Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis
Periodontitis is a highly prevalent chronic inflammatory disease leading to periodontal tissue breakdown and subsequent tooth loss, in which excessive host immune response accounts for most of the tissue damage and disease progression. Despite of the imperative need to develop host modulation therap...
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Frontiers Media S.A.
2021-09-01
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Series: | Frontiers in Immunology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.711337/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shu-jiao Qian Shu-jiao Qian Shu-jiao Qian Qian-ru Huang Rui-ying Chen Rui-ying Chen Rui-ying Chen Jia-ji Mo Jia-ji Mo Jia-ji Mo Lin-yi Zhou Lin-yi Zhou Lin-yi Zhou Yi Zhao Bin Li Bin Li Hong-chang Lai Hong-chang Lai Hong-chang Lai |
spellingShingle |
Shu-jiao Qian Shu-jiao Qian Shu-jiao Qian Qian-ru Huang Rui-ying Chen Rui-ying Chen Rui-ying Chen Jia-ji Mo Jia-ji Mo Jia-ji Mo Lin-yi Zhou Lin-yi Zhou Lin-yi Zhou Yi Zhao Bin Li Bin Li Hong-chang Lai Hong-chang Lai Hong-chang Lai Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis Frontiers in Immunology single-cell sequencing gingiva periodontitis inflammatory microenvironment mucosa |
author_facet |
Shu-jiao Qian Shu-jiao Qian Shu-jiao Qian Qian-ru Huang Rui-ying Chen Rui-ying Chen Rui-ying Chen Jia-ji Mo Jia-ji Mo Jia-ji Mo Lin-yi Zhou Lin-yi Zhou Lin-yi Zhou Yi Zhao Bin Li Bin Li Hong-chang Lai Hong-chang Lai Hong-chang Lai |
author_sort |
Shu-jiao Qian |
title |
Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis |
title_short |
Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis |
title_full |
Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis |
title_fullStr |
Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis |
title_full_unstemmed |
Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis |
title_sort |
single-cell rna sequencing identifies new inflammation-promoting cell subsets in asian patients with chronic periodontitis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-09-01 |
description |
Periodontitis is a highly prevalent chronic inflammatory disease leading to periodontal tissue breakdown and subsequent tooth loss, in which excessive host immune response accounts for most of the tissue damage and disease progression. Despite of the imperative need to develop host modulation therapy, the inflammatory responses and cell population dynamics which are finely tuned by the pathological microenvironment in periodontitis remained unclear. To investigate the local microenvironment of the inflammatory response in periodontitis, 10 periodontitis patients and 10 healthy volunteers were involved in this study. Single-cell transcriptomic profilings of gingival tissues from two patients and two healthy donors were performed. Histology, immunohistochemistry, and flow cytometry analysis were performed to further validate the identified cell subtypes and their involvement in periodontitis. Based on our single-cell resolution analysis, we identified HLA-DR-expressing endothelial cells and CXCL13+ fibroblasts which are highly associated with immune regulation. We also revealed the involvement of the proinflammatory NLRP3+ macrophages in periodontitis. We further showed the increased cell-cell communication between macrophage and T/B cells in the inflammatory periodontal tissues. Our data generated an intriguing catalog of cell types and interaction networks in the human gingiva and identified new inflammation-promoting cell subtypes involved in chronic periodontitis, which will be helpful in advancing host modulation therapy. |
topic |
single-cell sequencing gingiva periodontitis inflammatory microenvironment mucosa |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.711337/full |
work_keys_str_mv |
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1717762855192756225 |
spelling |
doaj-e1cb9c4d415f46b2acb4cc2ddb7d51972021-09-08T04:35:38ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-09-011210.3389/fimmu.2021.711337711337Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic PeriodontitisShu-jiao Qian0Shu-jiao Qian1Shu-jiao Qian2Qian-ru Huang3Rui-ying Chen4Rui-ying Chen5Rui-ying Chen6Jia-ji Mo7Jia-ji Mo8Jia-ji Mo9Lin-yi Zhou10Lin-yi Zhou11Lin-yi Zhou12Yi Zhao13Bin Li14Bin Li15Hong-chang Lai16Hong-chang Lai17Hong-chang Lai18Department of Oral and Maxillo-facial Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Oral and Maxillo-facial Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Oral and Maxillo-facial Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Oral and Maxillo-facial Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Immunology and Microbiology, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Oral and Maxillo-facial Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, ChinaPeriodontitis is a highly prevalent chronic inflammatory disease leading to periodontal tissue breakdown and subsequent tooth loss, in which excessive host immune response accounts for most of the tissue damage and disease progression. Despite of the imperative need to develop host modulation therapy, the inflammatory responses and cell population dynamics which are finely tuned by the pathological microenvironment in periodontitis remained unclear. To investigate the local microenvironment of the inflammatory response in periodontitis, 10 periodontitis patients and 10 healthy volunteers were involved in this study. Single-cell transcriptomic profilings of gingival tissues from two patients and two healthy donors were performed. Histology, immunohistochemistry, and flow cytometry analysis were performed to further validate the identified cell subtypes and their involvement in periodontitis. Based on our single-cell resolution analysis, we identified HLA-DR-expressing endothelial cells and CXCL13+ fibroblasts which are highly associated with immune regulation. We also revealed the involvement of the proinflammatory NLRP3+ macrophages in periodontitis. We further showed the increased cell-cell communication between macrophage and T/B cells in the inflammatory periodontal tissues. Our data generated an intriguing catalog of cell types and interaction networks in the human gingiva and identified new inflammation-promoting cell subtypes involved in chronic periodontitis, which will be helpful in advancing host modulation therapy.https://www.frontiersin.org/articles/10.3389/fimmu.2021.711337/fullsingle-cell sequencinggingivaperiodontitisinflammatory microenvironmentmucosa |