Effect of kekeling mixture on the levels of IL-6 and IL-8 in RSV infected mice

• Main Authors: Xiao-Yun Wei, Na Wei
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2020-07-01
• Series: Journal of Hainan Medical University
• Subjects: kekeling mixture; rsv infection; il-6; il-8
• Online Access: http://www.hnykdxxb.com/PDF/202013/05.pdf

Objective: To explore the effect of kekeling mixture on the levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) in mice infected with respiratory syncytial virus (RSV), so as to provide theoretical basis for the treatment of RSA. Methods: 60 healthy male SD mice were randomly divided into six groups: healthy group, model group, low, medium, high dose group and ribavirin group, with 10 mice in each group. The general conditions of each group were observed and recorded after the establishment of RSV. Western blot (WB) and enzyme linked immunosorbent assay (ELISA) were used to detect the level of IL-6 and IL-8 in lung lavage fluid and lung tissue. Results: the RSV model was established successfully in the normal group because of the RSV infection. The alveolar epithelial cells in the normal group were relatively intact, the bronchi and pulmonary septum were intact, the alveolar epithelial cells in the RSV infected group were damaged, and the blood vessels were swollen and congested. The IL-6 and IL-8 water in the alveolar lavage fluid and lung tissue in the RSV infected group were higher than those in the normal group on average The difference was statistically significant (P < 0.05); compared with the model group, the level of IL-6 and IL-8 in BALF and lung tissue decreased significantly after treatment (P< 0.05), and the content of IL-6 and IL-8 in BALF and lung tissue in each group was significantly higher than that in ribavirin group (P< 0.05). Conclusion: kekeling can reduce the level of IL-6 and IL-8 in BALF and lung tissue of RVs infected mice to a certain extent. It is speculated that kekeling mixture can be used as a potential drug to prevent and treat RSV infection.