Quantitative analysis of RNAscope staining for c-fos expression in mouse brain tissue as a measure of Neuronal Activation

Quantitative analysis of RNAscope staining for c-fos expression in mouse brain tissue as a measure of Neuronal Activation

The expression of c-fos mRNA is an indirect marker of neuronal activity. RNAscope ACD Bio RNAscope (now Biotechne) is a proprietary in-situ mRNA detection technology using branched DNA amplification and z paired probes to deliver a robust and specific assay designed primarily for use on formalin fix...

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Bibliographic Details
Main Authors: Cristina Cosi, Michael Millar, Mariana Beltran, Lorcan Sherry, Silvia Gatti-McArthur
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
Brain c-fos RNAscope in-situ hybridisation
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121001412

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