Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy
Abstract Epilepsy is one of the most common neurological disorders in humans. Recently, long noncoding RNAs (lncRNAs) have been reported to be important players in neurological diseases. Herein, this study aimed to examine the effect of lncRNA GAS5 on the occurrence of epilepsy in rat and cell model...
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doaj-e23cf80121c24ef8b33b5f9256eeab012020-11-25T01:11:46ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502019-09-0135952753410.1002/kjm2.12102Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsyBao‐Guang Li0Wen‐Juan Wu1Hua‐Cheng Zheng2Hua‐Fang Yang3Yue‐Xian Zuo4Xiao‐Pu Cui5Department of Neurology Children's Hospital of Hebei Province Shijiazhuang Hebei ChinaDepartment of Neurology Children's Hospital of Hebei Province Shijiazhuang Hebei ChinaDepartment of Neurology Children's Hospital of Hebei Province Shijiazhuang Hebei ChinaDepartment of Neurology Children's Hospital of Hebei Province Shijiazhuang Hebei ChinaDepartment of Neurology Children's Hospital of Hebei Province Shijiazhuang Hebei ChinaDepartment of Neurology Children's Hospital of Hebei Province Shijiazhuang Hebei ChinaAbstract Epilepsy is one of the most common neurological disorders in humans. Recently, long noncoding RNAs (lncRNAs) have been reported to be important players in neurological diseases. Herein, this study aimed to examine the effect of lncRNA GAS5 on the occurrence of epilepsy in rat and cell models of epileptic seizure. The expression of lncRNA GAS5 was measured in the established rat and cell models. The binding sites between lncRNA GAS5 and miR‐135a‐5p, as well as those between miR‐135a‐5p and 3′ untranslated region of KCNQ3 were predicted by miRDB and Targetscan, separately, followed by verification using dual‐luciferase reporter gene assay. The expression of miR‐135a‐5p was measured in response to the overexpression of lncRNA GAS5. The mRNA and protein levels of KCNQ3 were examined in response to overexpression of miR‐135a‐5p. Next, the latency of epilepsy and frequency of epileptic seizures were assessed in rats injected with Lv‐shGAS5 and Lv‐miR‐135a‐5p in epileptic seizure model. In the rat and cell models, lncRNA GAS5 was highly expressed when epileptic seizure was induced. The expression of miR‐135a‐5p was decreased by overexpression of lncRNA GAS5. Meanwhile, the mRNA and protein levels of KCNQ3 were decreased in response to knockdown of miR‐135a‐5p. After the treatment of Lv‐shGAS5 and Lv‐miR‐135a‐5p, the average latent period of epilepsy was prolonged and the frequency of seizures was decreased. The key findings of the present study provide evidence emphasizing that lncRNA GAS5 functions as a competitive endogenous RNA of miR‐135a‐5p to increase expression of KCNQ3, and lncRNA GAS5 silencing inhibited the occurrence and progression of epilepsy.https://doi.org/10.1002/kjm2.12102competitive endogenous RNAepilepsyKCNQ3lncRNA GAS5microRNA‐135a‐5p |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bao‐Guang Li Wen‐Juan Wu Hua‐Cheng Zheng Hua‐Fang Yang Yue‐Xian Zuo Xiao‐Pu Cui |
spellingShingle |
Bao‐Guang Li Wen‐Juan Wu Hua‐Cheng Zheng Hua‐Fang Yang Yue‐Xian Zuo Xiao‐Pu Cui Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy Kaohsiung Journal of Medical Sciences competitive endogenous RNA epilepsy KCNQ3 lncRNA GAS5 microRNA‐135a‐5p |
author_facet |
Bao‐Guang Li Wen‐Juan Wu Hua‐Cheng Zheng Hua‐Fang Yang Yue‐Xian Zuo Xiao‐Pu Cui |
author_sort |
Bao‐Guang Li |
title |
Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy |
title_short |
Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy |
title_full |
Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy |
title_fullStr |
Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy |
title_full_unstemmed |
Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy |
title_sort |
long noncoding rna gas5 silencing inhibits the expression of kcnq3 by sponging mir‐135a‐5p to prevent the progression of epilepsy |
publisher |
Wiley |
series |
Kaohsiung Journal of Medical Sciences |
issn |
1607-551X 2410-8650 |
publishDate |
2019-09-01 |
description |
Abstract Epilepsy is one of the most common neurological disorders in humans. Recently, long noncoding RNAs (lncRNAs) have been reported to be important players in neurological diseases. Herein, this study aimed to examine the effect of lncRNA GAS5 on the occurrence of epilepsy in rat and cell models of epileptic seizure. The expression of lncRNA GAS5 was measured in the established rat and cell models. The binding sites between lncRNA GAS5 and miR‐135a‐5p, as well as those between miR‐135a‐5p and 3′ untranslated region of KCNQ3 were predicted by miRDB and Targetscan, separately, followed by verification using dual‐luciferase reporter gene assay. The expression of miR‐135a‐5p was measured in response to the overexpression of lncRNA GAS5. The mRNA and protein levels of KCNQ3 were examined in response to overexpression of miR‐135a‐5p. Next, the latency of epilepsy and frequency of epileptic seizures were assessed in rats injected with Lv‐shGAS5 and Lv‐miR‐135a‐5p in epileptic seizure model. In the rat and cell models, lncRNA GAS5 was highly expressed when epileptic seizure was induced. The expression of miR‐135a‐5p was decreased by overexpression of lncRNA GAS5. Meanwhile, the mRNA and protein levels of KCNQ3 were decreased in response to knockdown of miR‐135a‐5p. After the treatment of Lv‐shGAS5 and Lv‐miR‐135a‐5p, the average latent period of epilepsy was prolonged and the frequency of seizures was decreased. The key findings of the present study provide evidence emphasizing that lncRNA GAS5 functions as a competitive endogenous RNA of miR‐135a‐5p to increase expression of KCNQ3, and lncRNA GAS5 silencing inhibited the occurrence and progression of epilepsy. |
topic |
competitive endogenous RNA epilepsy KCNQ3 lncRNA GAS5 microRNA‐135a‐5p |
url |
https://doi.org/10.1002/kjm2.12102 |
work_keys_str_mv |
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