<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation
Mosquitoes inject saliva into the host skin to facilitate blood meal acquisition through active compounds that prevent hemostasis. D7 proteins are among the most abundant components of the mosquito saliva and act as scavengers of biogenic amines and eicosanoids. Several members of the D7 family have...
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doaj-e1954158faad4eedb6e17e9906d49b1b2020-11-25T02:44:57ZengMDPI AGBiomolecules2218-273X2020-09-01101372137210.3390/biom10101372<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and InflammationInes Martin-Martin0Leticia Barion Smith1Andrezza Campos Chagas2Anderson Sa Nunes3Gaurav Shrivastava4Paola Carolina Valenzuela-Leon5Eric Calvo6Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USALaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USALaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USADepartment of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo 05508-000, BrazilLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USALaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USALaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USAMosquitoes inject saliva into the host skin to facilitate blood meal acquisition through active compounds that prevent hemostasis. D7 proteins are among the most abundant components of the mosquito saliva and act as scavengers of biogenic amines and eicosanoids. Several members of the D7 family have been characterized at the biochemical level; however, none have been studied thus far in <i>Aedes albopictus</i>, a permissive vector for several arboviruses that causes extensive human morbidity and mortality. Here, we report the binding capabilities of a D7 long form protein from <i>Ae. albopictus</i> (AlboD7L1) by isothermal titration calorimetry and compared its model structure with previously solved D7 structures. The physiological function of AlboD7L1 was demonstrated by ex vivo platelet aggregation and in vivo leukocyte recruitment experiments. AlboD7L1 binds host hemostasis agonists, including biogenic amines, leukotrienes, and the thromboxane A2 analog U-46619. AlboD7L1 protein model predicts binding of biolipids through its N-terminal domain, while the C-terminal domain binds biogenic amines. We demonstrated the biological function of AlboD7L1 as an inhibitor of both platelet aggregation and cell recruitment of neutrophils and eosinophils. Altogether, this study reinforces the physiological relevance of the D7 salivary proteins as anti-hemostatic and anti-inflammatory molecules that help blood feeding in mosquitoes.https://www.mdpi.com/2218-273X/10/10/1372mosquitoD7 proteinsarthropodssalivary glandssalivaisothermal titration calorimetry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ines Martin-Martin Leticia Barion Smith Andrezza Campos Chagas Anderson Sa Nunes Gaurav Shrivastava Paola Carolina Valenzuela-Leon Eric Calvo |
spellingShingle |
Ines Martin-Martin Leticia Barion Smith Andrezza Campos Chagas Anderson Sa Nunes Gaurav Shrivastava Paola Carolina Valenzuela-Leon Eric Calvo <i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation Biomolecules mosquito D7 proteins arthropods salivary glands saliva isothermal titration calorimetry |
author_facet |
Ines Martin-Martin Leticia Barion Smith Andrezza Campos Chagas Anderson Sa Nunes Gaurav Shrivastava Paola Carolina Valenzuela-Leon Eric Calvo |
author_sort |
Ines Martin-Martin |
title |
<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation |
title_short |
<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation |
title_full |
<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation |
title_fullStr |
<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation |
title_full_unstemmed |
<i>Aedes albopictus</i> D7 Salivary Protein Prevents Host Hemostasis and Inflammation |
title_sort |
<i>aedes albopictus</i> d7 salivary protein prevents host hemostasis and inflammation |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2020-09-01 |
description |
Mosquitoes inject saliva into the host skin to facilitate blood meal acquisition through active compounds that prevent hemostasis. D7 proteins are among the most abundant components of the mosquito saliva and act as scavengers of biogenic amines and eicosanoids. Several members of the D7 family have been characterized at the biochemical level; however, none have been studied thus far in <i>Aedes albopictus</i>, a permissive vector for several arboviruses that causes extensive human morbidity and mortality. Here, we report the binding capabilities of a D7 long form protein from <i>Ae. albopictus</i> (AlboD7L1) by isothermal titration calorimetry and compared its model structure with previously solved D7 structures. The physiological function of AlboD7L1 was demonstrated by ex vivo platelet aggregation and in vivo leukocyte recruitment experiments. AlboD7L1 binds host hemostasis agonists, including biogenic amines, leukotrienes, and the thromboxane A2 analog U-46619. AlboD7L1 protein model predicts binding of biolipids through its N-terminal domain, while the C-terminal domain binds biogenic amines. We demonstrated the biological function of AlboD7L1 as an inhibitor of both platelet aggregation and cell recruitment of neutrophils and eosinophils. Altogether, this study reinforces the physiological relevance of the D7 salivary proteins as anti-hemostatic and anti-inflammatory molecules that help blood feeding in mosquitoes. |
topic |
mosquito D7 proteins arthropods salivary glands saliva isothermal titration calorimetry |
url |
https://www.mdpi.com/2218-273X/10/10/1372 |
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