Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray.
We analyzed the urine samples of patients with type 2 diabetes at various stages of diabetic nephropathy by lectin microarray to identify a biomarker to predict the progression of diabetic nephropathy. Japanese patients with type 2 diabetes at various stages of nephropathy were enrolled and we perfo...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3797112?pdf=render |
id |
doaj-e1e5458e6c9e46c1b7862d7317f16ddb |
---|---|
record_format |
Article |
spelling |
doaj-e1e5458e6c9e46c1b7862d7317f16ddb2020-11-24T21:54:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7711810.1371/journal.pone.0077118Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray.Kentaro InoueJun WadaJun EguchiAtsuko NakatsukaSanae TeshigawaraKazutoshi MurakamiDaisuke OgawaTakahiro TeramiAkihiro KatayamaAtsuhito ToneIzumi IsedaKazuyuki HidaMasao YamadaTomohisa OgawaHirofumi MakinoWe analyzed the urine samples of patients with type 2 diabetes at various stages of diabetic nephropathy by lectin microarray to identify a biomarker to predict the progression of diabetic nephropathy. Japanese patients with type 2 diabetes at various stages of nephropathy were enrolled and we performed lectin microarray analyses (n = 17) and measured urinary excretion of fetuin-A (n = 85). The increased signals of urine samples were observed in Siaα2-6Gal/GalNAc-binding lectins (SNA, SSA, TJA-I) during the progression of diabetic nephropathy. We next isolated sialylated glycoproteins by using SSA-lectin affinity chromatography and identified fetuin-A by liquid chromatography-tandem mass spectrometer. Urinary excretion of fetuin-A significantly increased during the progression of albuminuria (A1, 0.40 ± 0.43; A2, 0.60 ± 0.53; A3 1.57 ± 1.13 ng/gCr; p = 7.29 × 10(-8)) and of GFR stages (G1, 0.39 ± 0.39; G2, 0.49 ± 0.45; G3, 1.25 ± 1.18; G4, 1.34 ± 0.80 ng/gCr; p = 3.89 × 10(-4)). Multivariate logistic regression analysis was employed to assess fetuin-A as a risk for diabetic nephropathy with microalbuminuria or GFR<60 mL/min. Fetuin-A is demonstrated as a risk factor for both microalbuminuria and reduction of GFR in diabetic nephropathy with the odds ratio of 4.721 (1.881-11.844) and 3.739 (1.785-7.841), respectively. Collectively, the glycan profiling analysis is useful method to identify the urine biomarkers and fetuin-A is a candidate to predict the progression of diabetic nephropathy.http://europepmc.org/articles/PMC3797112?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kentaro Inoue Jun Wada Jun Eguchi Atsuko Nakatsuka Sanae Teshigawara Kazutoshi Murakami Daisuke Ogawa Takahiro Terami Akihiro Katayama Atsuhito Tone Izumi Iseda Kazuyuki Hida Masao Yamada Tomohisa Ogawa Hirofumi Makino |
spellingShingle |
Kentaro Inoue Jun Wada Jun Eguchi Atsuko Nakatsuka Sanae Teshigawara Kazutoshi Murakami Daisuke Ogawa Takahiro Terami Akihiro Katayama Atsuhito Tone Izumi Iseda Kazuyuki Hida Masao Yamada Tomohisa Ogawa Hirofumi Makino Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. PLoS ONE |
author_facet |
Kentaro Inoue Jun Wada Jun Eguchi Atsuko Nakatsuka Sanae Teshigawara Kazutoshi Murakami Daisuke Ogawa Takahiro Terami Akihiro Katayama Atsuhito Tone Izumi Iseda Kazuyuki Hida Masao Yamada Tomohisa Ogawa Hirofumi Makino |
author_sort |
Kentaro Inoue |
title |
Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. |
title_short |
Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. |
title_full |
Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. |
title_fullStr |
Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. |
title_full_unstemmed |
Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. |
title_sort |
urinary fetuin-a is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
We analyzed the urine samples of patients with type 2 diabetes at various stages of diabetic nephropathy by lectin microarray to identify a biomarker to predict the progression of diabetic nephropathy. Japanese patients with type 2 diabetes at various stages of nephropathy were enrolled and we performed lectin microarray analyses (n = 17) and measured urinary excretion of fetuin-A (n = 85). The increased signals of urine samples were observed in Siaα2-6Gal/GalNAc-binding lectins (SNA, SSA, TJA-I) during the progression of diabetic nephropathy. We next isolated sialylated glycoproteins by using SSA-lectin affinity chromatography and identified fetuin-A by liquid chromatography-tandem mass spectrometer. Urinary excretion of fetuin-A significantly increased during the progression of albuminuria (A1, 0.40 ± 0.43; A2, 0.60 ± 0.53; A3 1.57 ± 1.13 ng/gCr; p = 7.29 × 10(-8)) and of GFR stages (G1, 0.39 ± 0.39; G2, 0.49 ± 0.45; G3, 1.25 ± 1.18; G4, 1.34 ± 0.80 ng/gCr; p = 3.89 × 10(-4)). Multivariate logistic regression analysis was employed to assess fetuin-A as a risk for diabetic nephropathy with microalbuminuria or GFR<60 mL/min. Fetuin-A is demonstrated as a risk factor for both microalbuminuria and reduction of GFR in diabetic nephropathy with the odds ratio of 4.721 (1.881-11.844) and 3.739 (1.785-7.841), respectively. Collectively, the glycan profiling analysis is useful method to identify the urine biomarkers and fetuin-A is a candidate to predict the progression of diabetic nephropathy. |
url |
http://europepmc.org/articles/PMC3797112?pdf=render |
work_keys_str_mv |
AT kentaroinoue urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT junwada urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT juneguchi urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT atsukonakatsuka urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT sanaeteshigawara urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT kazutoshimurakami urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT daisukeogawa urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT takahiroterami urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT akihirokatayama urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT atsuhitotone urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT izumiiseda urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT kazuyukihida urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT masaoyamada urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT tomohisaogawa urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray AT hirofumimakino urinaryfetuinaisanovelmarkerfordiabeticnephropathyintype2diabetesidentifiedbylectinmicroarray |
_version_ |
1725866362578927616 |