Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal
Spermatogonial stem cells (SSCs) are required for spermatogenesis. Earlier studies showed that glial cell line-derived neurotrophic factor (GDNF) was indispensable for SSC self-renewal by binding to the GFRA1/RET receptor. Mice with mutations in these molecules showed impaired spermatogenesis, which...
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doaj-e24f462d60644bcf946444b6ed4bb75d2020-11-25T00:04:59ZengElsevierStem Cell Reports2213-67112015-03-014348950210.1016/j.stemcr.2015.01.010Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-RenewalSeiji Takashima0Mito Kanatsu-Shinohara1Takashi Tanaka2Hiroko Morimoto3Kimiko Inoue4Narumi Ogonuki5Mayumi Jijiwa6Masahide Takahashi7Atsuo Ogura8Takashi Shinohara9Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanThe Institute for Physical and Chemical Research (RIKEN), Bioresource Center, Tsukuba 305-0074, JapanThe Institute for Physical and Chemical Research (RIKEN), Bioresource Center, Tsukuba 305-0074, JapanDepartment of Pathology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, JapanDepartment of Pathology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, JapanThe Institute for Physical and Chemical Research (RIKEN), Bioresource Center, Tsukuba 305-0074, JapanDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanSpermatogonial stem cells (SSCs) are required for spermatogenesis. Earlier studies showed that glial cell line-derived neurotrophic factor (GDNF) was indispensable for SSC self-renewal by binding to the GFRA1/RET receptor. Mice with mutations in these molecules showed impaired spermatogenesis, which was attributed to SSC depletion. Here we show that SSCs undergo GDNF-independent self-renewal. A small number of spermatogonia formed colonies when testis fragments from a Ret mutant mouse strain were transplanted into heterologous recipients. Moreover, fibroblast growth factor 2 (FGF2) supplementation enabled in vitro SSC expansion without GDNF. Although GDNF-mediated self-renewal signaling required both AKT and MAP2K1/2, the latter was dispensable in FGF2-mediated self-renewal. FGF2-depleted testes exhibited increased levels of GDNF and were enriched for SSCs, suggesting that the balance between FGF2 and GDNF levels influences SSC self-renewal in vivo. Our results show that SSCs exhibit at least two modes of self-renewal and suggest complexity of SSC regulation in vivo.http://www.sciencedirect.com/science/article/pii/S2213671115000314 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seiji Takashima Mito Kanatsu-Shinohara Takashi Tanaka Hiroko Morimoto Kimiko Inoue Narumi Ogonuki Mayumi Jijiwa Masahide Takahashi Atsuo Ogura Takashi Shinohara |
spellingShingle |
Seiji Takashima Mito Kanatsu-Shinohara Takashi Tanaka Hiroko Morimoto Kimiko Inoue Narumi Ogonuki Mayumi Jijiwa Masahide Takahashi Atsuo Ogura Takashi Shinohara Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal Stem Cell Reports |
author_facet |
Seiji Takashima Mito Kanatsu-Shinohara Takashi Tanaka Hiroko Morimoto Kimiko Inoue Narumi Ogonuki Mayumi Jijiwa Masahide Takahashi Atsuo Ogura Takashi Shinohara |
author_sort |
Seiji Takashima |
title |
Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal |
title_short |
Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal |
title_full |
Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal |
title_fullStr |
Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal |
title_full_unstemmed |
Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal |
title_sort |
functional differences between gdnf-dependent and fgf2-dependent mouse spermatogonial stem cell self-renewal |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2015-03-01 |
description |
Spermatogonial stem cells (SSCs) are required for spermatogenesis. Earlier studies showed that glial cell line-derived neurotrophic factor (GDNF) was indispensable for SSC self-renewal by binding to the GFRA1/RET receptor. Mice with mutations in these molecules showed impaired spermatogenesis, which was attributed to SSC depletion. Here we show that SSCs undergo GDNF-independent self-renewal. A small number of spermatogonia formed colonies when testis fragments from a Ret mutant mouse strain were transplanted into heterologous recipients. Moreover, fibroblast growth factor 2 (FGF2) supplementation enabled in vitro SSC expansion without GDNF. Although GDNF-mediated self-renewal signaling required both AKT and MAP2K1/2, the latter was dispensable in FGF2-mediated self-renewal. FGF2-depleted testes exhibited increased levels of GDNF and were enriched for SSCs, suggesting that the balance between FGF2 and GDNF levels influences SSC self-renewal in vivo. Our results show that SSCs exhibit at least two modes of self-renewal and suggest complexity of SSC regulation in vivo. |
url |
http://www.sciencedirect.com/science/article/pii/S2213671115000314 |
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