Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy

• Main Authors: Qingxue Liu, Lei Xu, Meifei Wu, Yiwen Zhou, Junfa Yang, Cheng Huang, Tao Xu, Jun Li, Lei Zhang
• Format: Article
• Language: English
• Published: BMC 2021-07-01
• Series: Cell & Bioscience
• Subjects: Rev-erbα; AFL; Autophagy; Bmal1; Lysosome
• Online Access: https://doi.org/10.1186/s13578-021-00622-4

Abstract Background and aims Alcoholic fatty liver (AFL) is a liver disease caused by long-term excessive drinking and is characterized by hepatic steatosis. Understanding the regulatory mechanism of steatosis is essential for the treatment of AFL. Rev-erbα is a member of the Rev-erbs family of nuclear receptors, playing an important role in regulating lipid metabolism. However, its functional role in AFL and its underlying mechanism remains unclear. Results Rev-erbα was upregulated in the liver of EtOH-fed mice and EtOH-treated L-02 cells. Further, Rev-erbα activation exacerbates steatosis in L-02 cells. Inhibition/downexpression of Rev-erbα improved steatosis. Mechanistically, autophagy activity was inhibited in vivo and vitro. Interestingly, inhibition/downexpression of Rev-erbα enhanced autophagy. Furthermore, silencing of Rev-erbα up-regulated the nuclear expression of Bmal1. Autophagy activity was inhibited and steatosis was deteriorated after EtOH-treated L-02 cells were cotransfected with Rev-erbα shRNA and Bmal1 siRNA. Conclusions Rev-erbα induces liver steatosis, which promotes the progression of AFL. Our study reveals a novel steatosis regulatory mechanism in AFL and suggest that Rev-erbα might be a potential therapeutic target for AFL.