Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate
We cloned and analyzed the two genes of the 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene family from Huperzia serrate. The two transcripts coding HDR, named HsHDR1 and HsHDR2, were discovered in the transcriptome dataset of H. serrate and were cloned by reverse transcription-p...
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doaj-e296571e2f934b8ba7b2ceb81c1522502020-11-25T02:29:36ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432015-11-015658358910.1016/j.apsb.2015.09.002Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrateHaizhou Lv0Xin Zhang1Baosheng Liao2Wanjing Liu3Liu He4Jingyuan Song5Chao Sun6Hongmei Luo7Shilin Chen8Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, ChinaWe cloned and analyzed the two genes of the 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene family from Huperzia serrate. The two transcripts coding HDR, named HsHDR1 and HsHDR2, were discovered in the transcriptome dataset of H. serrate and were cloned by reverse transcription-polymerase chain reaction (RT-PCR). The physicochemical properties, protein domains, protein secondary structure, and 3D structure of the putative HsHDR1 and HsHDR2 proteins were analyzed. The full-length cDNA of the HsHDR1 gene contained 1431 bp encoding a putative protein with 476 amino acids, whereas the HsHDR2 gene contained 1428 bp encoding a putative protein of 475 amino acids. These two proteins contained the conserved domain of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (PF02401), but without the transmembrane region and signal peptide. The most abundant expression of HsHDR1 and HsHDR2 was detected in H. serrate roots, followed by the stems and leaves. Our results provide a foundation for exploring the function of HsHDR1 and HsHDR2 in terpenoid and sterol biosynthesis in Huperziaceae plants.http://www.sciencedirect.com/science/article/pii/S22113835150013431-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductaseHuperzia serrateTerpenoidGene cloneBioinformatics analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haizhou Lv Xin Zhang Baosheng Liao Wanjing Liu Liu He Jingyuan Song Chao Sun Hongmei Luo Shilin Chen |
spellingShingle |
Haizhou Lv Xin Zhang Baosheng Liao Wanjing Liu Liu He Jingyuan Song Chao Sun Hongmei Luo Shilin Chen Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate Acta Pharmaceutica Sinica B 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase Huperzia serrate Terpenoid Gene clone Bioinformatics analysis |
author_facet |
Haizhou Lv Xin Zhang Baosheng Liao Wanjing Liu Liu He Jingyuan Song Chao Sun Hongmei Luo Shilin Chen |
author_sort |
Haizhou Lv |
title |
Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate |
title_short |
Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate |
title_full |
Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate |
title_fullStr |
Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate |
title_full_unstemmed |
Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate |
title_sort |
cloning and analysis of 1-hydroxy-2-methyl-2-(e)-butenyl-4-diphosphate reductase genes hshdr1 and hshdr2 in huperzia serrate |
publisher |
Elsevier |
series |
Acta Pharmaceutica Sinica B |
issn |
2211-3835 2211-3843 |
publishDate |
2015-11-01 |
description |
We cloned and analyzed the two genes of the 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene family from Huperzia serrate. The two transcripts coding HDR, named HsHDR1 and HsHDR2, were discovered in the transcriptome dataset of H. serrate and were cloned by reverse transcription-polymerase chain reaction (RT-PCR). The physicochemical properties, protein domains, protein secondary structure, and 3D structure of the putative HsHDR1 and HsHDR2 proteins were analyzed. The full-length cDNA of the HsHDR1 gene contained 1431 bp encoding a putative protein with 476 amino acids, whereas the HsHDR2 gene contained 1428 bp encoding a putative protein of 475 amino acids. These two proteins contained the conserved domain of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (PF02401), but without the transmembrane region and signal peptide. The most abundant expression of HsHDR1 and HsHDR2 was detected in H. serrate roots, followed by the stems and leaves. Our results provide a foundation for exploring the function of HsHDR1 and HsHDR2 in terpenoid and sterol biosynthesis in Huperziaceae plants. |
topic |
1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase Huperzia serrate Terpenoid Gene clone Bioinformatics analysis |
url |
http://www.sciencedirect.com/science/article/pii/S2211383515001343 |
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