Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells
Abstract Src-family kinases, expressed in a wide variety of cell types, are anchored to cellular membranes through posttranslational lipid modifications and involved in diverse cellular signalling. In epithelial cells, Src-family kinases are localized at the plasma membrane and participate in epithe...
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doaj-e131173f728d45ccabceeab80ba8066a2020-12-08T02:14:55ZengNature Publishing GroupScientific Reports2045-23222017-03-017111310.1038/s41598-017-00538-5Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cellsTakao Morinaga0Sayuri Yanase1Aya Okamoto2Noritaka Yamaguchi3Naoto Yamaguchi4Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba UniversityLaboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba UniversityLaboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba UniversityLaboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba UniversityLaboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba UniversityAbstract Src-family kinases, expressed in a wide variety of cell types, are anchored to cellular membranes through posttranslational lipid modifications and involved in diverse cellular signalling. In epithelial cells, Src-family kinases are localized at the plasma membrane and participate in epithelial functions. Epithelial cell polarity is achieved through dynamic reorganization of protein trafficking. To examine the trafficking of Src-family kinases between polarized and non-polarized epithelial cells, we generated an MDCK cell line that can inducibly express a protein of interest in a polarized state at any time. We show here that Lyn, a member of Src-family kinases, mainly localizes to the plasma membrane in polarized MDCK cells and to endomembranes in non-polarized MDCK cells. Cell-cell interactions between adjacent MDCK cells recruit Lyn from endomembranes to the plasma membrane even without cell attachment to extracellular matrix scaffolds, and loss of cell-cell interactions by calcium deprivation relocates Lyn from the plasma membrane to endomembranes through Rab11-mediated recycling. Therefore, using our MDCK cells expressing inducible Lyn, we reveal that calcium-dependent cell-cell interactions play a critical role in plasma membrane localization of Lyn in polarized MDCK cells.https://doi.org/10.1038/s41598-017-00538-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takao Morinaga Sayuri Yanase Aya Okamoto Noritaka Yamaguchi Naoto Yamaguchi |
spellingShingle |
Takao Morinaga Sayuri Yanase Aya Okamoto Noritaka Yamaguchi Naoto Yamaguchi Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells Scientific Reports |
author_facet |
Takao Morinaga Sayuri Yanase Aya Okamoto Noritaka Yamaguchi Naoto Yamaguchi |
author_sort |
Takao Morinaga |
title |
Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells |
title_short |
Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells |
title_full |
Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells |
title_fullStr |
Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells |
title_full_unstemmed |
Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells |
title_sort |
recruitment of lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible lyn-expressing mdck cells |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-03-01 |
description |
Abstract Src-family kinases, expressed in a wide variety of cell types, are anchored to cellular membranes through posttranslational lipid modifications and involved in diverse cellular signalling. In epithelial cells, Src-family kinases are localized at the plasma membrane and participate in epithelial functions. Epithelial cell polarity is achieved through dynamic reorganization of protein trafficking. To examine the trafficking of Src-family kinases between polarized and non-polarized epithelial cells, we generated an MDCK cell line that can inducibly express a protein of interest in a polarized state at any time. We show here that Lyn, a member of Src-family kinases, mainly localizes to the plasma membrane in polarized MDCK cells and to endomembranes in non-polarized MDCK cells. Cell-cell interactions between adjacent MDCK cells recruit Lyn from endomembranes to the plasma membrane even without cell attachment to extracellular matrix scaffolds, and loss of cell-cell interactions by calcium deprivation relocates Lyn from the plasma membrane to endomembranes through Rab11-mediated recycling. Therefore, using our MDCK cells expressing inducible Lyn, we reveal that calcium-dependent cell-cell interactions play a critical role in plasma membrane localization of Lyn in polarized MDCK cells. |
url |
https://doi.org/10.1038/s41598-017-00538-5 |
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