YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
Abstract The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the prot...
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2017-08-01
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doaj-e1454c1049444b1085e58a8a95fe73ff2020-12-08T00:39:44ZengNature Publishing GroupScientific Reports2045-23222017-08-017111110.1038/s41598-017-09124-1YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of LactobacillusLucía Brown0Josefina M. Villegas1Mariano Elean2Silvina Fadda3Fernanda Mozzi4Lucila Saavedra5Elvira M. Hebert6Centro de Referencia para Lactobacilos (CERELA-CONICET)Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNTCentro de Referencia para Lactobacilos (CERELA-CONICET)Centro de Referencia para Lactobacilos (CERELA-CONICET)Centro de Referencia para Lactobacilos (CERELA-CONICET)Centro de Referencia para Lactobacilos (CERELA-CONICET)Centro de Referencia para Lactobacilos (CERELA-CONICET)Abstract The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp. lactis CRL 581 was performed. Genes encoding the cell envelope-associated proteinase, two peptide transport systems, and sixteen peptidases were identified. The influence of the peptide supply on the transcription of 23 genes involved in the proteolytic system of L. delbrueckii subsp. lactis was examined after cell growth in a chemically defined medium (CDM) and CDM supplemented with Casitone. prtL, oppA 1, optS, optA genes as well as oppDFBC and optBCDF operons were the most highly expressed genes in CDM; their expression being repressed 6- to 115-fold by the addition of peptides. The transcriptional analysis was confirmed by proteomics; the up-regulation of the PrtL, PepG, OppD and OptF proteins in the absence of peptides was observed while the DNA-binding protein YebC was up-regulated by peptides. Binding of YebC to the promoter region of prtL, oppA 1, and optS, demonstrated by electrophoretic mobility shift assays, showed that YebC acts as a transcriptional repressor of key proteolytic genes.https://doi.org/10.1038/s41598-017-09124-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lucía Brown Josefina M. Villegas Mariano Elean Silvina Fadda Fernanda Mozzi Lucila Saavedra Elvira M. Hebert |
spellingShingle |
Lucía Brown Josefina M. Villegas Mariano Elean Silvina Fadda Fernanda Mozzi Lucila Saavedra Elvira M. Hebert YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus Scientific Reports |
author_facet |
Lucía Brown Josefina M. Villegas Mariano Elean Silvina Fadda Fernanda Mozzi Lucila Saavedra Elvira M. Hebert |
author_sort |
Lucía Brown |
title |
YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus |
title_short |
YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus |
title_full |
YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus |
title_fullStr |
YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus |
title_full_unstemmed |
YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus |
title_sort |
yebc, a putative transcriptional factor involved in the regulation of the proteolytic system of lactobacillus |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp. lactis CRL 581 was performed. Genes encoding the cell envelope-associated proteinase, two peptide transport systems, and sixteen peptidases were identified. The influence of the peptide supply on the transcription of 23 genes involved in the proteolytic system of L. delbrueckii subsp. lactis was examined after cell growth in a chemically defined medium (CDM) and CDM supplemented with Casitone. prtL, oppA 1, optS, optA genes as well as oppDFBC and optBCDF operons were the most highly expressed genes in CDM; their expression being repressed 6- to 115-fold by the addition of peptides. The transcriptional analysis was confirmed by proteomics; the up-regulation of the PrtL, PepG, OppD and OptF proteins in the absence of peptides was observed while the DNA-binding protein YebC was up-regulated by peptides. Binding of YebC to the promoter region of prtL, oppA 1, and optS, demonstrated by electrophoretic mobility shift assays, showed that YebC acts as a transcriptional repressor of key proteolytic genes. |
url |
https://doi.org/10.1038/s41598-017-09124-1 |
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