Investigation of Antibacterial Effects of Peganum harmala Seed and STRAW Smokes In Vitro

• Main Authors: Neda Parvin, Kourosh Ashrafi, Najmeh Shahinfard, Zahra Keivani Hafshejani
• Format: Article
• Language: English
• Published: Institue of Medicinal Plants, ACECR 2011-11-01
• Series: Journal of Medicinal Plants
• Subjects: antimicrobial; bacteria; dung
• Online Access: http://jmp.ir/article-1-2906-en.html

Background: Encounter with drug resistance and replacment natural treatment is essential. From olden of Iran and specially Chaharmahal and bakhtiari Province medicinal smokes such as donkey dung & Peganum harmala seed used for treatment of many diseases like Infectious diseases. Regarded to lake of scientific research about antimicrobial effects of this smoke this study was done with aim of comparison of antimicrobial property of donkey dung and Peganum harmala seed smokes on different bactrial strains. Methods: In this interventional study, Esherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Enterococcus Faecalis and Candida albicans standard strain in suitable medium were cultured. Antibiobiogram blank disks were fumigated separately with Peganum harmala seed smoke, male and female donkey dung and straw smoke then placed on microbial plate with sterile method. After 48 hours incubation in 37 °C, evaluated by measuring the zone of growth inhibition surrounding the disks. Data were analyzed with SPSS 15 and mean statistic exam. Results: Staphylococcus aureus was sensitive to Peganum harmala seed, male donkey dung and female donkey dung smokes, Esherichia coli to male donkey dung smoke, Pseudomonas aeroginosa to male and female donkey dung smokes and Candida albicans to female donkey dung smoke. In control group Staphylococcus aureus and Enterococcus Faecalis were resistant against cloxacilllin and Pseudomonas aeroginosa was sensitive only to erythromycin and ciprofloxacin. Results of this study showed that by increasing the time of fumigation in sensitive cases, the antimicrobial effect increases. Conclusion: According to antimicrobial effects of male and female donkey dung smokes on resistance pathogens such as Pseudomonas aeroginosa and Staphylococcus aureus, necessity of perform expanded research about composition and property of this smokes revealed.