The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma
Background: the neoplastic B cells of the <i>Helicobacter pylori</i>-related low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma proliferate in response to <i>H. pylori</i>, however, the nature of the <i>H. pylori</i> antigen responsible for prolif...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-08-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/17/9459 |
id |
doaj-e1ac3d7d138043d4a3e99b9c304ae04b |
---|---|
record_format |
Article |
spelling |
doaj-e1ac3d7d138043d4a3e99b9c304ae04b2021-09-09T13:48:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01229459945910.3390/ijms22179459The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT LymphomaChiara Della Bella0Maria Felicia Soluri1Simone Puccio2Marisa Benagiano3Alessia Grassi4Jacopo Bitetti5Fabio Cianchi6Daniele Sblattero7Clelia Peano8Mario Milco D’Elios9Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Health Sciences & IRCAD, Università del Piemonte Orientale, 28100 Novara, ItalyGenomic Unit, IRCCS, Humanitas Clinical and Research Center, 20090 Milan, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyInstitute of Genetic and Biomedical Research, UoS Milan, National Research Council, 20090 Milan, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyBackground: the neoplastic B cells of the <i>Helicobacter pylori</i>-related low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma proliferate in response to <i>H. pylori</i>, however, the nature of the <i>H. pylori</i> antigen responsible for proliferation is still unknown. The purpose of the study was to dissect whether CagY might be the <i>H. pylori</i> antigen able to drive B cell proliferation. Methods: the B cells and the clonal progeny of T cells from the gastric mucosa of five patients with MALT lymphoma were compared with those of T cell clones obtained from five <i>H. pylori</i>–infected patients with chronic gastritis. The T cell clones were assessed for their specificity to <i>H. pylori</i> CagY, cytokine profile and helper function for B cell proliferation. Results: 22 of 158 CD4<sup>+</sup> (13.9%) gastric clones from MALT lymphoma and three of 179 CD4<sup>+</sup> (1.7%) clones from chronic gastritis recognized CagY. CagY predominantly drives Interferon-gamma (IFN-γ) and Interleukin-17 (IL-17) secretion by gastric CD4<sup>+</sup> T cells from <i>H. pylori</i>-infected patients with low-grade gastric MALT lymphoma. All MALT lymphoma-derived clones dose dependently increased their B cell help, whereas clones from chronic gastritis lost helper activity at T-to-B-cell ratios greater than 1. Conclusion: the results obtained indicate that CagY drives both B cell proliferation and T cell activation in gastric MALT lymphomas.https://www.mdpi.com/1422-0067/22/17/9459<i>Helicobacter pylori</i>CagYB cellsT cellscytokinesMALT |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chiara Della Bella Maria Felicia Soluri Simone Puccio Marisa Benagiano Alessia Grassi Jacopo Bitetti Fabio Cianchi Daniele Sblattero Clelia Peano Mario Milco D’Elios |
spellingShingle |
Chiara Della Bella Maria Felicia Soluri Simone Puccio Marisa Benagiano Alessia Grassi Jacopo Bitetti Fabio Cianchi Daniele Sblattero Clelia Peano Mario Milco D’Elios The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma International Journal of Molecular Sciences <i>Helicobacter pylori</i> CagY B cells T cells cytokines MALT |
author_facet |
Chiara Della Bella Maria Felicia Soluri Simone Puccio Marisa Benagiano Alessia Grassi Jacopo Bitetti Fabio Cianchi Daniele Sblattero Clelia Peano Mario Milco D’Elios |
author_sort |
Chiara Della Bella |
title |
The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma |
title_short |
The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma |
title_full |
The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma |
title_fullStr |
The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma |
title_full_unstemmed |
The <i>Helicobacter pylori</i> CagY Protein Drives Gastric Th1 and Th17 Inflammation and B Cell Proliferation in Gastric MALT Lymphoma |
title_sort |
<i>helicobacter pylori</i> cagy protein drives gastric th1 and th17 inflammation and b cell proliferation in gastric malt lymphoma |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
Background: the neoplastic B cells of the <i>Helicobacter pylori</i>-related low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma proliferate in response to <i>H. pylori</i>, however, the nature of the <i>H. pylori</i> antigen responsible for proliferation is still unknown. The purpose of the study was to dissect whether CagY might be the <i>H. pylori</i> antigen able to drive B cell proliferation. Methods: the B cells and the clonal progeny of T cells from the gastric mucosa of five patients with MALT lymphoma were compared with those of T cell clones obtained from five <i>H. pylori</i>–infected patients with chronic gastritis. The T cell clones were assessed for their specificity to <i>H. pylori</i> CagY, cytokine profile and helper function for B cell proliferation. Results: 22 of 158 CD4<sup>+</sup> (13.9%) gastric clones from MALT lymphoma and three of 179 CD4<sup>+</sup> (1.7%) clones from chronic gastritis recognized CagY. CagY predominantly drives Interferon-gamma (IFN-γ) and Interleukin-17 (IL-17) secretion by gastric CD4<sup>+</sup> T cells from <i>H. pylori</i>-infected patients with low-grade gastric MALT lymphoma. All MALT lymphoma-derived clones dose dependently increased their B cell help, whereas clones from chronic gastritis lost helper activity at T-to-B-cell ratios greater than 1. Conclusion: the results obtained indicate that CagY drives both B cell proliferation and T cell activation in gastric MALT lymphomas. |
topic |
<i>Helicobacter pylori</i> CagY B cells T cells cytokines MALT |
url |
https://www.mdpi.com/1422-0067/22/17/9459 |
work_keys_str_mv |
AT chiaradellabella theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT mariafeliciasoluri theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT simonepuccio theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT marisabenagiano theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT alessiagrassi theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT jacopobitetti theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT fabiocianchi theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT danielesblattero theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT cleliapeano theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT mariomilcodelios theihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT chiaradellabella ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT mariafeliciasoluri ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT simonepuccio ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT marisabenagiano ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT alessiagrassi ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT jacopobitetti ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT fabiocianchi ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT danielesblattero ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT cleliapeano ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma AT mariomilcodelios ihelicobacterpyloriicagyproteindrivesgastricth1andth17inflammationandbcellproliferationingastricmaltlymphoma |
_version_ |
1717760127245746176 |