Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line
Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for g...
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Associação Brasileira de Divulgação Científica
2010-08-01
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doaj-e2b4bbc7612b4db8b5a4e93643064f912020-11-24T23:27:29ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2010-08-01438717721Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell lineH.F. RibeiroD.F.A. AlcântaraL.A. MatosJ.M.C. SousaM.F. LealM.A.C. SmithR.R. BurbanoM.O. BahiaGastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG-100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800004CarcinogenesisChromosomal abnormalitiesGastric cancerOncogenes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
H.F. Ribeiro D.F.A. Alcântara L.A. Matos J.M.C. Sousa M.F. Leal M.A.C. Smith R.R. Burbano M.O. Bahia |
spellingShingle |
H.F. Ribeiro D.F.A. Alcântara L.A. Matos J.M.C. Sousa M.F. Leal M.A.C. Smith R.R. Burbano M.O. Bahia Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line Brazilian Journal of Medical and Biological Research Carcinogenesis Chromosomal abnormalities Gastric cancer Oncogenes |
author_facet |
H.F. Ribeiro D.F.A. Alcântara L.A. Matos J.M.C. Sousa M.F. Leal M.A.C. Smith R.R. Burbano M.O. Bahia |
author_sort |
H.F. Ribeiro |
title |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_short |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_full |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_fullStr |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_full_unstemmed |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_sort |
cytogenetic characterization and evaluation of c-myc gene amplification in pg100, a new brazilian gastric cancer cell line |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2010-08-01 |
description |
Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG-100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor. |
topic |
Carcinogenesis Chromosomal abnormalities Gastric cancer Oncogenes |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800004 |
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