The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes
Abstract Neutral electrolyzed water (NEW) was tested as a disinfectant against Listeria monocytogenes on the surface of table eggs. Eggs were collected from a single Bovans White flock and were exposed to L. monocytogenes. Artificially contaminated eggs were divided into three different treatment gr...
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doaj-e1e842e77bdc4f19842f237033e4bdf62020-11-25T01:02:27ZengWileyFood Science & Nutrition2048-71772019-07-01772252226010.1002/fsn3.1053The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenesAndres Rivera‐Garcia0Liliana Santos‐Ferro1Juan C. Ramirez‐Orejel2Lourdes T. Agredano‐Moreno3Luis F. Jimenez‐Garcia4David Paez‐Esquiliano5Eduardo Andrade‐Esquivel6Jose A. Cano‐Buendia7Facultad de Medicina Veterinaria y Zootecnia, Department of Microbiology and Immunology Universidad Nacional Autónoma de Mexico (UNAM) Mexico City MexicoFacultad de Medicina Veterinaria y Zootecnia, Department of Microbiology and Immunology Universidad Nacional Autónoma de Mexico (UNAM) Mexico City MexicoFacultad de Medicina Veterinaria y Zootecnia, Department of Animal Nutrition and Biochemistry Universidad Nacional Autónoma de México (UNAM) Mexico City MexicoCell Nanobiology Laboratory, Department of Cell Biology. Faculty of Sciences Universidad Nacional Autónoma de México (UNAM) Mexico City MexicoCell Nanobiology Laboratory, Department of Cell Biology. Faculty of Sciences Universidad Nacional Autónoma de México (UNAM) Mexico City MexicoFacultad de Medicina Veterinaria y Zootecnia, Department of Physiology and Pharmacology Universidad Nacional Autónoma de México (UNAM) Mexico City MexicoDepartamento de Ingenieria Bioquímica Instituto Tecnologico de Celaya Guanajuato MexicoFacultad de Medicina Veterinaria y Zootecnia, Department of Microbiology and Immunology Universidad Nacional Autónoma de Mexico (UNAM) Mexico City MexicoAbstract Neutral electrolyzed water (NEW) was tested as a disinfectant against Listeria monocytogenes on the surface of table eggs. Eggs were collected from a single Bovans White flock and were exposed to L. monocytogenes. Artificially contaminated eggs were divided into three different treatment groups: NEW, 2% citric acid solution (CAS), and saline solution (SS). To evaluate the bactericidal effect, the Mexican norm for antimicrobial activity determination protocol was performed. The observed bactericidal effect was compared against those obtained from CAS and SS. Bacterial cells present on the eggshells were quantified. NEW exhibited a significantly higher bactericidal effect than CAS when evaluated on the surfaces of chicken eggshells (6.11 log10CFU/ml reduction in vitro and a 2.18 log10 CFU/egg reduction on eggs vs. 1.06 log10CFU/ml in vitro reduction and 1.74 log10CFU/egg). Additionally, CAS was found to react with the carbonate egg shield, resulting in a loss of cuticle integrity. Mineral content of NEW‐treated eggshells was similar to SS‐treated eggshells; however, CAS‐treated eggshells showed a significant decrease in phosphorous concentration compared to NEW treatment. In this study, we demonstrated the effect of NEW and CAS on the integrity of the L. monocytogenes wall using transmission electron microscopy. To the best of our knowledge, this is the first report of the effect of NEW against L. monocytogenes on eggshells. Our results show that NEW is a viable alternative solution for the disinfection of table eggs that does not affect the cuticle or shell.https://doi.org/10.1002/fsn3.1053egg disinfectionelectrolyzed waterListeria monocytogenes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andres Rivera‐Garcia Liliana Santos‐Ferro Juan C. Ramirez‐Orejel Lourdes T. Agredano‐Moreno Luis F. Jimenez‐Garcia David Paez‐Esquiliano Eduardo Andrade‐Esquivel Jose A. Cano‐Buendia |
spellingShingle |
Andres Rivera‐Garcia Liliana Santos‐Ferro Juan C. Ramirez‐Orejel Lourdes T. Agredano‐Moreno Luis F. Jimenez‐Garcia David Paez‐Esquiliano Eduardo Andrade‐Esquivel Jose A. Cano‐Buendia The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes Food Science & Nutrition egg disinfection electrolyzed water Listeria monocytogenes |
author_facet |
Andres Rivera‐Garcia Liliana Santos‐Ferro Juan C. Ramirez‐Orejel Lourdes T. Agredano‐Moreno Luis F. Jimenez‐Garcia David Paez‐Esquiliano Eduardo Andrade‐Esquivel Jose A. Cano‐Buendia |
author_sort |
Andres Rivera‐Garcia |
title |
The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes |
title_short |
The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes |
title_full |
The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes |
title_fullStr |
The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes |
title_full_unstemmed |
The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes |
title_sort |
effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with listeria monocytogenes |
publisher |
Wiley |
series |
Food Science & Nutrition |
issn |
2048-7177 |
publishDate |
2019-07-01 |
description |
Abstract Neutral electrolyzed water (NEW) was tested as a disinfectant against Listeria monocytogenes on the surface of table eggs. Eggs were collected from a single Bovans White flock and were exposed to L. monocytogenes. Artificially contaminated eggs were divided into three different treatment groups: NEW, 2% citric acid solution (CAS), and saline solution (SS). To evaluate the bactericidal effect, the Mexican norm for antimicrobial activity determination protocol was performed. The observed bactericidal effect was compared against those obtained from CAS and SS. Bacterial cells present on the eggshells were quantified. NEW exhibited a significantly higher bactericidal effect than CAS when evaluated on the surfaces of chicken eggshells (6.11 log10CFU/ml reduction in vitro and a 2.18 log10 CFU/egg reduction on eggs vs. 1.06 log10CFU/ml in vitro reduction and 1.74 log10CFU/egg). Additionally, CAS was found to react with the carbonate egg shield, resulting in a loss of cuticle integrity. Mineral content of NEW‐treated eggshells was similar to SS‐treated eggshells; however, CAS‐treated eggshells showed a significant decrease in phosphorous concentration compared to NEW treatment. In this study, we demonstrated the effect of NEW and CAS on the integrity of the L. monocytogenes wall using transmission electron microscopy. To the best of our knowledge, this is the first report of the effect of NEW against L. monocytogenes on eggshells. Our results show that NEW is a viable alternative solution for the disinfection of table eggs that does not affect the cuticle or shell. |
topic |
egg disinfection electrolyzed water Listeria monocytogenes |
url |
https://doi.org/10.1002/fsn3.1053 |
work_keys_str_mv |
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