Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L.
Abatract: Miniature inverted-repeat transposable elements (MITEs) are a group of DNA transposable element (TE) which preferentially distributed with gene associated regions. Tens of MITEs families have been revealed in Brassica napus genome, they scatter across the genome with tens of thousands copi...
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KeAi Communications Co., Ltd.
2021-03-01
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doaj-e1bf72e9e33546fa840e838ea6e7a1392021-03-29T04:10:33ZengKeAi Communications Co., Ltd.Oil Crop Science2096-24282021-03-01612834Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L.Shutao Dai0Jinna Hou1Maomao Qin2Ziju Dai3Xiaojie Jin4Shilei Zhao5Yanhua Dong6Yongxia Wang7Zhengqing Wu8Zhensheng Lei9Institute of Cereal Crops, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaSanmenxia Academy of Agricultural Sciences, Sanmenxia, ChinaHenan Agricultural Science and Technology Exhibition Hall, Zhengzhou, ChinaInstitute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, China; Corresponding author.Institute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, China; Corresponding author.Abatract: Miniature inverted-repeat transposable elements (MITEs) are a group of DNA transposable element (TE) which preferentially distributed with gene associated regions. Tens of MITEs families have been revealed in Brassica napus genome, they scatter across the genome with tens of thousands copies and produce polymorphisms both intra- and inter-species. Our previous studies revealed a Tourist-like MITE, Monkey King, associated with vernalization requirement of B. napus, however there are still few studies reveal MITE association with agricultural traits in B. napus.In the present study, 80 polymorphic markers were developed from 55 MITEs, and used to evaluate genetic diversity in a panel of B. napus accessions consisting of 101 natural and 25 synthetic genotypes. Five agricultural traits, oil content, glucosinolate content, erucic acid content, weight of thousand seeds (WTS) and plant height, were investigated across 3-years field experiments, in addition, two traits, hypocotyl length and root length, were evaluated at the 4-leaf stage in the laboratory. Correlations between the MITE-based markers and seven traits were analyzed, finally, 10 polymorphic markers produced by 6 pairs of MITE specific primers were revealed relatively high correlation with 5 traits. Two polymorphic markers were anchored with two candidate genes, BnaA02g13530D and BnaA08g20010D, respectively, which may contribute to glucosinolate content and WTS. This research may contribute to genetic improvement through utilization of MITE-induced polymorphisms in Brassica species.http://www.sciencedirect.com/science/article/pii/S2096242821000178Brassica napus L.Miniature inverted-repeat transposable elementsOil contentGlucosinolate contentErucic acid content1000-Seed weight |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shutao Dai Jinna Hou Maomao Qin Ziju Dai Xiaojie Jin Shilei Zhao Yanhua Dong Yongxia Wang Zhengqing Wu Zhensheng Lei |
spellingShingle |
Shutao Dai Jinna Hou Maomao Qin Ziju Dai Xiaojie Jin Shilei Zhao Yanhua Dong Yongxia Wang Zhengqing Wu Zhensheng Lei Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L. Oil Crop Science Brassica napus L. Miniature inverted-repeat transposable elements Oil content Glucosinolate content Erucic acid content 1000-Seed weight |
author_facet |
Shutao Dai Jinna Hou Maomao Qin Ziju Dai Xiaojie Jin Shilei Zhao Yanhua Dong Yongxia Wang Zhengqing Wu Zhensheng Lei |
author_sort |
Shutao Dai |
title |
Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L. |
title_short |
Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L. |
title_full |
Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L. |
title_fullStr |
Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L. |
title_full_unstemmed |
Diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in Brassica napus L. |
title_sort |
diversity and association analysis of important agricultural trait based on miniature inverted-repeat transposable element specific marker in brassica napus l. |
publisher |
KeAi Communications Co., Ltd. |
series |
Oil Crop Science |
issn |
2096-2428 |
publishDate |
2021-03-01 |
description |
Abatract: Miniature inverted-repeat transposable elements (MITEs) are a group of DNA transposable element (TE) which preferentially distributed with gene associated regions. Tens of MITEs families have been revealed in Brassica napus genome, they scatter across the genome with tens of thousands copies and produce polymorphisms both intra- and inter-species. Our previous studies revealed a Tourist-like MITE, Monkey King, associated with vernalization requirement of B. napus, however there are still few studies reveal MITE association with agricultural traits in B. napus.In the present study, 80 polymorphic markers were developed from 55 MITEs, and used to evaluate genetic diversity in a panel of B. napus accessions consisting of 101 natural and 25 synthetic genotypes. Five agricultural traits, oil content, glucosinolate content, erucic acid content, weight of thousand seeds (WTS) and plant height, were investigated across 3-years field experiments, in addition, two traits, hypocotyl length and root length, were evaluated at the 4-leaf stage in the laboratory. Correlations between the MITE-based markers and seven traits were analyzed, finally, 10 polymorphic markers produced by 6 pairs of MITE specific primers were revealed relatively high correlation with 5 traits. Two polymorphic markers were anchored with two candidate genes, BnaA02g13530D and BnaA08g20010D, respectively, which may contribute to glucosinolate content and WTS. This research may contribute to genetic improvement through utilization of MITE-induced polymorphisms in Brassica species. |
topic |
Brassica napus L. Miniature inverted-repeat transposable elements Oil content Glucosinolate content Erucic acid content 1000-Seed weight |
url |
http://www.sciencedirect.com/science/article/pii/S2096242821000178 |
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