Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells
Objective: Cellular damage induced by oxidative stress has been involved in the development of neurodegeneration. Curcumin, a dietary polyphenol found in the rhizome of Curcuma longa, has been shown, both in vitro and in vivo, to be an effective reactive oxygen scavenging molecule. We investigated...
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doaj-e23a3a1617394c888c41fae9c4d25ca02021-08-13T09:48:53ZengMahidol UniversitySiriraj Medical Journal2228-80822018-06-01702Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH CellsRungtip Soi-ampornkul0Sandeepa Ghimire1Wipawan Thangnipon2Nirut Suwanna3Chinnavuth Vatanashevanopakorn4Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 Objective: Cellular damage induced by oxidative stress has been involved in the development of neurodegeneration. Curcumin, a dietary polyphenol found in the rhizome of Curcuma longa, has been shown, both in vitro and in vivo, to be an effective reactive oxygen scavenging molecule. We investigated an anti-oxidative effect of curcumin against .H2O2-induced toxicity in human neuroblastoma cell line SK-N-SH. Methods: The SK-N-SH cells were pre-treated with curcumin 2 hours prior to H2O2 treatment. We measured cell viability, intracellular reactive oxygen species (ROS) levels, expression of apoptotic-related proteins and caspase-3 activity 24 hours post H2O2-induced cytoxicity. Results: Treatment with curcumin at concentrations ranging from 5 to 50 μg/mL was not cytotoxic. Pre-treatment with curcumin at the concentrations of 5 to 50 μg/mL prior to H2O2 exposure caused a significant decrease in intracellular ROS levels and a significant increase in cell viability in a dose-dependent manner. Expression of activated form of caspase-3 and BAX, a pro-apoptotic protein, measured by Western blotting were reduced when the SK-NSH cell line was pre-incubated with curcumin. The curcumin pre-treated cells also exhibited less caspase-3 activity. Conclusion: Curcumin has protective effects against H2O2-induced toxicity in a dose-dependent manner through its anti-apoptotic and anti-oxidative properties in an in vitro H2O2-treated SK-N-SH model. https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/129601Curcumin; apoptosis; anti-oxidant; oxidative stress; SK-N-SH cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rungtip Soi-ampornkul Sandeepa Ghimire Wipawan Thangnipon Nirut Suwanna Chinnavuth Vatanashevanopakorn |
spellingShingle |
Rungtip Soi-ampornkul Sandeepa Ghimire Wipawan Thangnipon Nirut Suwanna Chinnavuth Vatanashevanopakorn Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells Siriraj Medical Journal Curcumin; apoptosis; anti-oxidant; oxidative stress; SK-N-SH cells |
author_facet |
Rungtip Soi-ampornkul Sandeepa Ghimire Wipawan Thangnipon Nirut Suwanna Chinnavuth Vatanashevanopakorn |
author_sort |
Rungtip Soi-ampornkul |
title |
Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells |
title_short |
Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells |
title_full |
Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells |
title_fullStr |
Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells |
title_full_unstemmed |
Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells |
title_sort |
curcumin attenuates hydrogen peroxide-induced cytotoxicity in human neuroblastoma sk-n-sh cells |
publisher |
Mahidol University |
series |
Siriraj Medical Journal |
issn |
2228-8082 |
publishDate |
2018-06-01 |
description |
Objective: Cellular damage induced by oxidative stress has been involved in the development of neurodegeneration. Curcumin, a dietary polyphenol found in the rhizome of Curcuma longa, has been shown, both in vitro and in vivo,
to be an effective reactive oxygen scavenging molecule. We investigated an anti-oxidative effect of curcumin against .H2O2-induced toxicity in human neuroblastoma cell line SK-N-SH.
Methods: The SK-N-SH cells were pre-treated with curcumin 2 hours prior to H2O2 treatment. We measured cell viability, intracellular reactive oxygen species (ROS) levels, expression of apoptotic-related proteins and caspase-3
activity 24 hours post H2O2-induced cytoxicity.
Results: Treatment with curcumin at concentrations ranging from 5 to 50 μg/mL was not cytotoxic. Pre-treatment with curcumin at the concentrations of 5 to 50 μg/mL prior to H2O2 exposure caused a significant decrease in
intracellular ROS levels and a significant increase in cell viability in a dose-dependent manner. Expression of activated form of caspase-3 and BAX, a pro-apoptotic protein, measured by Western blotting were reduced when the SK-NSH
cell line was pre-incubated with curcumin. The curcumin pre-treated cells also exhibited less caspase-3 activity.
Conclusion: Curcumin has protective effects against H2O2-induced toxicity in a dose-dependent manner through its anti-apoptotic and anti-oxidative properties in an in vitro H2O2-treated SK-N-SH model.
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topic |
Curcumin; apoptosis; anti-oxidant; oxidative stress; SK-N-SH cells |
url |
https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/129601 |
work_keys_str_mv |
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