A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA
Eastern oyster (<i>Crassostrea virginica</i>) aquaculture is increasingly playing a significant role in the state of Maine’s (USA) coastal economy. Here, we conducted a qPCR-based survey for <i>Haplosporidium nelsoni</i>, <i>Perkinsus marinus</i>, and <i>Per...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-03-01
|
Series: | Pathogens |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-0817/9/4/256 |
id |
doaj-e12e2eaa4aae4836acb4515ffbbfec95 |
---|---|
record_format |
Article |
spelling |
doaj-e12e2eaa4aae4836acb4515ffbbfec952020-11-25T02:09:22ZengMDPI AGPathogens2076-08172020-03-01925625610.3390/pathogens9040256A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USANicholas D. Marquis0Theodore J. Bishop1Nicholas R. Record2Peter D. Countway3José A. Fernández Robledo4Bigelow Laboratory for Ocean Sciences, Boothbay, ME 04544, USABigelow Laboratory for Ocean Sciences, Boothbay, ME 04544, USABigelow Laboratory for Ocean Sciences, Boothbay, ME 04544, USABigelow Laboratory for Ocean Sciences, Boothbay, ME 04544, USABigelow Laboratory for Ocean Sciences, Boothbay, ME 04544, USAEastern oyster (<i>Crassostrea virginica</i>) aquaculture is increasingly playing a significant role in the state of Maine’s (USA) coastal economy. Here, we conducted a qPCR-based survey for <i>Haplosporidium nelsoni</i>, <i>Perkinsus marinus</i>, and <i>Perkinsus chesapeaki</i> in <i>C. virginica</i> (n = 1440) from six Maine sites during the summer–fall of 2016 and 2017. In the absence of reported die-offs, our results indicated the continued presence of the three protozoan parasites in the six sites. The highest <i>H. nelsoni</i> qPCR-prevalence corresponded to Jack’s Point and Prentiss Island (<inline-formula> <math display="inline"> <semantics> <mrow> <mover accent="true"> <mi>x</mi> <mo>¯</mo> </mover> <mo>=</mo> </mrow> </semantics> </math> </inline-formula> 40 and 48% respectively), both located in the Damariscotta River Estuary. Jack’s Point, Prentiss Island, New Meadows River, and Weskeag River recorded the highest qPCR-prevalence for <i>P. marinus</i> (32–39%). While the <i>P. marinus</i> qPCR-prevalence differed slightly for the years 2016 and 2017, <i>P. chesapeaki</i> qPCR-prevalence in 2016 was markedly lower than 2017 (<20% at all sites versus >60% at all sites for each of the years, respectively). Mean qPCR-prevalence values for <i>P. chesapeaki</i> over the two-year study were ≥40% for samples from Jack’s Point (49%), Prentiss Island (44%), and New Meadows River (40%). This study highlights that large and sustained surveys for parasitic diseases are fundamental for decision making toward the management of the shellfish aquaculture industry, especially for having a baseline in the case that die-offs occur.https://www.mdpi.com/2076-0817/9/4/256AlveolateAscetosporabivalveshost-parasite interactionparasite association |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicholas D. Marquis Theodore J. Bishop Nicholas R. Record Peter D. Countway José A. Fernández Robledo |
spellingShingle |
Nicholas D. Marquis Theodore J. Bishop Nicholas R. Record Peter D. Countway José A. Fernández Robledo A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA Pathogens Alveolate Ascetospora bivalves host-parasite interaction parasite association |
author_facet |
Nicholas D. Marquis Theodore J. Bishop Nicholas R. Record Peter D. Countway José A. Fernández Robledo |
author_sort |
Nicholas D. Marquis |
title |
A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA |
title_short |
A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA |
title_full |
A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA |
title_fullStr |
A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA |
title_full_unstemmed |
A qPCR-Based Survey of <i>Haplosporidium nelsoni</i> and <i>Perkinsus</i> spp. in the Eastern Oyster, <i>Crassostrea virginica</i> in Maine, USA |
title_sort |
qpcr-based survey of <i>haplosporidium nelsoni</i> and <i>perkinsus</i> spp. in the eastern oyster, <i>crassostrea virginica</i> in maine, usa |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2020-03-01 |
description |
Eastern oyster (<i>Crassostrea virginica</i>) aquaculture is increasingly playing a significant role in the state of Maine’s (USA) coastal economy. Here, we conducted a qPCR-based survey for <i>Haplosporidium nelsoni</i>, <i>Perkinsus marinus</i>, and <i>Perkinsus chesapeaki</i> in <i>C. virginica</i> (n = 1440) from six Maine sites during the summer–fall of 2016 and 2017. In the absence of reported die-offs, our results indicated the continued presence of the three protozoan parasites in the six sites. The highest <i>H. nelsoni</i> qPCR-prevalence corresponded to Jack’s Point and Prentiss Island (<inline-formula> <math display="inline"> <semantics> <mrow> <mover accent="true"> <mi>x</mi> <mo>¯</mo> </mover> <mo>=</mo> </mrow> </semantics> </math> </inline-formula> 40 and 48% respectively), both located in the Damariscotta River Estuary. Jack’s Point, Prentiss Island, New Meadows River, and Weskeag River recorded the highest qPCR-prevalence for <i>P. marinus</i> (32–39%). While the <i>P. marinus</i> qPCR-prevalence differed slightly for the years 2016 and 2017, <i>P. chesapeaki</i> qPCR-prevalence in 2016 was markedly lower than 2017 (<20% at all sites versus >60% at all sites for each of the years, respectively). Mean qPCR-prevalence values for <i>P. chesapeaki</i> over the two-year study were ≥40% for samples from Jack’s Point (49%), Prentiss Island (44%), and New Meadows River (40%). This study highlights that large and sustained surveys for parasitic diseases are fundamental for decision making toward the management of the shellfish aquaculture industry, especially for having a baseline in the case that die-offs occur. |
topic |
Alveolate Ascetospora bivalves host-parasite interaction parasite association |
url |
https://www.mdpi.com/2076-0817/9/4/256 |
work_keys_str_mv |
AT nicholasdmarquis aqpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT theodorejbishop aqpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT nicholasrrecord aqpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT peterdcountway aqpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT joseafernandezrobledo aqpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT nicholasdmarquis qpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT theodorejbishop qpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT nicholasrrecord qpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT peterdcountway qpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa AT joseafernandezrobledo qpcrbasedsurveyofihaplosporidiumnelsoniiandiperkinsusisppintheeasternoystericrassostreavirginicaiinmaineusa |
_version_ |
1724924347597979648 |