process were improved by immobilization of the anaerobic bacterium <i>Clostridium butyricum</i> into a polyvinyl alcohol (PVA) hydrogel. The highest average productivity, 6.8 ± 0.2 g/(L·h), was achieved in 10 consecutive, repeated batch fermentations, with an initial concentration of pure glycerol 45.5 ± 0.7 g/L, after 2.5 h. The highest final concentration and yield of 1,3-PD, 28.3 ± 0.6 g/L, and 0.42 ± 0.01 g/g, respectively, were achieved in eleven repeated batch fermentations, after increasing the initial pure glycerol concentration to 70.4 ± 1.9 g/L. Two different types of crude glycerol, produced from used cooking oil (UCO) and rapeseed oil (RO), were tested in repeated batch fermentations, with an average productivity achieved of 2.3 ± 0.1 and 3.5 ± 0.3 g/(L·h), respectively. The highest final concentration and yield of 1,3-PD, 12.6 ± 0.9 g/L, and 0.35 ± 0.02 g/g, respectively, were observed in fifteen repeated batch fermentations with RO crude glycerol. An excellent stability of the immobilized anaerobic bacteria and increase of productivity in fermentation of crude glycerol was demonstrated. |
topic |
<i>Clostridium butyricum</i> immobilization 1,3-propanediol crude glycerol |
url |
https://www.mdpi.com/2073-4344/9/4/317 |
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