Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection.
B cells are typically characterized as positive regulators of the immune response, primarily by producing antibodies. However, recent studies indicate that various subsets of B cells can perform regulatory functions mainly through IL-10 secretion. Here we discovered that peritoneal B-1 (B-1P) cells...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-07-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2897882?pdf=render |
id |
doaj-e1d9d7335a0a486bb409b84335d05a08 |
---|---|
record_format |
Article |
spelling |
doaj-e1d9d7335a0a486bb409b84335d05a082020-11-25T01:55:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-07-0157e1144510.1371/journal.pone.0011445Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection.Vishal SindhavaMichael E WoodmanBrian StevensonSubbarao BondadaB cells are typically characterized as positive regulators of the immune response, primarily by producing antibodies. However, recent studies indicate that various subsets of B cells can perform regulatory functions mainly through IL-10 secretion. Here we discovered that peritoneal B-1 (B-1P) cells produce high levels of IL-10 upon stimulation with several Toll-like receptor (TLR) ligands. High levels of IL-10 suppressed B-1P cell proliferation and differentiation response to all TLR ligands studied in an autocrine manner in vitro and in vivo. IL-10 that accumulated in cultures inhibited B-1P cells at second and subsequent cell divisions mainly at the G1/S interphase. IL-10 inhibits TLR induced B-1P cell activation by blocking the classical NF-kappaB pathway. Co-stimulation with CD40 or BAFF abrogated the IL-10 inhibitory effect on B-1P cells during TLR stimulation. Finally, B-1P cells adoptively transferred from the peritoneal cavity of IL-10(-/-) mice showed better clearance of Borrelia hermsii than wild-type B-1P cells. This study described a novel autoregulatory property of B-1P cells mediated by B-1P cell derived IL-10, which may affect the function of B-1P cells in infection and autoimmunity.http://europepmc.org/articles/PMC2897882?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vishal Sindhava Michael E Woodman Brian Stevenson Subbarao Bondada |
spellingShingle |
Vishal Sindhava Michael E Woodman Brian Stevenson Subbarao Bondada Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection. PLoS ONE |
author_facet |
Vishal Sindhava Michael E Woodman Brian Stevenson Subbarao Bondada |
author_sort |
Vishal Sindhava |
title |
Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection. |
title_short |
Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection. |
title_full |
Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection. |
title_fullStr |
Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection. |
title_full_unstemmed |
Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection. |
title_sort |
interleukin-10 mediated autoregulation of murine b-1 b-cells and its role in borrelia hermsii infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-07-01 |
description |
B cells are typically characterized as positive regulators of the immune response, primarily by producing antibodies. However, recent studies indicate that various subsets of B cells can perform regulatory functions mainly through IL-10 secretion. Here we discovered that peritoneal B-1 (B-1P) cells produce high levels of IL-10 upon stimulation with several Toll-like receptor (TLR) ligands. High levels of IL-10 suppressed B-1P cell proliferation and differentiation response to all TLR ligands studied in an autocrine manner in vitro and in vivo. IL-10 that accumulated in cultures inhibited B-1P cells at second and subsequent cell divisions mainly at the G1/S interphase. IL-10 inhibits TLR induced B-1P cell activation by blocking the classical NF-kappaB pathway. Co-stimulation with CD40 or BAFF abrogated the IL-10 inhibitory effect on B-1P cells during TLR stimulation. Finally, B-1P cells adoptively transferred from the peritoneal cavity of IL-10(-/-) mice showed better clearance of Borrelia hermsii than wild-type B-1P cells. This study described a novel autoregulatory property of B-1P cells mediated by B-1P cell derived IL-10, which may affect the function of B-1P cells in infection and autoimmunity. |
url |
http://europepmc.org/articles/PMC2897882?pdf=render |
work_keys_str_mv |
AT vishalsindhava interleukin10mediatedautoregulationofmurineb1bcellsanditsroleinborreliahermsiiinfection AT michaelewoodman interleukin10mediatedautoregulationofmurineb1bcellsanditsroleinborreliahermsiiinfection AT brianstevenson interleukin10mediatedautoregulationofmurineb1bcellsanditsroleinborreliahermsiiinfection AT subbaraobondada interleukin10mediatedautoregulationofmurineb1bcellsanditsroleinborreliahermsiiinfection |
_version_ |
1724982719752962048 |