<b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201

• Main Authors: Ianna Wivianne Fernandes de Araújo, José Ariévilo Gurgel Rodrigues, Edfranck de Sousa Oliveira Vanderlei, Gabriela Almeida de Paula, Ticiana de Brito Lima, Norma Maria Barros Benevides
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2012-03-01
• Series: Acta Scientiarum: Technology
• Subjects: red alga; sulfated galactans; extraction methods; peritonitis; APTT test
• Online Access: http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201

<p class="aresumo">Biochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating <em>iota</em>-carrageenans (ι-CARs) from <em>Solieria filiformis</em> (Rhodophyta) by enzymatic extraction (EE), refined hot-water extraction (RHWE) and hot-water extraction (HWE), and test (EE, s.c.) their anti-inflammatory effects in the peritonitis model using the Lambda-carrageenan (700 µg cavity^-1, i.p.) as an inflammatory stimuli in rats. The activated partial thromboplastin time (APTT) was also evaluated in ι-CARs fractions, obtained by ion-exchange chromatography (DEAE-cellulose), using rabbit plasma and compared to heparin (193 IU mg^-1). The results showed that the ι-CARs (EE) (3 or 9 mg kg^-1) containing 89.92% total sugars, 29.02% sulfate and absence of contaminant proteins inhibited (p &lt; 0.05) the cellular infiltrate in the peritoneal cavity of the animals, but with 27 mg kg^-1 had no anti-inflammatory effect (p &gt; 0.05). Similar chromatography profiles were obtained among the methods; however, with fractions revealing different pattern on charge density by electrophoresis. Fractions had no virtually effects on APTT (1.16, 1.73 and 1.59 IU mg^-1 for EE, RHWE and HWE, respectively). Further investigations to better understanding the actions of <em>S. filiformis</em> ι-CARs (EE) in the inflammatory response are suggested.</p>