Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin
The biomineralisation of radicular dentin involves complex molecular signalling. Providing evidence of protein binding sites for calcium ions and mineral precipitation is essential for a better understanding of the remineralisation process. This study aimed to evaluate the functional relationship of...
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doaj-e1a02c965b8a4c59aa194a1d830f2d372020-11-25T00:36:54ZengMDPI AGMaterials1996-19442020-02-01135105310.3390/ma13051053ma13051053Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular DentinCristina Retana-Lobo0Juliane Maria Guerreiro-Tanomaru1Mario Tanomaru-Filho2Beatriz Dulcineia Mendes de Souza3Jessie Reyes-Carmona4LICIFO—Laboratory of Research in Dental Sciences, Department of Endodontics, Faculty of Dentistry, University of Costa Rica, San Jose 11502 SJO, Costa RicaDepartment of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP 14801385, BrazilDepartment of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP 14801385, BrazilDepartment of Dentistry, Federal University of Santa Catarina, Florianopolis, SC 88040-900, BrazilLICIFO—Laboratory of Research in Dental Sciences, Department of Endodontics, Faculty of Dentistry, University of Costa Rica, San Jose 11502 SJO, Costa RicaThe biomineralisation of radicular dentin involves complex molecular signalling. Providing evidence of protein binding sites for calcium ions and mineral precipitation is essential for a better understanding of the remineralisation process. This study aimed to evaluate the functional relationship of metalloproteinases (MMPs) and non-collagenous proteins (NCPs) with mineral initiation and maturation during the biomineralisation of radicular dentin. A standardized demineralisation procedure was performed to radicular dentin slices. Samples were remineralised in a PBS-bioactive material system for different periods of time. Assessments of ion exchange, Raman analysis, and energy dispersive X-ray analysis (EDAX) with a scanning electron microscope (SEM) were used to evaluate the remineralisation process. Immunohistochemistry and zymography were performed to analyse NCPs and MMPs expression. SEM evaluation showed that the mineral nucleation and growth occurs, exclusively, on the demineralised radicular dentin surface. Raman analysis of remineralised dentin showed intense peaks at 955 and 1063 cm<sup>−1</sup>, which can be attributed to carbonate apatite formation. Immunohistochemistry of demineralised samples revealed the presence of DMP1-CT, mainly in intratubular dentin, whereas DSPP in intratubular and intertubular dentin. DMP1-CT and DSPP binding sites control carbonate apatite nucleation and maturation guiding the remineralisation of radicular dentin.https://www.mdpi.com/1996-1944/13/5/1053biomineralisationcarbonate apatitebioactive materialsdentin remineralizationmatrix metalloproteinasesnon-collagenous proteinsradicular dentin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristina Retana-Lobo Juliane Maria Guerreiro-Tanomaru Mario Tanomaru-Filho Beatriz Dulcineia Mendes de Souza Jessie Reyes-Carmona |
spellingShingle |
Cristina Retana-Lobo Juliane Maria Guerreiro-Tanomaru Mario Tanomaru-Filho Beatriz Dulcineia Mendes de Souza Jessie Reyes-Carmona Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin Materials biomineralisation carbonate apatite bioactive materials dentin remineralization matrix metalloproteinases non-collagenous proteins radicular dentin |
author_facet |
Cristina Retana-Lobo Juliane Maria Guerreiro-Tanomaru Mario Tanomaru-Filho Beatriz Dulcineia Mendes de Souza Jessie Reyes-Carmona |
author_sort |
Cristina Retana-Lobo |
title |
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin |
title_short |
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin |
title_full |
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin |
title_fullStr |
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin |
title_full_unstemmed |
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin |
title_sort |
non-collagenous dentin protein binding sites control mineral formation during the biomineralisation process in radicular dentin |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-02-01 |
description |
The biomineralisation of radicular dentin involves complex molecular signalling. Providing evidence of protein binding sites for calcium ions and mineral precipitation is essential for a better understanding of the remineralisation process. This study aimed to evaluate the functional relationship of metalloproteinases (MMPs) and non-collagenous proteins (NCPs) with mineral initiation and maturation during the biomineralisation of radicular dentin. A standardized demineralisation procedure was performed to radicular dentin slices. Samples were remineralised in a PBS-bioactive material system for different periods of time. Assessments of ion exchange, Raman analysis, and energy dispersive X-ray analysis (EDAX) with a scanning electron microscope (SEM) were used to evaluate the remineralisation process. Immunohistochemistry and zymography were performed to analyse NCPs and MMPs expression. SEM evaluation showed that the mineral nucleation and growth occurs, exclusively, on the demineralised radicular dentin surface. Raman analysis of remineralised dentin showed intense peaks at 955 and 1063 cm<sup>−1</sup>, which can be attributed to carbonate apatite formation. Immunohistochemistry of demineralised samples revealed the presence of DMP1-CT, mainly in intratubular dentin, whereas DSPP in intratubular and intertubular dentin. DMP1-CT and DSPP binding sites control carbonate apatite nucleation and maturation guiding the remineralisation of radicular dentin. |
topic |
biomineralisation carbonate apatite bioactive materials dentin remineralization matrix metalloproteinases non-collagenous proteins radicular dentin |
url |
https://www.mdpi.com/1996-1944/13/5/1053 |
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