The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation
This study investigated the effects of root-restriction cultivation on the root architecture, endogenous strigolactone (SL) content, and SL-related genes expression in grapevine (<i>Vitis vinifera</i> L.). In addition, we clarified the effects of synthetic SL analog GR24 application on g...
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doaj-e278dd3bc3844ceda1633883c299eb142021-08-26T13:52:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01228799879910.3390/ijms22168799The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction CultivationYan Xu0Jiyuan Wang1Ruiqi Wang2Lei Wang3Caixi Zhang4Wenping Xu5Shiping Wang6Songtao Jiu7Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaThis study investigated the effects of root-restriction cultivation on the root architecture, endogenous strigolactone (SL) content, and SL-related genes expression in grapevine (<i>Vitis vinifera</i> L.). In addition, we clarified the effects of synthetic SL analog GR24 application on grapevine roots to explore the role of SLs in their development. The results showed that the root architecture changed significantly under root-restriction cultivation. At 40 days after transplantation (DAT), the contents of two types of SLs in roots under root restriction were both significantly lower than that in roots of the control. SL content was significantly positively correlated with the expression levels of <i>VvCCD8</i> and <i>VvD27</i>, indicating that they play vital roles in SLs synthesis. After GR24 treatment for 20 days, the root length was significantly shorter than in the control. A low concentration (0.1 μM) of GR24 significantly reduced the root diameter and increased the fine-root density, while a high concentration (10 μM) of GR24 significantly reduced the lateral root (LR) length and increased the LR density. Concomitantly, GR24 (0.1 μM) reduced endogenous SL content. After GR24 treatment for 5 days, the total content of two tested SLs was highly positively correlated with the expression levels of <i>VvDAD2</i>, whereas it was highly negatively correlated with <i>VvSMAXL4</i> at 20 days after GR24 treatment. This study helps to clarify the internal mechanism of root-restriction cultivation affecting the changes in grapevine root architecture, as well as further explore the important role of SLs in the growth of grapevine roots in response to root-restriction treatment.https://www.mdpi.com/1422-0067/22/16/8799grapevineroot restrictionstrigolactoneroot architecturegene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Xu Jiyuan Wang Ruiqi Wang Lei Wang Caixi Zhang Wenping Xu Shiping Wang Songtao Jiu |
spellingShingle |
Yan Xu Jiyuan Wang Ruiqi Wang Lei Wang Caixi Zhang Wenping Xu Shiping Wang Songtao Jiu The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation International Journal of Molecular Sciences grapevine root restriction strigolactone root architecture gene expression |
author_facet |
Yan Xu Jiyuan Wang Ruiqi Wang Lei Wang Caixi Zhang Wenping Xu Shiping Wang Songtao Jiu |
author_sort |
Yan Xu |
title |
The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation |
title_short |
The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation |
title_full |
The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation |
title_fullStr |
The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation |
title_full_unstemmed |
The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine (<i>Vitis vinifera</i> L.) in Response to Root-Restriction Cultivation |
title_sort |
role of strigolactones in the regulation of root system architecture in grapevine (<i>vitis vinifera</i> l.) in response to root-restriction cultivation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
This study investigated the effects of root-restriction cultivation on the root architecture, endogenous strigolactone (SL) content, and SL-related genes expression in grapevine (<i>Vitis vinifera</i> L.). In addition, we clarified the effects of synthetic SL analog GR24 application on grapevine roots to explore the role of SLs in their development. The results showed that the root architecture changed significantly under root-restriction cultivation. At 40 days after transplantation (DAT), the contents of two types of SLs in roots under root restriction were both significantly lower than that in roots of the control. SL content was significantly positively correlated with the expression levels of <i>VvCCD8</i> and <i>VvD27</i>, indicating that they play vital roles in SLs synthesis. After GR24 treatment for 20 days, the root length was significantly shorter than in the control. A low concentration (0.1 μM) of GR24 significantly reduced the root diameter and increased the fine-root density, while a high concentration (10 μM) of GR24 significantly reduced the lateral root (LR) length and increased the LR density. Concomitantly, GR24 (0.1 μM) reduced endogenous SL content. After GR24 treatment for 5 days, the total content of two tested SLs was highly positively correlated with the expression levels of <i>VvDAD2</i>, whereas it was highly negatively correlated with <i>VvSMAXL4</i> at 20 days after GR24 treatment. This study helps to clarify the internal mechanism of root-restriction cultivation affecting the changes in grapevine root architecture, as well as further explore the important role of SLs in the growth of grapevine roots in response to root-restriction treatment. |
topic |
grapevine root restriction strigolactone root architecture gene expression |
url |
https://www.mdpi.com/1422-0067/22/16/8799 |
work_keys_str_mv |
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