Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization

Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization

Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate...

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Main Authors: DERI YURATMOKO, NISA RACHMANIA MUBARIK, ANJA MERYANDINI
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2010-08-01
Series:Microbiology Indonesia
Subjects:
Streptomyces sp.
protease
keratinase
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/63
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spelling doaj-e15ec1710fec4671a5ece3777662a9c32021-08-31T13:00:38ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752010-08-011210.5454/mi.1.2.559Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their CharacterizationDERI YURATMOKONISA RACHMANIA MUBARIKANJA MERYANDINIProtease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 ºC, whereas that of the keratinase was at pH 6.5 and 70 +C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 ºC whereas the keratinase was at pH 8.5 and 80 ºC.https://jurnal.permi.or.id/index.php/mionline/article/view/63Streptomyces sp.proteasekeratinase
collection DOAJ
language English
format Article
sources DOAJ
author DERI YURATMOKO
NISA RACHMANIA MUBARIK
ANJA MERYANDINI
spellingShingle DERI YURATMOKO
NISA RACHMANIA MUBARIK
ANJA MERYANDINI
Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
Microbiology Indonesia
Streptomyces sp.
protease
keratinase
author_facet DERI YURATMOKO
NISA RACHMANIA MUBARIK
ANJA MERYANDINI
author_sort DERI YURATMOKO
title Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
title_short Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
title_full Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
title_fullStr Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
title_full_unstemmed Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
title_sort screening of proteolytic enzymes of streptomyces sp. local strain and their characterization
publisher Indonesian Society for Microbiology
series Microbiology Indonesia
issn 1978-3477
2087-8575
publishDate 2010-08-01
description Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 ºC, whereas that of the keratinase was at pH 6.5 and 70 +C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 ºC whereas the keratinase was at pH 8.5 and 80 ºC.
topic Streptomyces sp.
protease
keratinase
url https://jurnal.permi.or.id/index.php/mionline/article/view/63
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AT nisarachmaniamubarik screeningofproteolyticenzymesofstreptomycessplocalstrainandtheircharacterization
AT anjameryandini screeningofproteolyticenzymesofstreptomycessplocalstrainandtheircharacterization
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